Báo cáo khoa học: Molecular organization and force-generating mechanism of dynein pot

... 2011 FEBS 2979 MINIREVIEW Molecular organization and force-generating mechanism of dynein Hitoshi Sakakibara 1 and Kazuhiro Oiwa 1,2 1 National Institute of Information and Communications Technology, ... clarify the mechanism of the coordination, modulating the tail domain could be one of the mechanisms of regulation of dynein motility [94]. In addition, dynei...

Ngày tải lên: 14/03/2014, 22:20

... concentration of Rep245 pre- pared in 10 mm sodium phosphate (pH 7.4) was 5 lm. CD spectra were recorded at 60, 70 and 80 °C between 190 and 260 nm with a step increase of 0.2 nm, and a bandwidth of 1 ... presence of 0.2 mm dNTPs and 0.5 U of Taq DNA polymerase (Promega). To analyze the effect of temperature on primase activity, Rep245 and Rep516 proteins were incubated...

Ngày tải lên: 18/02/2014, 18:20

... isoform without the insertion sequence and a molecular mass of 60 kDa (S70040) exists at a much higher expression level in the tomato fruit [21]. The allergenicity of b-fructofuranosidase of ... specific 5¢ -and 3¢ primers selected on the basis of the published sequences for tomato b-fructofuranosidase (GenBank accession no. D11350 and S70040). Primers for the short isoform of...

Ngày tải lên: 21/02/2014, 00:20

... 1. Molecular forms of Scpep1. (A) Analysis of molecular forms of Scpep1: 100 lg of cell lysates (C) and 50 lL of medium (M) of HT1080 and HT1080-Scpep1 were separated by SDS-PAGE, blotted and ... proteins in LSDs and a notable number of LSD-like diseases of unknown etiology, there is a com- mon interest in the identification of the proteome of the lysosomal comp...

Ngày tải lên: 07/03/2014, 03:20

... initial denaturation at 94 °C for 30 s; five cycles of 94 °Cfor5sand72°C for 4 min; five cycles of 94 °Cfor 5sand70°C for 4 min; and 20 cycles of 94 °Cfor5sand 68 °C for 4 min. The RACE–PCR product was ... (A) and presence of b 1 (B) and b 1B (C) subunits. Sodium currents were evoked by 15 ms long depolarizing pulses (as indicated) from a holding potential of )80 mV. (D) The effe...

Ngày tải lên: 16/03/2014, 23:20

... Zhi-Hui Zhu 2 and Hui Liu 1 1 Department of Biotechnology, College of Life Sciences, Wuhan University, China; 2 Department of Cardiology, Tongji Hospital, Huazhong University of Science and Technology, ... vein injection of 200 lL, subcutaneous injection of 2 mL, or intracerebroventricular Ó FEBS 2002 Molecular cloning and function of gene BmKIM (Eur. J. Biochem. 269) 44...

Ngày tải lên: 31/03/2014, 09:20

... measure- ment of k* on and k* off . At [LCTI] ⁄ [trypsin] ¼ 1.1 and 2.25 the conversion of LCTI into LCTI* (Fig. 3, curves 2 and 3), allowed the simultaneous computation of k off , k* on and k* off . ... to 135 min )1 and to 3.85 min )1 and K M values of 1250 lm and 850 lm for BApNA and GPpNA, respectively, by means of standard Lineweaver–Burk analysis of initial r...

Ngày tải lên: 23/03/2014, 10:21

... Lluis Màrquez, and Joakim Nivre. 2008. The CoNLL-2008 Shared Task on Joint Parsing of Syntactic and Semantic Dependencies. In Pro- ceedings of CoNLL 2008. Charles Sutton and Andrew McCallum. ... pre- cision of 87.6 and recall of 65.8). There have been other efforts in NLP on joint learning with various degrees of success. In par- ticular, the recent shared tasks of C...

Ngày tải lên: 23/03/2014, 16:20

... S1. Comparison of intron–exon structures of GmPDIL-3a, GmPDIL-3b and orthologs of other plants. Fig. S2. Multiple amino acid sequence alignment of GmPDIL-3a, GmPDIL-3b and orthologs of other plants. Fig. ... Identification of the initiation codons in GmPDIL-3a and Gm-PDIL-3b mRNAs. (A) Schematic representation of the structure of GmPDIL-3a and GmPDIL-3b mRNAs. The putat...

Ngày tải lên: 18/02/2014, 11:20

... region between b and b¢ in GmPDIL-1, and between b and b¢ and b¢ and a¢ in GmPDIL-2, the structure of these regions may be protease resistant. The activity of recombinant GmPDIL-1 and GmP- DIL-2, ... In the case of GmPDIL-2, 10 of the 11 introns had TTNAN, and the eighth intron possessed CTNAN. Promoter regions of around 1 kb and 1.5 kb upstream of each start codon...

Ngày tải lên: 07/03/2014, 05:20