Báo cáo khoa học: Solution and membrane-bound chaperone activity of the diphtheria toxin translocation domain towards the catalytic domain" doc

... equilib-rium of the C domain in favor of the MG state. The T domain favors the interaction of the C domain with the membrane, because it brings the C domain in its MG state into the vicinity of the bilayer, ... intoxication by diphtheria toxin, endosome acidiﬁcation trig-gers the translocation of the catalytic (C) domain into the cytoplasm. Thisevent is mediated by the translocation (T) domain of the toxin. ... receptor-binding domain) , each of these steps. The behavior of the T domain is marginallymodiﬁed by the presence or absence of the C domain, whereas that of the C domain is greatly affected by the presence of...

Tài liệu Báo cáo khoa học: Molecular and cellular speciﬁcity of post-translational aminoacyl isomerization in the crustacean hyperglycaemic hormone family docx

... terminal in the sinus gland, as a result of late and progressiveisomerization of the Phe3 of the CHH during the migration of the secretion vesicles along the axonaltract [39,41]. The immunohistochemical ... microscopystudy of the neuronal endings in the sinus gland todetermine the subcellular localization of the differentepimers. Taken together, these results allow a discus-sion of the existence of enzyme(s) ... (not shown). Therefore, the results obtainedindicated that the two VIH isomers differed by the conﬁguration of the tryptophan at the fourth position of the sequence and, consequently, they were...

Tài liệu Báo cáo khoa học: Structural and functional speciﬁcities of PDGF-C and PDGF-D, the novel members of the platelet-derived growth factors family docx

... removal of the CUBdomains and thereby activation of the growth factordomains. PDGF-C and -D contain both the CUB and growth factor domains when they are secreted and pro-teolytic cleavage is therefore ... model of the PDGF-C growth factor domain indicates the disulﬁdebridges in PDGF-C to consist of Cys250 and 294,Cys280 and 335, and Cys287 and 337, and the inter-monomeric bonds to consist of Cys274 ... a wide scope of biological effects;however further understanding of how these factorsinterplay with other members of the cystine knot family and in particular the PDGF-A, -B, and VEGF growthfactors,...

Báo cáo khoa học: Energetic and metabolic transient response of Saccharomyces cerevisiae to benzoic acid docx

... that the response time of the dissolved O2probe is normally smaller than the responsetime of the off-gas measurement, for which the contribution of the dilution in the fermentor headspace, the ... length of tubing connecting the fermentor with the off-gas analyzer and the response time of the off-gas analyzer itself shouldbe accounted for. Consequently, the OUR reconstructedfrom the combined ... permeability to these acids [13]. Thisreduces the passive diffusion of non-dissociated acid,limits the inﬂux of these weak acids and reduces theireffects on cell metabolism. An overview of these adap-tation...

Báo cáo khoa học: NMR and molecular dynamics studies of an autoimmune myelin basic protein peptide and its antagonist Structural implications for the MHC II (I-Au)–peptide complex from docking calculations ppt

... elucidate the role of the positively charged residue Arg78 and the effect of the reduction of cationicity of MBP in the triggering of multiplesclerosis. Flexible docking calculatio ns of the MBP(74–85)epitope ... eycharacteristic of the structure of the native epitope inMe2SO is the presence of a large number of electrostaticinteractions, especially in the central region of the peptide. The side chain of Arg78 ... role in the Fig. 4. Backbone cross RMSD matrix for the comparison of the various conformers of the ﬁve MD simulations of Table 1. The x and y axescorrespond to the simulation time of the various...

Báo cáo khoa học: Covalent and three-dimensional structure of the cyclodextrinase from Flavobacterium sp. no. 92 pdf

... between the long B -domain extension of CDase(His251, Fig. 7) and the N-terminal domain of the othersubunit of the TVA-II dimer (Tyr45¢). It has been suggestedthat the N-terminal domain of the ... domains A and C of the othersubunit. In contrast, the TVA-II group dimer attaches the B domain to an N-terminal domain of the other subunit,which restricts the size of the B domain (Fig. 7).Active ... superposition of CDase (blue with light green domain B) with the TVA-II dimer (grey with dark green domain B and pink N-terminal domain) . The N-terminal domain of the other subunit of the TVA-II...

Báo cáo khoa học: Stability and ﬁbril formation properties of human and ﬁsh transthyretin, and of the Escherichia coli transthyretin-related protein potx

... polar residues, Thr75 and His88 (hTTR number-ing), buried within the hydrophobic core of the mono-mers. The side chains of these residues form, togetherwith the Ne1 atom of Trp79, hydrogen bonds ... hydrophobicity of molecules. Gel ﬁltration chromatography of ecTRP in the presence and absence of 0.5% SDS in the runningbuffer produced monomers only when the SDS wasincluded (data not shown). The thermal ... cold-adapted proteins and distinguishesthem from their mesophilic and thermophilic counter-parts is their lower thermal stability [66]. This couldtherefore be one explanation for the reduced stabilitythat...

Báo cáo khoa học: Substrate and inhibitor speciﬁcity of Mycobacterium avium dihydrofolate reductase pptx

... 4). The Km(NADPH) of the L32Dmutant was 0.84 lm, and therefore almost two-foldlower than that of the wild type. The wild type and the L32F and L32A mutants had Vmax(NADPH) val-ues of 80, ... affects the enzyme’s activity in M. avi-um, E. coli and L. casei. The D31L mutant of M. avium DHFR had < 1% of the wild-type rDHFR’s activity. The very low speci-ﬁc activity of the D31L ... in the secondary struc-ture of the protein. Even though the estimated struc-tures of the mutants D31Q and L32D do not differsigniﬁcantly from the structure of the wild-type pro-tein, their...

Báo cáo khoa học: High and complementary expression patterns of alcohol and aldehyde dehydrogenases in the gastrointestinal tract Implications for Parkinson’s disease docx

... gene activity is observed in the epithelial cells of the tongue and esophagus, in the gastric glands and at the base of the villi in jejunum. The apparent signal in the outer keratinized layer of ... epithelial cells at the base of the villi.In colon, Aldh1 gene activity was detected in the upperparts of the crypts of Lieberku¨hn, whereas the lowerparts of the crypts, the submucosa and ... insignal intensity observed towards the superﬁcial layers.In the stomach, Aldh1 mRNA was present in the epi-thelial cells of the gastric pits and in the neck of the gastric glands. Aldh1 expression...

Báo cáo khoa học: "Sentence and Expression Level Annotation of Opinions in User-Generated Discourse" potx

... fact=yes. If the sentence is a polar fact, then the aim is to mark the target and label the polarity of the evaluation. If the sentence is opinionated,then, the aim is to mark the opinion expressionspan, ... the expres-sion levelWe observe that the strength of the opinionex-pression and the polar target polarity cannot belabeled as reliably as the polarity of the opinion-expression. 61% of the ... interpreting the opinions and evaluations inuser-generated discourse. Due to the high popular-ity of Web 2.0 communities1, the amount of user-generated discourse and the interest in the analysisof...

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