Báo cáo khoa học: Solution and membrane-bound chaperone activity of the diphtheria toxin translocation domain towards the catalytic domain" doc

... equilib- rium of the C domain in favor of the MG state. The T domain favors the interaction of the C domain with the membrane, because it brings the C domain in its MG state into the vicinity of the bilayer, ... intoxication by diphtheria toxin, endosome acidification trig- gers the translocation of the catalytic (C) domain into the cytoplasm....

Ngày tải lên: 14/03/2014, 22:20

... terminal in the sinus gland, as a result of late and progressive isomerization of the Phe3 of the CHH during the migration of the secretion vesicles along the axonal tract [39,41]. The immunohistochemical ... microscopy study of the neuronal endings in the sinus gland to determine the subcellular localization of the different epimers. Taken together, these...

Ngày tải lên: 18/02/2014, 11:20

... removal of the CUB domains and thereby activation of the growth factor domains. PDGF-C and -D contain both the CUB and growth factor domains when they are secreted and pro- teolytic cleavage is therefore ... model of the PDGF-C growth factor domain indicates the disulfide bridges in PDGF-C to consist of Cys250 and 294, Cys280 and 335, and Cys287 and 337, an...

Ngày tải lên: 19/02/2014, 07:20

... that the response time of the dissolved O 2 probe is normally smaller than the response time of the off-gas measurement, for which the contribution of the dilution in the fermentor headspace, the ... length of tubing connecting the fermentor with the off-gas analyzer and the response time of the off-gas analyzer itself should be accounted for. Consequently,...

Ngày tải lên: 07/03/2014, 04:20

... elucidate the role of the positively charged residue Arg78 and the effect of the reduction of cationicity of MBP in the triggering of multiple sclerosis. Flexible docking calculatio ns of the MBP(74–85) epitope ... ey characteristic of the structure of the native epitope in Me 2 SO is the presence of a large number of electrostatic interactions, especia...

Ngày tải lên: 07/03/2014, 16:20

... between the long B -domain extension of CDase (His251, Fig. 7) and the N-terminal domain of the other subunit of the TVA-II dimer (Tyr45¢). It has been suggested that the N-terminal domain of the ... domains A and C of the other subunit. In contrast, the TVA-II group dimer attaches the B domain to an N-terminal domain of the other subunit, which re...

Ngày tải lên: 08/03/2014, 02:20

... polar residues, Thr75 and His88 (hTTR number- ing), buried within the hydrophobic core of the mono- mers. The side chains of these residues form, together with the Ne1 atom of Trp79, hydrogen bonds ... hydrophobicity of molecules. Gel filtration chromatography of ecTRP in the presence and absence of 0.5% SDS in the running buffer produced monomers only when the SD...

Ngày tải lên: 16/03/2014, 01:20

... 4). The K m (NADPH) of the L32D mutant was 0.84 lm, and therefore almost two-fold lower than that of the wild type. The wild type and the L32F and L32A mutants had V max (NADPH) val- ues of 80, ... affects the enzyme’s activity in M. avi- um, E. coli and L. casei. The D31L mutant of M. avium DHFR had < 1% of the wild-type rDHFR’s activity. The very lo...

Ngày tải lên: 16/03/2014, 10:20

... gene activity is observed in the epithelial cells of the tongue and esophagus, in the gastric glands and at the base of the villi in jejunum. The apparent signal in the outer keratinized layer of ... epithelial cells at the base of the villi. In colon, Aldh1 gene activity was detected in the upper parts of the crypts of Lieberku ¨ hn, whereas the lo...

Ngày tải lên: 16/03/2014, 11:20

... fact=yes. If the sentence is a polar fact, then the aim is to mark the target and label the polarity of the evaluation. If the sentence is opinionated, then, the aim is to mark the opinion expression span, ... the expres- sion level We observe that the strength of the opinionex- pression and the polar target polarity cannot be labeled as reliably as the polar...

Ngày tải lên: 16/03/2014, 23:20