... is the initial velocity of the reaction, Vmaxis the maximum velocity, [S] is the concentration of citrate, Ksis the dissociation constant of the enzyme-citrate (ES) com-plex, and a, b, and ... Ks1<<Ks2<<Ks3, binding of the first and second molecules of citrate drastically decrease the affinity of the enzyme for the second and third molecules,respectively. The dissociation constant of the first citratemolecule ... mechanism of mammalian ACL [23], the final step of the reaction can be assumed to be the nucleophilic attack of CoA to the phosphorylated carbonyl carbon of citrylphosphate, and the cleavage of the...