Gel Electrophoresis – Teacher Instructions
Gel Electrophoresis – Teacher Instructions
. Sciences Initiative Gel Electrophoresis – Teacher Instructions Suggested Grade Level: Grades 7-14 Class Time Required: 1 period (50 minutes) EQUIPMENT AND MATERIALS NEEDED (per group) • Electrophoresis. chamber, gel form and comb • Electrophoresis power supply • Agar gel • Salt solution • Scientific stains to use as samples • Sample-loading device • Masking tape, if needed to seal gel form...
Ngày tải lên: 13/03/2014, 19:38
Gel electrophoresis
. Agarose Gel Electrophoresis Experiment Procedures PREPARING THE GEL FOR ELECTROPHORESIS 11. After the gel is completely solidified, carefully and slowly remove the rubber dams or tape from the gel. Practice of Agarose Gel Electrophoresis Experiment Procedures Conducting Agarose Gel Electrophoresis ELECTROPHORESIS SAMPLES Samples in EDVOTEK Series 100 and Sci-On® Series electrophoresis experi...
Ngày tải lên: 23/02/2014, 11:10
Báo cáo khóa học: Characterization of presenilin complexes from mouse and human brain using Blue Native gel electrophoresis reveals high expression in embryonic brain and minimal change in complex mobility with pathogenic presenilin mutations pptx
. PS1 NT ( 1–2 0) [20]; Ab 00/ 1 and Ab 00/2 PS1 loop (30 1–3 17) [21]; Ab 00/12, PS2 loop (30 7–3 36); Ab 00/19 NCT CT (69 1–7 09]) [22]; Ab 00/22 NCT ectodomain (33 1–3 46); Ab 02/45 PEN-2 NT ( 1–1 5) and. mutant presenilin 1. Nature 383, 71 0–7 13. 34. Scha ¨ gger, H. (2001) Blue-native gels to isolate protein complexes from mitochondria. Methods Cell Biol. 65, 23 1–2 44. 35. Culvenor, J.G., Mc...
Ngày tải lên: 16/03/2014, 16:20
Báo cáo khoa học: Analysis of the CK2-dependent phosphorylation of serine 13 in Cdc37 using a phospho-specific antibody and phospho-affinity gel electrophoresis doc
. kinase–Hsp90–Cdc37 com- plexes by phospho-affinity gel electrophoresis Finally, we analyzed complexes between signaling pro- tein kinases and Hsp90–Cdc37 molecular chaperones by phospho-affinity gel. by phospho-affinity gel electrophoresis. (A) Recombinant Cdc37 alone (lanes 1–3 ), with CK2a (lanes 4–6 ) or with CK2 holoenzyme (lanes 7–9 ), in the pres- ence (+) or absence (–) of Mg 2+ and ⁄ or....
Ngày tải lên: 30/03/2014, 03:20
Thu nhận enzyme pectinase từ sp.niger - tinh sạch bằng phương pháp lọc gel và lọc màng
. Phân tích chế ph ẩm enzyme pectinase bằng phương pháp lọc gel Bio Gel P 30 Dung dịch enzyme xử lý theo mục 2.5, kết quả sau khi lọc gel với tốc độ chảy qua cột 30ml/giờ thu được 2 peak chính. được xác định bằng phương pháp Lowry[1] 2.5. Phân tích chế phẩm enzyme bằng phương pháp lọc gel Bio Gel P 30 TẠP CHÍ PHÁT TRIỂN KH&CN, TẬP 11, SỐ 08 - 2008 Trang 47 Hòa tan chế phẩm enzyme. ly...
Ngày tải lên: 15/09/2012, 18:22
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