chromatographic methods gel permeation

Ngày tải lên: 04/06/2014, 15:22

... section 2 they will be in breach of the law and liable for a fine of $25 000. Safe carrying methods Carrying heavy objects can put a strain on your back, arms, shoulders and knees because ... that are too heavy for you to lift or carry. In these cases, you may need to use some of these methods: • Work with someone to carry the load. • Use a trolley. • Use a forklift or trolley jack...

Ngày tải lên: 21/08/2012, 17:56

... Phân tích chế ph ẩm enzyme pectinase bằng phương pháp lọc gel Bio Gel P 30 Dung dịch enzyme xử lý theo mục 2.5, kết quả sau khi lọc gel với tốc độ chảy qua cột 30ml/giờ thu được 2 peak chính ... hiệu suất cao hơn nên chọn cột lọc gel và kích thước màng lọc phù hợp với từng loại enzyme. PURIFICATION OF PECTINASE FROM ASP.NIGER BY THE TECHNIQUES OF GEL FILTRATION AND CROSS-FLOW MEMBRANE ... inducer obtained the highest activity after 48 hours. Separating enzyme on a gel filtration column packed with Bio gel P 30, which showed that one peak contained activity of pectinase, and with...

Ngày tải lên: 15/09/2012, 18:22

... makes that it is largely used), and is given by: Master of Computer Science 1 - MOB Mobile Internet and Surrounding 1/12 Baey, Fladenmuller – Subject 5 MOB Subject 5 –Access methods Static and...

Ngày tải lên: 17/09/2012, 09:13

... centrifuged to separate the gel from the sol phase, allowing the identification of the gel- forming mucins. Repeated extraction of the gel phase solubi- lizes the “soluble” gel- forming species, leaving ... a better separation between mucins Isolation of Large Gel- Forming Mucins 3 3 From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins Edited by: A. Corfield ... Biochem. Mol. Biol. 27, 5792. Methods in Molecular Biology TM HUMANA PRESS Glycoprotein Methods and Protocols Edited by Anthony P. Corfield VOLUME 125 The Mucins Methods in Molecular Biology TM HUMANA...

Ngày tải lên: 23/09/2012, 19:25

... nonsulfated mucins remain unstained. Histological Methods for Detection of Mucin 29 29 From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins Edited by: A. ... tract. Histological Methods for Detection of Mucin 39 3. Alternatively, prepared adhesive slides such as Superfrost Plus ® /(Menzel-Gläser, Braunschweig, Germany) may be purchased. 4. There are many other methods ... A., and Katsuyama, T. (1997) Histochem- istry of the surface mucous gel layer of the human colon. Gut 40, 782–789. Histological Methods for Detection of Mucin 35 10. Antigen retrieval solutions:...

Ngày tải lên: 23/09/2012, 19:25

... Histologically-based methods for detection of mucin, in Glycoprotein Methods and Protocols: The Mucins (Corfield, T., ed.), Humana, Totowa, NJ. 2. Davies, R., Carlstedt, I. (2000) Isolation of large gel- forming ... separation. Polyacrylamide gels of a percentage that will not allow migration of high M r mucins even into the stacking gels are often used. Even at the lower limit of polyacrylamide gel formation (3%) ... Mucins can be detected by chemical methods within acrylamide Detection and Quantitation of Mucins 45 45 From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins Edited...

Ngày tải lên: 23/09/2012, 19:25

... unfixed mucosa (3). The first two of these methods also measure the GI Adherent Mucous Gel Barrier 59 unstirred aqueous layer beyond the adherent mucous gel surface, the extent of which depends ... mounted in a water-soluble gelatin gel. Values for mucous thickness over the rat gastric mucosa by this method give a mean of 147 µm, which approximates that of the adherent gel remaining after suction ... provided by wrapping them in a 1–cm 2 sand- GI Adherent Mucous Gel Barrier 57 57 From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins Edited by: A. Corfield...

Ngày tải lên: 23/09/2012, 19:25

... apparatus to measure radioactivity in flat materials (gels or filter paper) by autoradiography. It calculates the amount of radioactivity in a band on gel or on a dot on filter paper, taking into account ... PhosphorImager as au/A 260nm . Biosynthesis and Secretion of Mucin 65 65 From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins Edited by: A. Corfield © Humana Press ... Trizol RNA isolation solution (Gibco/BRL, Gaithersburg, MD). 10. Agarose gel electrophoresis apparatus, and 0.8% (w/v) agarose gels. 11. Radiolabeled, homologous cDNA or cRNA probe to quantify the...

Ngày tải lên: 23/09/2012, 19:25

... × 10 6 ) of the gel- forming mucins in particular, there are few separation techniques available to use with these 77 From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: ... perchlorate. 2.3.2. Agarose Gel Electrophoresis 1. Horizontal electrophoresis apparatus, e.g., Bio-Rad DNA subcell Model 96 (25 × 15 cm gels) and the Bio-Rad subcell (15 × 15 cm gels). 2. Agarose (ultrapure). 3. ... culture, focusing primarily on those mucins involved in gel formation. At present, the mucins MUC2, MUC5AC, MUC5B, and MUC6 are known to be gel- forming molecules (1–4). These mucins share common features...

Ngày tải lên: 23/09/2012, 19:26

... Subheading 2.3.1. 3. Methods 3.1. Extraction and Purification For a detailed description, see Chapter 1. 3.2. Rate-Zonal Centrifugation Because of their extreme size, most gel chromatographic media ... (see Note 5). Heterogeneity and Size Distribution of Gel- Forming Mucins 87 87 From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins Edited by: A. Corfield ... biologi- cal property of gel- forming mucins and a key comparative characteristic when studying the change of mucous properties through the course of disease. This chapter describes only methods for assessing...

Ngày tải lên: 23/09/2012, 19:26

... code as defined in Fig. 1. Amino Acid Analysis of Mucins 113 113 From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins Edited by: A. Corfield © Humana Press ... desalting column), or the mucin can be dot-spotted onto polyvinylidene difluoride (PVDF) membrane, or gel separated by electrophoresis and electroblotted onto PVDF. Note that mucins are often difficult ... Mobile phase B: 15% (v/v) methanol in water. 6. Mobile phase C: 90% (v/v) acetonitrile in water. 3. Methods 3.1. Sample Preparation and Hydrolysis 1. Add an aliquot of a solution of a sample of mucin...

Ngày tải lên: 23/09/2012, 19:26

... Sequencing Glycopeptides 121 121 From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins Edited by: A. Corfield © Humana Press ... Elsewhere we have published alternative HPLC methods for the separation of PTH-glycosylated amino acids on a modified Beckman LF3600 sequenator (12). These methods were developed for two reasons: ... with high molecular weight, heavily glycosylated domains such as those found in mucins. The two methods for determining the sites of glycosylation in proteins are mass spectrometry and Edman sequencing....

Ngày tải lên: 23/09/2012, 19:26

... R Trityl C Synthetic Peptides for Antimucin Antibodies 129 129 From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins Edited by: A. Corfield © Humana Press ... than peptide alone. Glyco- peptides can be produced by both chemical (12) and enzymatic (13,14) methods. These reagents have been of value in assessing the contribution of O-linked N-acetyl- galactosamine ... cannot be controlled or simply that short peptides do not adhere well to polystyrene plates. Several methods of peptide modification have been utilized to overcome these problems. One such procedure...

Ngày tải lên: 23/09/2012, 19:26

... Blot Analysis 1. Tris-glycine polyacrylamide gels (precast) (Novex, San Diego, CA). 2. Gel electrophoresis apparatus (Novex Xcell II Mini Cell). 3. Gel running buffer: 25 mM Tris, 192 mM glycine, ... 100 min, or until the bromophenol blue dye reaches the bot- tom of the gel. 5. Transfer the separated proteins from the gel to a nitrocellulose membrane at a constant voltage of 100 V for 1 h ... on polyacrylamide gels. The percentage of polyacrylamide used will vary with the size of the expected product and the desired separation. Most experiments in the present study were performed using 8% gels. 1....

Ngày tải lên: 23/09/2012, 19:26

... can also occur (Fig. 1) (1,2). Most of the substituents, largely O-acetyl groups are quite labile during acid or alkaline hydrolysis methods generally utilized for the release of monosaccharides. ... (Kdn), respectively. Substituents of the hydroxyl groups present on From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins Edited by: A. Corfield © Humana Press ... a suitable camera system. 1. Place the gels on a UV transilluminator with maximum emissions at wavelengths of ei- ther 254 or 365 nm. 2. Photograph the gels through a yellow filter. Monosaccharide...

Ngày tải lên: 23/09/2012, 19:26

Ngày tải lên: 23/09/2012, 19:26

Ngày tải lên: 23/09/2012, 19:26

Ngày tải lên: 23/09/2012, 19:26

Ngày tải lên: 23/09/2012, 19:26