Prepare of mRNA - Tài liệu

Báo cáo khoa học: A new molecular tool for transgenic diatoms Control of mRNA and protein biosynthesis by an inducible promoter–terminator cassette docx

... completely shut off, the expression of genes that are under control of the Pnr ⁄ Tnr cassette. Decoupling of transcription and translation of gfp mRNA To further evaluate the properties of the Pnr ... comparable rate of increase of GFP ﬂuorescence (2 h doubling time) after A B Fig. 4. Inﬂuence of nitrogen source on mRNA expression and the formation of GFP protein. (A) Fl...

Ngày tải lên : 07/03/2014, 21:20

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Influence of Polyferric Sulfate Coagulant on the amoA mRNA Expression of Ammonia Oxidizer in Activated Sludge

... of cDNA of amoA mRNA. The profiles of T-RFLP in Run 2 are shown in Fig. 4. Table 1 shows the species of ammonia oxidizer of the major T-RFs (219, 283, 354 and 491 bp). Although almost all of ... addition of polyferric sulfate does not affect the number of ammonia oxidizer, instead, the amount of amoA mRNA is affected in a short time. Because amoA mRNA is one of t...

Ngày tải lên : 05/09/2013, 10:15

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Tài liệu Control the Creation and Behavior of Classes Now that you have code that can prepare all of the pdf

... developer of the problem. Of course, a developer could ignore your exception and attempt to access the properties of the object, but you did give those other developers ample warning. Also, none of ... instance of the class could exist in your application. In short, in VB 6, you didn't have control over the creation of objects. Technique Visual Basic .NET does give you thi...

Ngày tải lên : 24/12/2013, 06:17

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Tài liệu Báo cáo khoa học: Interleukin-1-inducible MCPIP protein has structural and functional properties of RNase and participates in degradation of IL-1b mRNA doc

... regulates the amount of IL-1b mRNA. Involvement of PIN domain of MCPIP in the stability of IL-1 b mRNA In order to ﬁnd out whether the PIN domain in MCPIP is responsible for IL-1b mRNA regulation, ... exogenous) starts degradation of IL-1b mRNA and also of its own tran- script. For this reason, the level of mRNA for the wild- type form of MCPIP and the level of mR...

Ngày tải lên : 18/02/2014, 14:20

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Tài liệu Báo cáo khoa học: Regulation of connective tissue growth factor (CTGF/CCN2) gene transcription and mRNA stability in smooth muscle cells Involvement of RhoA GTPase and p38 MAP kinase and sensitivity to actin dynamics docx

... CTGF/ CCN2 mRNA levels were normalized to those of GAPDH and the graphical representation of the results of phosphorimage scans of the mRNA hybridization signals is shown as well. To compare mRNA expression ... relative amounts of normalized mRNA were plotted as a function of time and the slope of this curve was used to calculate the interval period within which half of...

Ngày tải lên : 19/02/2014, 16:20

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Tài liệu Báo cáo khoa học: Distribution of class I, III and IV alcohol dehydrogenase mRNAs in the adult rat, mouse and human brain ppt

... granular layer of human cerebellum. Table 3 compiles our findings in adult brain tissue of all three species. Discussion The distribution of ADHs in the brain is of particular interest because of their ... different regions of cortex cerebri. The cellular distribution of ADH3 protein coincides with our findings of mRNA: cortical neurons in deeper layers, hippocampal neurons and...

Ngày tải lên : 21/02/2014, 00:20

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Tài liệu Báo cáo Y học: Inhibition of nuclear pre-mRNA splicing by antibiotics in vitro pot

... the pre -mRNA, s uggesting that t he inhibition is the r esult of disturbance of t he correct foldin g o f the pre -mRNA into the splicing-compatible tertiary structure by the charged groups of these ... intron r emoval from pre -mRNA by a two-step transesteri®cation reaction occurs. The ®rst step of nuclear p re -mRNA splicing is g overned by a nucleophilic attack of the 2¢ OH...

Ngày tải lên : 21/02/2014, 15:20

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Báo cáo khoa học: Expression and physiological role of CCN4⁄Wnt-induced secreted protein 1 mRNA splicing variants in chondrocytes potx

... School of Medicine, Dentistry and Pharmaceutical Sciences, Japan 3 Division of Orthodontics and Dentofacial Orthopedics, Tohoku University Graduate School of Dentistry, Miyagi, Japan 4 Department of ... sequences of exon– intron boundaries of WISP1vx strictly conserve the general rule of mRNA splicing sites in higher eukaryo- tes (Fig. 2B). These ﬁndings indicate that WISP1vx...

Ngày tải lên : 07/03/2014, 10:20

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Báo cáo khoa học: A simple in vivo assay for measuring the efﬁciency of gene length-dependent processes in yeast mRNA biogenesis doc

... in the presence of sublethal amounts of MPA. (B) mRNA levels of the transcription units and strains ana- lyzed in (A) cultured in the absence of MPA. The relative values of the mRNA levels with ... steps of transcription inﬂuence equally the expression of the reporters, independently of the length of their 3¢-UTR. Results An in vivo assay to measure gene length- dependen...

Ngày tải lên : 07/03/2014, 12:20

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Báo cáo khoa học: The 3¢-UTR of the mRNA coding for the major protein kinase C substrate MARCKS contains a novel CU-rich element interacting with the mRNA stabilizing factors HuD and HuR ppt

... that the high expression of members of the ELAV/Hu family is responsible for the MARCKS mRNA stability in PCC7- Mz1 cells. Role of ELAV/Hu in controlling stability of MARCKS mRNA We have demonstrated ... nucleotides [(CUUU) 11 U 8 ]ofthe3¢-UTR of the MARCKS mRNA confers RNA instability when merged with a reporter gene; (b) this CU-rich sequence of 52 nucleotides of the MAR...

Ngày tải lên : 08/03/2014, 08:20

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