tRna
Tài liệu RNA vận chuyển (Transfer RNA - tRNA) ppt
... RNA - tRNA) M i tRNA g n v i m t phân t aminoỗ ắ ớ ộ ử acid, mang đ n ribosome đ tham giaế ể t ng h p protein. M i tRNA đ c hi uổ ợ ỗ ặ ệ cho m t lo i amino acid. Có h n 20 lo iộ ạ ơ ạ tRNA ... i, t o ra các tRNA m i.ế ổ ạ ớ Các tRNA cùng tham gia v nậ chuy nể m tộ acid amin là các izoaceptor. S l ng izoaceptor thay đ iố ượ ổ tùy acid amin. C u trúc b c I c a tRNA: tRNA v...
Ngày tải lên: 14/12/2013, 21:16
Tài liệu Báo cáo khoa học: Modeling of tRNA-assisted mechanism of Arg activation based on a structure of Arg-tRNA synthetase, tRNA, and an ATP analog (ANP) ppt
... the Arg -tRNA synthetase-catalyzed deacylation of Arg- tRNA (Arg -tRNA + AMP fi Arg-AMP + tRNA at high pH), in which the deacylation of aminoacyl -tRNA bound on Arg -tRNA synthetase and Glu -tRNA synthetase ... ATP–pyrophosphate exchange reaction catalyzed by Arg -tRNA, Gln- tRNA and Glu -tRNA synthetases requires the assistance of the cognate tRNA. tRNA also assists Arg -tRNA...
Ngày tải lên: 18/02/2014, 11:20
Tài liệu Báo cáo khoa học: Amino acid discrimination by arginyl-tRNA synthetases as revealed by an examination of natural specificity variants doc
... bean transcript Soybean transcript Wheat germ total tRNA Bovine liver total tRNA E.coli native tRNA- Arg E.coli transcript Source of tRNA level noitalynigrA htiw deniatta %( iloc.E )emyzne Fig. 6. Interspecies arginylation. tRNA from ... class Ia amino- acyl -tRNA synthetase is crucial for tRNA binding. Biochemistry 42, 15092–15101. 35 Delagoutte B, Moras D & Cavarelli J (20...
Ngày tải lên: 18/02/2014, 13:20
Tài liệu Báo cáo khóa học: The unusual methanogenic seryl-tRNA synthetase recognizes tRNASer species from all three kingdoms of life pptx
... (type 2 tRNAs; tRNA Ser ,tRNA Leu and tRNA Tyr ), eukaryotic cytoplasm and archaea have only two (tRNA Ser and tRNA Leu ) [7–9]. Experimental evidence revealed different mechanisms of type 2 tRNA ... aminoacylation of 0.33 l M tRNAs: 1, M. maripaludis tRNA Ser ;2,M. maripaludis tRNA Sec ;3,M. jannaschii tRNA Ser ;4, M. jannaschii tRNA Sec ;5,E. coli tRNA Ser ;6,S. cerevis...
Ngày tải lên: 19/02/2014, 12:20
Tài liệu Báo cáo khoa học: Transcription of individual tRNA1Gly genes from within a multigene family is regulated by transcription factor TFIIIB pdf
... groups; 2 for tRNA Gly 1 -1, 3 for tRNA Gly 1 -4 and 4 for tRNA Gly 1 -6,7) The transcripts arising from each of the tRNA Gly 1 genes were differentially quantified. Filled bars, tRNA Gly 1 -1; ... Transcription levels of tRNA Gly 1 -4 were 40–60% that of tRNA Gly 1 -1 and < 10% that of tRNA Gly 1 -6,7 (Fig. 4C, first three bars grouped together). Transcripts from tRNA Gl...
Ngày tải lên: 20/02/2014, 02:21
Tài liệu Báo cáo Y học: Novel complexes of mammalian translation elongation factor eEF1AÆGDP with uncharged tRNA and aminoacyl-tRNA synthetase potx
... during vectorial transfer of tRNA/ aminoacyl -tRNA in the eukary- otic elongation cycle [13]: [ribosomal E site tRNA] (1) fi [eEF1AÆGDP tRNA] (2) fi [eEF1AÆGDP tRNA] ÆARS (3) fi [eEF1AÆGTPÆaminoacyl -tRNA] (4) fi [ribosomal AsiteÆaminoacyl -tRNA] ... EF1AÆGTP preparation was used immediately. tRNA Phe purification Enriched tRNA Phe preparation was obtained from crude rabbit liver tRNA by B...
Ngày tải lên: 21/02/2014, 15:20
Báo cáo khoa học: Flexibility and communication within the structure of the Mycobacterium smegmatis methionyl-tRNA synthetase Henrik Ingvarsson and Torsten Unge potx
... glutaminyl -tRNA synthetase tRNA Gln complex [E. coli GlnRS :tRNA Gln ; Protein Data Bank identifica- tion (PDB ID): 1QTQ] revealed that the MSK Lys residue (amino acid 270 in E. coli GlnRS :tRNA Gln ) hydrogen ... acid region supporting specific methionyl -tRNA synthetase: tRNA recognition. J Mol Biol 208, 429–443. 21 Deniziak MA & Barciszewski J (2001) Methionyl -tRNA synthetase....
Ngày tải lên: 06/03/2014, 22:21
Báo cáo khoa học: Kinetic and mechanistic characterization of Mycobacterium tuberculosis glutamyl–tRNA synthetase and determination of its oligomeric structure in solution pptx
... glutamyl tRNA synthetase; DLS, dynamic light scattering; E, total enzyme concentration; Ec-GluRS, Escherichia coli glutamyl tRNA synthetase; GlnRS, glutaminyl tRNA synthetase; GluRS, glutamyl– tRNA ... glutamyl tRNA synthetase (Mt- GluRS). Glutamyl tRNA synthetases (GluRS) belong to the broad class of aminoacyl tRNA synthetases (aaRS), which catalyse the essential charging reaction of...
Ngày tải lên: 07/03/2014, 03:20
Báo cáo khoa học: Eukaryotic class 1 translation termination factor eRF1 ) the NMR structure and dynamics of the middle domain involved in triggering ribosome-dependent peptidyl-tRNA hydrolysis pptx
... peptidyl -tRNA ester bond and that the conserved adjacent Gly and neighboring basic residues facilitate contact with the phosphate backbone of either rRNA and ⁄ or the accep- tor stem of the P site tRNA ... resembling the letter ‘Y’, partly mimics the ‘L’-shape of the tRNA molecule, and the M domain of eRF1 is equivalent to the acceptor stem of a tRNA [29]. It has also been suggested t...
Ngày tải lên: 07/03/2014, 05:20
Báo cáo khoa học: Characterization of the tRNA and ribosome-dependent pppGpp-synthesis by recombinant stringent factor from Escherichia coli pot
... twofold excess of tRNA Met f sti- mulated SF to the same extent upon addition of tRNA Phe to the activity assay (results not shown). Binding of tRNA Met f to ribosomes Binding of tRNA Met f to T4-mRNA ... A-site was saturated with unacylated tRNA. Is the tRNA- stimulatory effect affected by the order of addition of tRNA and SF to the reactions? The two types of tRNA titration e...
Ngày tải lên: 07/03/2014, 16:20