trnagln binding to glutaminyl trna synthetase

... that the observed heterogeneity in binding of [125I]TC-PCSK9 to HepG2 cells is attributable to binding to different populations of LDLR that exist prior to ligand binding (see below) Previous Biacore ... of the receptor via a two-step reaction in which the first binding step, representing binding to the EGF-A domain, is followed by binding of PCSK9 to a second site within the receptor In this ... represent binding of PCSK9 to dimeric receptors that release bound ligand slowly because they convert rapidly The slow phase of association could represent binding to monomeric receptors that...

Ngày tải lên: 14/02/2014, 14:20

... response to selective pressures arising from individual environmental conditions According to the crystal structures reported here, PhyK seems to have a preformed substrate -binding site and to be ... was added to PhyK in order to facilitate His-tag affinity purification The three distinct groups of HAPs are adapted to different habitats To support plant growth, bacteria not need to release ... whereas alkaline phosphatases are only able to hydrolyze three phosphate groups resulting in myo-inositol triphosphates as product In addition to their ability to make inorganic phosphorus available...

Ngày tải lên: 16/02/2014, 09:20

... thermophilus Val -tRNA synthetase bound to tRNAVal (Protein Data Bank ID: 1GAX) [17] and T thermophilus Leu -tRNA synthetase bound to tRNALeu (Protein Data Bank ID: 2BYT) [26], tRNA is left in the ... reveals large movements of these domains [12] Binding site of adenosine of ATP In Met -tRNA, Ile -tRNA, Val -tRNA and Leu -tRNA synthetases [15–18] belonging to class Ia, crystal structures of complexes ... Yokoyama S (2003) ATP binding by glutamyl -tRNA synthetase is switched to the productive mode by tRNA binding EMBO J 22, 676–688 Deutscher MP (1967) Rat liver glutamyl ribonucleic acid synthetase J Biol...

Ngày tải lên: 18/02/2014, 11:20

... transcript germ total liver total tRNA- Arg tRNA tRNA Source of tRNA Fig Interspecies arginylation tRNA from the sources indicated were arginylated in the presence of arginyl -tRNA synthetase from ... the glutamyl- and glutaminyl- tRNA synthetases of E coli For glutamyl -tRNA synthetase, the presence of tRNA eliminates non-specific binding of d-glutamic acid and l-aspartic acid to the enzyme [9,10] ... arginyl -tRNA synthetase, rearrangement of the enzyme active site appears to rely on additional discriminatory elements within the tRNA structure to ensure accurate formation of aminoacyltRNA This...

Ngày tải lên: 18/02/2014, 13:20

... terbium binding to this crystallin by ITC and determined the binding constant for the calcium mimic probe Terbium is believed to bind strongly to calcium -binding sites of proteins compared to calcium ... calcium -binding members of the bc-crystallin superfamily [14,15,20] Based on our results, together with the published data on calcium -binding to a few other members, we suggest that calcium -binding ... for further calcium -binding studies, otherwise the proteins were stored frozen at )80 °C Calcium -binding to bB2- and bA3-crystallins Because there is no known motif for calcium -binding in bB2- and...

Ngày tải lên: 18/02/2014, 16:20

... regulation occurs by binding of the inhibitor to an allosteric site in competition with the activator dCTP [7] For dCTP deaminase, the mechanism of dTTP regulation is not understood Only dCTP deaminases ... an underestimation of Vss dTTP binding to dCTP deaminase was also investigated by equilibrium binding This revealed a hyperbolic binding curve (Fig 4D) with a stochiometry of : of dTTP bound ... regulatory point of view dCMP deaminase is activated by dCTP and inhibited by dTTP and both nucleotides act on the enzyme by binding to an allosteric site to alter the cooperativity of dCMP binding...

Ngày tải lên: 18/02/2014, 16:20

... phosphorylation [33] Other cytoplasmic domains related to multichain immune recognition receptors were found to be intrinsically disordered even when bound to lipids [36] A role of the cytoplasmic tail of ... from 355 to 345 nm (Fig 5) Altogether, fluorescence data confirm binding of J1_tmic to SDS micelles and DMPG phospholipid vesicles, with at least partial embedding of one or both the tryptophan residues ... function of their cytoplasmic tails and, to our knowledge, only few examples have been reported [32,33] The cytoplasmic tail of the T-cell receptor f-chain [34,35] binds to lipid membranes through...

Ngày tải lên: 18/02/2014, 16:20

... explained as being due to exciton splitting between the symmetric pairs of [Cd(Scys)4]2 groups in the Cd4(Scys)11 binding site [27] As noncooperative metal binding is predicted to result in the formation ... a domain prior to that of the b domain Although the metal -binding reaction has been shown to proceed noncooperatively, this does not mean that a distinct order of metal binding to each of the ... view of metal binding to MT and have been cited regularly in recent reports The data presented in these papers clearly show domain-specific binding for M2+ (M ¼ Zn, Cd) initially to the a domain,...

Ngày tải lên: 19/02/2014, 00:20

... buffer, and n is the net number of protons transferred during binding To investigate whether there is a net proton transfer on IL-8 binding to the receptor N-domain, ITC measurements were carried ... IL-8 binding to its receptor CXCR1 N-domain has been characterized using ITC This report describes how different enthalpic and entropic factors could mediate chemokine binding to its receptor ... in binding may be due to binding of N-loop residues to the receptor N-domain The structure of IL-8 is known, and the structural basis for its function has been well studied [8–17] The receptor...

Ngày tải lên: 19/02/2014, 05:20

... Histograms compare the aminoacylation of 0.33 lM tRNAs: 1, M maripaludis tRNASer; 2, M maripaludis tRNASec; 3, M jannaschii tRNASer; 4, M jannaschii tRNASec; 5, E coli tRNASer; 6, S cerevisiae tRNASer ... helical tRNASer acceptor stem by E coli seryl -tRNA synthetase EMBO J 15, 2843–2849 29 Dock-Bregeon, A.-C., Garcia, A., Giege, R & Moras, D (1990) The contacts of yeast tRNASer with seryl -tRNA synthetase ... maripaludis tRNASerGCU , M jannaschii tRNASerGCU , M maripaludis tRNASecUCA and M jannaschii tRNASecUCA tRNA transcripts were purified by electrophoresis on denaturing polyacrylamide gels Full-length tRNAs...

Ngày tải lên: 19/02/2014, 12:20

... contribute to high-affinity binding of the ephrin B cytoplasmic domain to the Grb4 adaptor protein [11] The equivalent Tyr304 residue of Xenopus ephrin B1 was shown to be essential for binding to Grb4 ... peptide are generated to determine the binding specificity of the Grb4 SH2 in relation to the closest consensus binding sequence of the Src SH2 domain (i.e pYEEI) responded to the addition of the ... essentially the same binding affinities to the GST-PDZ protein in agreement with previous observations [8] Binding experiments with the isolated PDZ domain showed similar binding affinities to the ephrin...

Ngày tải lên: 19/02/2014, 12:20

... concentrated stock solution (% 30 mgÆmL)1) using a Hamilton (Reno, NV, USA) analytical micro syringe An equal volume of the protein was added to the reference cell, to correct for any contribution to the ... indicate that binding of these porphyrins to MCL is governed by enthalpic forces and that the entropic contribution to the binding process is negative The enthalpy and entropy of binding for the ... observed when the same porphyrin binds to jacalin [52] The CD studies presented here suggest that porphyrin binding to MCL is probably similar to porphyrin binding by ConA, and most likely involves...

Ngày tải lên: 19/02/2014, 16:20

... bottom of the autoradiograph (20 pM) was incubated without (–) (lane 1) and with 10 pM Hfq-His6 (+) (lanes 2–7) and increasing amounts of the competitor RNA indicated at the top of the autoradiograph; ... FEBS M Folichon et al A Hfq binding to RNA stimulates elongation by PAP I B Fig A monophosphate 5¢ end stimulates polyadenylation but not Hfq binding (A) Hfq binding to mono- and tri-phosphorylated ... transfered to room temperature Hfq was eluted with Buffer B plus m urea and dialyzed at °C into Buffer A with 0.5 mm dithiothreitol Purified Hfq was stored at °C in the same buffer containing 0.1% Triton...

Ngày tải lên: 19/02/2014, 16:20

... constants K MS1 to K MS5 have been introduced in the model to take into account subtraction of myristate by additional binding sites no to essentially uncoupled to FA1 and ⁄ or FAx Fatty acid binding ... 4672–4683 ª 2005 FEBS Myristate and Mn(III)heme binding to HSA–heme mation from the N to the B state) is very similar to that induced by myristate binding to site FAx Indeed, the HSA affinity for Mn(III)heme ... established by FA binding to domain III (i.e to FA4 and FA5) [26,37–39] On the other hand, myristate bound at the limit of subdomain IA (i.e to FA2) was suggested to be functionally linked to Sudlow’s...

Ngày tải lên: 20/02/2014, 02:22

... that could mediate binding to CD11b and probably also to CD11a CD11b I domain inhibits red cell binding to purified integrin Red cells bind poorly to CD11a/CD18 but more efficiently to CD11b/CD18 [28] ... transfectants to both I domains CaCl2 alone was not sufficient to support the maximal binding of any of the ICAM transfectants to the I domain fusion proteins Ó FEBS 2003 Fig Binding of red cells to CD11a/CD18 ... ICAM-4 transfectants to both I domains was efficiently, but not totally abolished, as was also the binding of ICAM-1 transfectants to the CD11b I domain fusion protein The inhibitory effects of EDTA...

Ngày tải lên: 20/02/2014, 11:20

... Palom, Y., He, Q.Y & Tomasz, M (2001) Selective activation of mitomycin A by thiols to form DNA crosslinks and monoadducts: biochemical basis for the modulation of mitomycin cytotoxicity by the quinone ... the GSH, the cytotoxic effect was slightly reduced, by a factor of about three The 50% inhibitory concentration (IC50) value of 2.2 lM measured with S23906-1 alone was increased to 7.1 lM in the ... alkylator, mechlorethamine hydrochloride, we observed a reduction of cytotoxicity by a factor of about six In this case, the IC50 value of 4.2 lM determined with mechlorethamine alone increased to...

Ngày tải lên: 20/02/2014, 11:20

... induced by cAMP binding to the anti-cAMP -binding sites of CRP, which in turn trigger specic pathways of signal transmission from the cyclic nucleotide -binding domain to the DNA -binding domain ... binding to the syn-cAMP -binding sites at concentration of the ligand of mM causes a % 6% increase in its uorescence intensity, which indicates that this residue is sensitive to cAMP binding to ... that binding in solution of two cAMP molecules to highafnity anti-cAMP -binding sites at the N-terminal domain causes the C-terminal domain to shift further to the N-terminal domain of CRP To conrm...

Ngày tải lên: 20/02/2014, 23:20

... blood of normal donors These cells were not sensitive to the cytotoxic effect of GT oligomers Ó FEBS 2003 eEF1A binding to aptameric cytotoxic GT oligomers (Eur J Biochem 270) 3257 Fig SouthWestern ... growth-inhibition effect exerted by cytotoxic GT oligomers and their selective binding to nuclear eEF1A In fact, in normal human lymphocytes no appreciable binding of GT oligomer to nuclear eEF1A was shown ... corresponding to the N-terminal region of eEF1A, were present; this region is totally deleted in PTI-1 Similar considerations were taken into account to exclude the possibility that P1 corresponded to isoforms...

Ngày tải lên: 21/02/2014, 00:20

... during vectorial transfer of tRNA/ aminoacyl -tRNA in the eukaryotic elongation cycle [13]: [ribosomal E site tRNA] (1) [eEF1AặGDP tRNA] (2) [eEF1AặGDP tRNA] ặARS (3) [eEF1AặGTPặaminoacyl -tRNA] (4) ... intensity before tRNA addition, I tRNA is the ? intensity at given tRNA concentration Data were curvetted by nonlinear least squares to a bimolecular binding isotherm according to the expression: ... of tRNA as well (Fig 6) It means that tRNA binding is not critically important for the quaternary complex formation However, tRNAPhe accelerates the association phase of eEF1AặGDP binding to...

Ngày tải lên: 21/02/2014, 15:20

... corresponded to that obtained in the absence of tHSAheme-Fe(III) (l0 = 2.4 ã 10)1 s)1) Ibuprofen binding to tHSAheme-Fe(II)-NO Figure shows the binding isotherm for ibuprofen binding to tHSAheme-Fe(II)-NO ... at the heme -binding pocket (FA1) Indeed, ibuprofen binding to HSAheme has been reported to induce the hexa-coordination of the heme Fe atom [18,21,23] Thus, peroxynitrite cannot bind to the heme ... according to Eqn (4) [21,32,38,39]: lon ẳ lontopị flontopị ẵibuprofenị=K3 ỵ ẵibuprofenịg 4ị where lon(top) represents the asymptotic value of lon under conditions where [ibuprofen] = (i.e lon(top)...

Ngày tải lên: 06/03/2014, 01:20