DNA and chromosomes

. Histones Chromosomal DNA and its Packaging Human Chromosome Complex of DNA and protein is called chromatin 44 homologous chromosomes and 2 sex chromosomes Complementary DNA with different Dyes The. cycle Mitotic and interphase chromosome Single chromosome can only be visible during mitosis Chromosomes at interphase and M phase • DNA and Chromosomes Three important DNA sequences Telo...

Ngày tải lên: 13/03/2014, 16:39

... results from chromosomal DNA damage caused by the DNA- nicking and DNA- attaching activities of NS1. Upon testing this hypothesis, we found that NS1 covalently binds to cellular DNA and is modified ... suggestive of DNA damage as a cause of NS1-induced apoptosis. ATM principally binds to free DNA ends or DNA strand breaks (43), while ATR recognizes single-stranded regions of...

Ngày tải lên: 25/10/2012, 11:18

. in Bangkok, Thailand. Subjects with and without HAD were recruited and matched for age, gender, education, and CD4 cell count. PBMC and cellular subsets were analyzed for HIV DNA using real-time. clades A, E, and B; and demonstrated equivalent amplification of the target gene. Separation of PBMC Subsets and HIV DNA Analay- sis. Because HIV DNA is present in both lympho- cytes and monocytes,...

Ngày tải lên: 31/10/2012, 15:34

. ETS-1 (lanes 3 and 4) or ssDNA L (lanes 1 and 2). (B) EMSA was per- formed with EWS (lanes 2, 4 and 6) and 32 P-labeled Htelo (lanes 3 and 4), dsHtelo (lanes 5 and 6) or ssDNA S (lanes 1 and 2). The structures. (lanes 2 and 4) and 32 P-labeled Htelo (lanes 1 and 2) or rHtelo (lanes 3 and 4). (B) EMSA was performed with RGG3 (lanes 2 and 4) and 32 P-labeled rHtelo (lanes 1 and 2) or mut rHtel...

Ngày tải lên: 15/02/2014, 01:20

. intensity was  45% of its initial abundance. DNA strand break analysis The ability of Cu(II)-Ami to induce strand breaks in plasmid DNA in the absence and presence of H 2 O 2 was tested with the. concentration effect of Ami between 5 l M and 25 m M , and differences in the extent of formation of form II between experiments (compare lanes 5 and 8 in Fig.4Aandlanes 5and9 inFig.4BwiththoseinFig....

Ngày tải lên: 21/02/2014, 01:21

. properties for DNA/ DNA and RNA /DNA duplex formation Peng Wu 1, *, Shu-ichi Nakano 1 and Naoki Sugimoto 1,2 1 High Technology Research Center and 2 Department of Chemistry, Faculty of Science and Engineering,. para- meters of the 24 DNA/ DNA and 41 RNA /DNA duplexes at standard temperature (25 °C) and physiological temperature (37 °C). Direct comparison shows that the temperature-independent...

Ngày tải lên: 22/02/2014, 07:20

. one strand and only the 3¢-end G-tract of the other strand [30] (Fig. 3D). The core of this structure, called the (3 + 1) core, has three strands oriented in one direction and one strand oriented. consecutive G-tracts are 5¢-syn-anti-anti-3¢ and 5¢-syn-syn-anti-3¢ for one strand and 5¢-syn-syn-anti-3¢ and 5¢-syn-anti-anti-3¢ for the other strand of the dimer; glycosidic conformations of. of DNA...

Ngày tải lên: 06/03/2014, 09:22

. tail and 3’ blunt end, DnaB enters DNA with both strands passing through the central channel of DnaB (Kaplan, 2000). The translocation of DnaB on double-stranded DNA (dsDNA) requires ATP hydrolysis.. and spatially separate DNA unwinding by DnaB helicase from its ATP-dependent locomotion on DNA (double- or single-stranded). It is known that when encountering a linear DNA with a 5’ tail and....

Ngày tải lên: 06/03/2014, 23:20

. NaH 2 PO 4 , and KI were obtained either from Fluka (Buchs, Switzerland) or from Riedel-de- Hae ¨ n (Seelze GmbH, Germany). dNTP, Pwo DNA poly- merase, HindIII and EcoRI endonucleases, and T4 DNA ligase. isothermal titration calorimetry for lac and gal, respectively [35]. The 26 bp long DNA sequences – lac, gal and ICAP – have CRP conformational changes induced by DNA and cAMP M. Tworzydło et a...

Ngày tải lên: 07/03/2014, 16:20

. 23 chromosomes to her child through her egg and a father passes 23 chromosomes through his sperm The chromosomes are made up of DNA Each chromosome consist of two very long thin strands of DNA. cells because the cells have different roles and make different proteins GENES AND CHROMOSOMES: THE GENOME GENES AND CHROMOSOMES: THE GENOME GENES AND CHROMOSOMES: THE GENOME FACT SHEET FA...

Ngày tải lên: 13/03/2014, 21:58