Báo cáo Y học: An active site homology model of phenylalanine ammonia-lyase from Petroselinum crispum docx

... catalysis. Keywords: phenylalanine ammonia-lyase; PAL; MIO; site- directed mutagenesis; homology model. Phenylalanine ammonia-lyase (PAL; EC 4.3.1.5) is a very important plant enzyme that catalyses ... for Organic Chemistry, University of Karlsruhe, Germany; 2 Institute for Organic Chemistry, Budapest University of Technology and Economics, Hungary The plant enzyme phenylalan...

Ngày tải lên: 08/03/2014, 23:20

... Germany) and diethylpyrocarbonate (DEPC) was from AppliChem (Darmstadt, Germany). All solvents and chemicals were of analytical grade and obtained from Merck (Darmstadt, Germany), Sigma (Deisenhofen, ... member of the a/b hydrolase enzyme superfamily. Keywords: polyneuridine aldehyde esterase; active- site resi- dues; site- directed mutagenesis; modelling; a/b hydrolase enzyme sup...

Ngày tải lên: 24/03/2014, 04:21

... to the active site [23]. Zymogen conversion to the active enzyme occurs by limited proteolysis of the inhibitory pro-sequence segment and may be either autocatalytic or involve acces- sory molecules. ... of propeptide may vary and range from short polypeptide segments to independently folded domains comprising more than 100 residues [24,25]. Often the precise length of the matur...

Ngày tải lên: 08/03/2014, 16:20

... characterization and inhibitor discovery of shikimate dehydrogenase from Helicobacter pylori Cong Han 1 , Lirui Wang 1 , Kunqian Yu 1 , Lili Chen 1 , Lihong Hu 1 , Kaixian Chen 1 , Hualiang Jiang 1,2 and ... Discovery and Design Center, State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, China 2 School of Pharmacy, Ea...

Ngày tải lên: 19/02/2014, 05:20

... very important and patients who have had a previous attack of gout should be made aware of common signs and symptoms, treatment protocols during an acute attack, and that gout may occur in any ... and symptoms of an acute gouty attack and be made aware that changes in certain medication dosages may precipitate an attack. Awareness of radiographic changes associated with gout is...

Ngày tải lên: 25/10/2012, 10:06

... immune system is concerned. Type A infects pigs, horses, seals, whales, and many types of birds as well as humans. This can be a trans-species virus. Type B infects only humans [8]. Animals ... eventually infect humans. Supporting Shope’s hypothesis of trans-species infection an incident occurred in an unrelated study in 1928, of canine distemper, with ferrets being used as th...

Ngày tải lên: 02/11/2012, 11:12

... timely study. Microbial genome sequencing is likely to play an increasingly important role in the analysis of newly discovered human genes and provide further clues to the molecular basis of the ... also urinary levels of pyridinolines and deoxypyridinolines and serum and urine levels of type 1 collagen telopeptides (CTX and NTX), which are indices of bone resorption. The level...

Ngày tải lên: 03/11/2012, 09:54

... commonly-used suture threads, namely polyurethane, polypropylene, and polyamide. Materials and methods: The mechanical properties of same-size suture threads made from polyurethane, poly- propylene, ... glycolide and epsilopn caprolacton copolymer monofilament 50% at 14 days 105 days good Obstetrics- gynecology, urology, plastic, abdominal, and vascular POLYDIOXANONE: ester polymer...

Ngày tải lên: 03/11/2012, 11:52

... putrescine and higher polyamines [1] and may have important regulatory roles in mammals [3–5]. Agmatinases from Escherichia coli and human tissues, and putative agmatinases from Synechocystic sp. ... decreased activity of a D153N variant of E. coli agmatinase. From the enzymic properties of D153N agmatinase and a modelled structure, we conclude that the lower activity of the mut...

Ngày tải lên: 21/02/2014, 01:21

... separate control (noncrystalline) areas, a redundancy of > 4 or > 5 would arise by chance with a p robability (see below) of only 0.022 and 0.004, respectively, for any given structure factor. ... number of observations of a structure factor in a data set of n crystals, with p being the individual (one-off) probability of observing data of IQ 7 o r better by chan ce alone....

Ngày tải lên: 17/03/2014, 17:20