Báo cáo Y học: Kinetic analysis of hydroxylation of saturated fatty acids by recombinant P450foxy produced by an Escherichia coli expression system docx

... Kinetic analysis of hydroxylation of saturated fatty acids by recombinant P450foxy produced by an Escherichia coli expression system 7 Tatsuya 7 Kitazume 1 , Akinori Tanaka 1 , Naoki Takaya 1 , ... catalyzes the subterminal (x-1x-3) hydroxylation of fatty acids. Here, we produced, purified and characterized a fused recombin- ant protein (rP450foxy)...

Ngày tải lên: 08/03/2014, 22:20

... JNK, and phosphorylated JNK, although expression of PTEN and Bcl-X did not change. Bag-1 may inhibit apoptosis by suppressing the expression of Bid and Bad. It may also enhance apoptosis by inhibiting ... trend of protein changes for the experimental sample to control detected by using the Odyssey Infrared Imaging System. (+) means an increase of signal intensity, and (...

Ngày tải lên: 31/10/2012, 15:12

... Department of Biochemistry of the Charité, Berlin, Germany using the ABI PRISM Big Dye Terminator system (Applied Biosystems, Germany). Sequences were analyzed using the Chromas software (Technely- sium ... Results Mutational analysis of hCDC4 in AML In this study the common exons 2 to 11 and the three known transcript variants of exon 1 of hCDC4 (Figure 1A) were analyzed...

Ngày tải lên: 31/10/2012, 16:49

... Expression profile of the most sig- nificantly changed genes with age. This profile was generated in the NHGRI website (https://arrayanalysis.ni h.gov), using dis- tance-based analysis of ... extraction and array analysis. For each array sample, the RNA was prepared from the purified thymocytes. The thymocytes were processed by using the RNEasy Mini Kit (Qiagen, Valencia, C...

Ngày tải lên: 03/11/2012, 11:35

... hydrolysis of o-NTFNAC was recorded at 430 nm (e = 3954 m )1 Æcm )1 ) for 5 min. Acetyl esters Kinetic analysis of the BuChE-catalysed hydrolysis of o-NPA was followed by monitoring the rate of ... Kinetic analysis of butyrylcho- linesterase-catalyzed hydrolysis of acetanilides. Biochim Biophys Acta 1774, 1139–1147. 36 Tsujita T & Okuda H (1983) Carboxylesterases in...

Ngày tải lên: 18/02/2014, 17:20

... equimolecular amount of DM43 to the enzymes. Statistical analysis Results represent mean ± SEM (n ‡ 4). Data were statis- tically evaluated by Analysis of Variance ( ANOVA ), followed by Newman-Keuls-Student’s ... potentiated by enzymatic degradation of phospholipids [7,44]. The capacity of DM64 to inhibit the myotoxicity induced by myotoxins I (Asp49) and II (Lys49) from...

Ngày tải lên: 21/02/2014, 01:21

... slightly larger in size than these produced by recombinant p 26. As revealed by sedimentation patterns therefore truncated p26 variants detectable by antip26 antibody exhibited limited ability t ... ordinary hydration and temperature, cell maintenance entails a constant and substantial free energy flow [51,52]. Anoxic cysts m ay acquire s ufficient energy to survive b y utilization...

Ngày tải lên: 22/02/2014, 04:20

... purified by TLC chromatography [16] and its concentration was measured by the phosphate analysis [17]. Glyceraldehyde phosphate, dihydroxyacetone phosphate, sn-glycerol-2-phosphate, and dihydroxyacetone ... reduction by NAD + at 2.1 m M dihydroxyacetone phosphate and varying NADH concentration; B-2, in hibitio n of dihydroxyacetone phosphate reduction by NAD + at 0.32 m M NADH and...

Ngày tải lên: 22/02/2014, 04:20

... 25 °C. Determination of kinetic parameters All kinetic constants and corresponding asymptotic stand- ard errors we re determined by nonlinear r egression analysis of the untransformed data using ... baculo- virus expression system. The recombinant enzyme was purified to app arent homogeneity b y poly(ethylene glycol) precipitation, followed by chromatography on DEAE– 1270...

Ngày tải lên: 22/02/2014, 04:20

... been clarified yet, and the possibility of in vivo inhibition of the enzymatic activity of tTG by CD autoantibodies should be carefully considered. CD is frequently accompanied by other autoimmune ... amino acids (amino acids 140–376), as proved by the reactivity of tTG/11, tTG/7, which overlap by this region only. However, this conclusion is slightly complicated by the l...

Ngày tải lên: 08/03/2014, 09:20