Báo cáo Y học: Identification of residues in the PXR ligand binding domain critical for species specific and constitutive activation docx

... of the human and mouse PXR ligand binding domains. By modeling the human PXR ligand binding domain we have identified and mutated two polar residues in the puta- tive ligand binding pocket which ... built a homology model of human PXR in order to identify residues that were likely to be involved in ligand binding. Since the initiation of this...

Ngày tải lên: 08/03/2014, 16:20

... C a of Glu and its carboxylate oxygen, C a of Asp a nd its carboxylate oxygen, C a of Arg and its side-chain amines or guanidinium, and C a of Lys and its side-chain amine was determined by measuring ... of these residues were clustered into nine regions of identity (I–IX). Inspec- tion of these regions showed that residues implicated in Zn 2+ binding and catal...

Ngày tải lên: 08/03/2014, 23:20

... similarity to type III PKSs, which lack this domain, is scant. This domain promotes the binding of the acyl-CoA initiation unit to the ketosynthetase domain of the PKSs for polyketide biosynthesis ... genes of the A201A biosyn- thetic pathway, may be related to their counterparts of the puromycin and hygromycin A biosynthetic pathways, respectively. The puromy...

Ngày tải lên: 21/02/2014, 01:21

... s erine/threonine protein k inases widely expressed in a range of eukaryotes including yeast, mammals and plants. They have been shown to play a role in diverse physiological events including ... CKI may play a role in synaptic vesicle exocytosis. Keywords: CKI; CKII; syntaxin-1A; trafficking. Casein kinase I (CKI) belongs to a family of serine/ threonine protein kinases with seven...

Ngày tải lên: 22/02/2014, 04:20

... Saccharomyces cerevisiae has four such MIP channels: the aquaporins Aqy1 and Aqy2 and the aquaglyceroporins Fps1 and Y 054 [8]. Aqy1 is a strictly spore -specific aquaporin while Aqy2 may play a role in ... screen for hyperactive Fps1 and isolated mutations in 14 distinct resi- dues, all facing the inside of the cell. Five of the residues lie within the previo...

Ngày tải lên: 07/03/2014, 15:20

... Rpn8 and Rpn11), and described the relationship between N-acetylation and the chymotrypsin-like activity of 20S proteasome. In the present s tudy, we detec ted the N-acetylation of OsRpt6 in the ... 269) 1475 Protein assay The protein concentration of the sample was d etermined by the Bradford method [17] using c-globulin as a standard. Western blotting analysi...

Ngày tải lên: 08/03/2014, 16:20

... four membrane-associated domains. These four domains are organized as a cluster of three closely spaced domains near the centre of the polypeptide, and a fourth well separated domain close to the C-terminal end of the ... designed for the acetylcholine (ACh) and glycine receptors of the cys-loop receptor superfamily. The key feature was a closely spaced triplet...

Ngày tải lên: 17/03/2014, 23:20

... 2002 terminus that is required for binding to the plasma membrane, thereby being involved in the formation of the corneum [47]. Additional amino-terminal residues of TGase 1 include the sequences ... catalyses cross- linking between the c-carboxyamide of glutamine residues and the e-amino group of lysine residues or other primary amine. The reaction leads t...

Ngày tải lên: 17/03/2014, 23:20

... However, the limitation of the kinetic method for determination of the number of amino-acid residue(s) and also of the stoichiometry of the reaction was indicated by Levy et al. [22]. Therefore the ... value of 9.0 due to rapid loss of the enzyme activity of the control sample. It was observed that for the EAC cell enzyme, the rate of inactivation w...

Ngày tải lên: 24/03/2014, 04:21

... glycerol, 0.8 m M NaP i ,4mM spermidine, and 3 mg poly(dI : dC). Where indicated, 5 pmol unlabelled probe was included in the binding reaction. Binding was performed for 15 min, at this point ... phosphorylated by the intrinsic Ser/ Thr-kinase activity of the activated receptor. Once phosphorylated, Smads 2/3 bind to the ‘common pathway’ Smad 4 followed by translocation o...

Ngày tải lên: 31/03/2014, 23:20