Báo cáo Y học: Identification and characterization of a new gene from Variovorax paradoxus Iso1 encoding N -acyl-D-amino acid amidohydrolase responsible for D-amino acid production pdf
Báo cáo Y học: Identification and characterization of a new gene from Variovorax paradoxus Iso1 encoding N -acyl-D-amino acid amidohydrolase responsible for D-amino acid production pdf
... higher hydrolysing activity against N- ace-tyl-D-amino acid derivates containingD-methionine,D-leucine and D-alanine and against N- chloroacetyl-D-phe-nylalanine. Importantly, the enzyme ... Alcaligenes xylosoxydans ssp.xylosoxydans A- 6 N- acyl-D-Asparate amidohydrolase; A- 6-D50061: Alcaligenesxylosoxydans ssp. xylosoxydans A- 6 N- acyl-D-glutamate amidohydrolase; Alicaligenesfaecalis-DA1: ... Alicaligenesfaecalis-DA1: Alcaligenes faecalis DA1 N- acyl–D-amino acid amidohydrolase; V. paradoxus Iso1: Variovorax paradoxus Iso1 N- acyl-D-amino acid amidohydrolase; P. abyssi: Pyrococcus abyssi N- acyl-D-aminoacid...
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Tài liệu Báo cáo Y học: Identification and characterization of a mammalian 14-kDa phosphohistidine phosphatase pdf
... fluorophosphate was from FlukaChemie AG, Switzerland. Phenylmethanesulfonyl fluoride and okadaic acid were from Sigma. Trypsin of modifiedsequencing grade was obtained from Promega, and LysC of Achromobacter ... phosphatase and anendosomal protein may be of interest in the light of therecently described histidine phosphorylation of annexin I from a membrane preparation of ovine tracheal epithelia[34]. Although ... proteins. Theamino acid sequence of human phosphohis-tidine phosphatase (Fig. 1) was used as thequery in a search against translated se-quences of nonredundant nucleotide and EST databases at...
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Báo cáo Y học: Identification and characterization of a novel activated RhoB binding protein containing a PDZ domain whose expression is specifically modulated in thyroid cells by cAMP pot
... by UV radiation and DNA damaging and by its ability to regulate NFjBdependent transcription [9,10].Many efforts have been focused on elucidation of Rhosignaling events and recent studies have ... play a key role betweenRhoB and potential downstream elements needed understimulation of the thyrotropin/cAMP pathway in thyrocytes and responsible for intracellular motile phenomena such asthe ... dominantnegative and pcDNA3.myc–RhoBQ63L constitutively act-ive were created [8]. RhoA and Rac1 wt, dominant negative and constitutively active cDNAs were kindly provided byM. Spaargaren (Utrecht...
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Tài liệu Báo cáo Y học: Identification and characterization of the Escherichia coli stress protein UP12, a putative in vivo substrate of GroEL pptx
... temperature in SOCmedium and then plated on Luria–Bertani agar platescontaining kanamycin. Kanamycin resistant (KmR)trans-formants were selected and colony-purified on agar platesincubated ... regions adjacent to thebeginning and the end of ybdQ and additional 20-nucleotidecomplementary to the template plasmid pKD13 carryingthe kanamycin resistance (kan) gene. The resulted linear1.4-kb ... aremolecular chaperones. Functionally, they bind to non-nativestructural forms of various polypeptides and assist them inreaching a native conformation [19]. Consequently, asmolecular chaperones,...
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Báo cáo khoa học: Identification and characterization of a nuclear receptor subfamily I member in the Platyhelminth Schistosoma mansoni (SmNR1) pot
... transcription in a mammalian cellreporter gene assay in in vivo results.Experimental proceduresParasitesThe NMRI strain of S. mansoni was maintained in snails(Biomphalaria glabrata) and Syrian ... eggs,sporocysts and 21-day worms.Determination of transactivation A yeast one-hybrid assay was employed to determinewhether ligand-independent autonomous transactiva-tion function was present in SmNR1. ... was determined by competi- A BFig. 6. Yeast one and two-hybrid assays. (A) Yeast one hybridassay showing that SmNR1 contains an autonomous transactivationfunction in A ⁄ B domain. Individual...
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Báo cáo khoa học: Identification and characterization of 1-Cys peroxiredoxin from Sulfolobus solfataricus and its involvement in the response to oxidative stress pdf
... in addition ROS can begenerated by naturally occurring phenomena such asultraviolet irradiation of water, autoxidations and aeration turbulence. Consequently, to survive in thisharsh habitat ... thiol-specificantioxidant from mammalian brain: alkyl hydroperoxidereductase and thiol-specific antioxidant define a largefamily of antioxidant enzymes. Proc Natl Acad Sci USA91, 7017–7021.6 Chae HZ, ... this enzyme acts as a catalyst in H2O2reduction and protects plasmid DNA from nicking bythe metal-catalysed oxidation system. Western blot analysis revealed thatthe Bpc2 expression was induced...
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Báo cáo khoa học: Identification and characterization of a collagen-induced platelet aggregation inhibitor, triplatin, from salivary glands of the assassin bug, Triatoma infestans ppt
... pres-ence or absence of 1.0 lM triplatin-1 and analyzed by anti-FcR c-chain(aFcR c-chain) and antiphosphotyrosine (aPY) immunoblotting. A. Morita et al. Identification and characterization of triplatinFEBS ... respectively.Fig. 2. Western blot analysis of triplatin-1 and -2 from the salivarygland of T. infestans. Extracts from a pair of salivary glands, recom-binant triplatin-1 and recombinant triplatin-2 ... Identification and characterization of a collagen-inducedplatelet aggregation inhibitor, triplatin, from salivaryglands of the assassin bug, Triatoma infestansAkihiro Morita1, Haruhiko...
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Báo cáo Y học: Purification and characterization of the thyrotropin-releasing hormone (TRH)-degrading serum enzyme and its identification as a product of liver origin doc
... human; L in rat), 680 (L in pig and human; I in rat), 760 (K in pig; R in rat and human), 991 (A in pig and human; S in rat), 1009 (M in pig and human; R in r at) and 1023 (L in pig and hu man; ... structure and intercellular granula, increase of connective tissue and appearance of noduli and fibrotic septa were noticed (datanot shown).Synthesis of the TRH-DE by hepatocytes in cultureIn contrast ... (1990)Regulation and cellular localization of the membrane-boundthyrotropin-releasing h ormone-degrading enzyme in primarycultures of neur onal, glial and aden ohypophyseal cells.Endocrinology 127...
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Tài liệu Báo cáo khoa học: Identification and characterization of the transcription factors involved in T-cell development, t-bet, stat6 and foxp3, within the zebrafish, Danio rerio docx
... domain of t-bet, the STAT protein inter-action domain, STAT protein all-alpha domain, STATprotein DNA-binding domain and SH2 domain of stat6, and the zinc-finger domain, leucine-zipperdomain and ... 27–37.14 Yamashita M, Ukai-Tadenuma M, Miyamoto T,Sugaya K, Hosokawa H, Hasegawa A, Kimura M,Taniguchi M, DeGregori J & Nakayama T (2004)Essential role of GATA3 for the maintenance of type ... human and zebrafish regionscontaining the stat6 gene, showing that the use of a synteny approach to look for genes may not work inall cases. Studies looking at the Fugu and humangenomes have...
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Tài liệu Báo cáo khoa học: Identification and characterization of an R-Smad ortholog (SmSmad1B) from Schistosoma mansoni pdf
... Regions encoding MH1, linker and MH2 are in light gray, gray and dark gray, respectively, and the regions representing the 5¢- and 3¢-UTRs areshown in white in the genomic gene and the cDNA. Intron size ... interactionsYeast two-hybrid and three-hybrid assaysThe yeast strain Y1 90, a HIS3 ⁄ LacZ reporter strain, wasutilized in the yeast two-hybrid assays. Preparation of yeastcompetent cells and ... short linker region comprising only 42 aminoacids. The N- terminal MH1 domain of SmSmad1Bconsists of 137 amino acids, and comprises a conservednuclear localization signal and a b-hairpin structurethat...
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