Báo cáo Y học: Identification and characterization of a new gene from Variovorax paradoxus Iso1 encoding N -acyl-D-amino acid amidohydrolase responsible for D-amino acid production pdf

... higher hydrolysing activity against N- ace- tyl- D -amino acid derivates containing D -methionine, D -leucine and D -alanine and against N- chloroacetyl- D -phe- nylalanine. Importantly, the enzyme ... Alcaligenes xylosoxydans ssp. xylosoxydans A- 6 N- acyl- D -Asparate amidohydrolase; A- 6-D50061: Alcaligenes xylosoxydans ssp. xylosoxydans A- 6 N- acyl- D -glutamate amidohydro...

Ngày tải lên: 08/03/2014, 16:20

... fluorophosphate was from Fluka Chemie AG, Switzerland. Phenylmethanesulfonyl fluoride and okadaic acid were from Sigma. Trypsin of modified sequencing grade was obtained from Promega, and LysC of Achromobacter ... phosphatase and an endosomal protein may be of interest in the light of the recently described histidine phosphorylation of annexin I from a membrane prepar...

Ngày tải lên: 21/02/2014, 01:21

... by UV radiation and DNA damaging and by its ability to regulate NFjB dependent transcription [9,10]. Many efforts have been focused on elucidation of Rho signaling events and recent studies have ... play a key role between RhoB and potential downstream elements needed under stimulation of the thyrotropin/cAMP pathway in thyrocytes and responsible for intracellular motile p...

Ngày tải lên: 23/03/2014, 21:20

... temperature in SOC medium and then plated on Luria–Bertani agar plates containing kanamycin. Kanamycin resistant (Km R )trans- formants were selected and colony-purified on agar plates incubated ... regions adjacent to the beginning and the end of ybdQ and additional 20-nucleotide complementary to the template plasmid pKD13 carrying the kanamycin resistance (kan) gene. The resulted...

Ngày tải lên: 22/02/2014, 07:20

... transcription in a mammalian cell reporter gene assay in in vivo results. Experimental procedures Parasites The NMRI strain of S. mansoni was maintained in snails (Biomphalaria glabrata) and Syrian ... eggs, sporocysts and 21-day worms. Determination of transactivation A yeast one-hybrid assay was employed to determine whether ligand-independent autonomous transactiva- tion func...

Ngày tải lên: 07/03/2014, 11:20

... in addition ROS can be generated by naturally occurring phenomena such as ultraviolet irradiation of water, autoxidations and aeration turbulence. Consequently, to survive in this harsh habitat ... thiol-specific antioxidant from mammalian brain: alkyl hydroperoxide reductase and thiol-specific antioxidant define a large family of antioxidant enzymes. Proc Natl Acad Sci USA 91, 7017–...

Ngày tải lên: 07/03/2014, 12:20

... pres- ence or absence of 1.0 l M triplatin-1 and analyzed by anti-FcR c-chain (aFcR c-chain) and antiphosphotyrosine (aPY) immunoblotting. A. Morita et al. Identification and characterization of triplatin FEBS ... respectively. Fig. 2. Western blot analysis of triplatin-1 and -2 from the salivary gland of T. infestans. Extracts from a pair of salivary glands, recom...

Ngày tải lên: 30/03/2014, 10:20

... human; L in rat), 680 (L in pig and human; I in rat), 760 (K in pig; R in rat and human), 991 (A in pig and human; S in rat), 1009 (M in pig and human; R in r at) and 1023 (L in pig and hu man; ... structure and intercellular granula, increase of connective tissue and appearance of noduli and fibrotic septa were noticed (data not shown). Synthesis of the TRH-DE by hepa...

Ngày tải lên: 31/03/2014, 21:21

... domain of t-bet, the STAT protein inter- action domain, STAT protein all-alpha domain, STAT protein DNA-binding domain and SH2 domain of stat6, and the zinc-finger domain, leucine-zipper domain and ... 27–37. 14 Yamashita M, Ukai-Tadenuma M, Miyamoto T, Sugaya K, Hosokawa H, Hasegawa A, Kimura M, Taniguchi M, DeGregori J & Nakayama T (2004) Essential role of GATA3 for the ma...

Ngày tải lên: 16/02/2014, 09:20

... Regions encoding MH1, linker and MH2 are in light gray, gray and dark gray, respectively, and the regions representing the 5¢- and 3¢-UTRs are shown in white in the genomic gene and the cDNA. Intron size ... interactions Yeast two-hybrid and three-hybrid assays The yeast strain Y1 90, a HIS3 ⁄ LacZ reporter strain, was utilized in the yeast two-hybrid assays. Preparation o...

Ngày tải lên: 18/02/2014, 16:20