... stability, and kinetic parameters of C5a peptidase
from
Streptococcus pyogenes
Elizabeth T. Anderson
1
, Michael G. Wetherell
1
, Laurie A. Winter
1
, Stephen B. Olmsted
1
,
Patrick P. Cleary
2
and Yury ... not
available for C5a peptidase from pathogenic Streptococcus
pyogenes. One of the aims of this study was to map the pro-
sequence region of C5a pep...
... macrocarpa), hyoscyamine 6b-hydroxylase
from Hyoscyamus niger, the iron-deficiency-specific proteins 2 and 3 from Hordeum vulgare; desacetoxyvindoline-4-hydroxylase from Catharanthus
roseus, 11 aminocyclopropane-1-carboxylic-acid ... formed from 4-coumaroyl-
CoA and malonyl-CoAs by the action of
chalcone synthase and chalcone isomerase, is
3b-hydroxylated by flavanone 3b-hydroxyl...
... over the years in
understanding the behavior and clinical course of the disease; however, the
natural history of HBV is complex and we still have much to explore and
learn.
K
K
e
e
y
y
w
w
o
o
r
r
d
d
s
s
... Pan
1
and Jin X. Zhang
2
1
Division of Gastroenterology and Hepatology, Elmhurst Hospital Center of Mount Sinai
Services, Mount Sinai School of Medici...
... Once oocysts are shed, they re-
quire 1 to 5 days to sporulate and become infective.
Cats pass oocysts for only 2 to 3 weeks following
primary infection. Mature cats are less likely to shed ... represent any risk for
infection with Toxoplasma gondii.
Cats are the definitive host of T. gondii; they are
the only animals that pass oocysts in their feces. They
become infected by eating...
... production of cystatin F compared
to the ubiquitous inhibitor, cystatin C, we measured the
cystatin contents in cell lysates and culture media of cells
grown for 2 and 4 days with cystatin F and cystatin ... inhibitor, cystatin C. Cystatin F purified
from lysates of U937 cells showed three major forms car-
rying two, one or no carbohydrate chains. Immunocyto-
chemistry demonstrat...
... Henkler
2
,
Ottmar Janssen
3
and Harald Wajant
1
1
Institute of Cell Biology and Immunology and
2
Institute of Industrial Genetics, University of Stuttgart, Germany;
3
Institute of
Immunology, Christian-Albrechts-University ... 8).
Correspondingly, fluorescence of cytoplasmic T4(259–470)–
YFP and full-length T4–GFP was already significantly
reduced after 2 min of bleaching...
... purchased were of the highest quality and
purity. Acrylamide, EDTA, cAMP, dithiothreitol, phenyl-
methanesulfonyl fluoride, and Tris were purchased from
Sigma. N-Iodoacetylaminoethyl-1-naphthylamine-5-sulfo-
nate ... protein from Escherichia coli
Magdalena Tworzydło, Agnieszka Polit, Jan Mikołajczak and Zygmunt Wasylewski
Department of Physical Biochemistry, Faculty of Biotechn...
... arro ws indicate the
positions of fully glycosylated (78 kDa) and completely u nglycosylated
(64 kDa) HCII, respectiv ely.
Ó FEBS 2002 Heparin cofactor II: N-glycans and sulfation (Eur. J. Biochem. ... heparin–Sepharose, indicating that tyrosine
sulfation and N-linked glycans may affect the inhibitor’s
interaction w ith g lycosaminoglycans.
Keywords: heparin cofactor II; glycosylation;...
... Purification and biochemical characterization of some
of the properties of recombinant human kynureninase
Harold A. Walsh and Nigel P. Botting
School of Chemistry, University of St Andrews, St Andrews, ... crude and pure (> 95%) forms of the
enzyme. Reproducibility of the experimental findings was
confirmed with enzyme from different batches and varying
degrees of pu...