Báo cáo khoa học: Kinetics of violaxanthin de-epoxidation by violaxanthin de-epoxidase, a xanthophyll cycle enzyme, is regulated by membrane fluidity in model lipid bilayers pptx

... probability that violaxanthin remains violaxanthin; AA, probability that anthera- xanthin remains antheraxanthin; ZZ, probability that zeaxanthin remains zeaxanthin; S VA , the constant rate of ... conversion of violaxanthin to zeaxanthin is catalysed by violaxanthin de-epoxidase (VDE) and the reverse reaction of violaxanthin formation from zeaxanthin is catalysed by an...

Ngày tải lên: 08/03/2014, 10:20

... Methyl a- d-glucopyranoside was used as a glucose analog that is not a substrate of hexokinase in a glucose assay system. The data were analyzed on the basis of the Michaelis–Menten equation; the maximum activity ... GBd, glucan-binding domain; GS, glucan-binding domain-deficient glucosyltransferase-I; GSd, glucansucrase domain; GSGB, glucosyltransferase-I containing a full-le...

Ngày tải lên: 14/02/2014, 22:20

... (PMNs) at the site of in ammation is accompanied by degranulation and/or lysis of these in am- matory cells resulting in the extracellular release of a variety of hydrolases and oxidases, as well as ... porcine pancreatic elastase; Suc- (Ala) 3 -p-NA, succinyl-Ala-Ala-Ala-p-nitroanilide; MeO-Suc-(Ala) 2 - Pro-Val-p-NA, methoxysuccinyl-Ala-Ala-Pro-Val-p-nitroanilide; MeO-Suc-...

Ngày tải lên: 07/03/2014, 15:20

... similar constants for the V s /V h for deacylation with 7-ADCA and 6-APA using PAA and PGA. However they observed in all cases a saturation of the (V s /V h ) max , whereas our data indicate a linear ... increase in activity of PA may be caused by small structural changes in the active site at low concentrations of D 2 O, making enzyme–substrate inter- actions more favourabl...

Ngày tải lên: 08/03/2014, 08:20

... within a multigene family is regulated by transcription factor TFIIIB Akhila Parthasarthy and Karumathil P. Gopinathan Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore, ... DNA bind- ing in gel retardation assays using a labelled fragment containing the TATATAA sequence (Fig. 2D, left). Because TBP is present as a component of TFIIIB, t...

Ngày tải lên: 20/02/2014, 02:21

... semantic analysis, and pragmatic analysis. Each stage has been designed to use linguistic data such as the lexicon and grammar, which are maintained separately from the engine, and can easily ... example, the verb abridge has an associated case frame consisting of an agent doing the abridging and an optional theme that is being abridged. Furthermore, in an English sentence usin...

Ngày tải lên: 20/02/2014, 18:20

... Eda K, Kagawa W, Takata M, Takeda S, Yokoyama S & Shibata T (2001) Homologous-pairing activity of the human DNA-repair proteins Xrcc3.Rad51C. Proc Natl Acad Sci USA 98, 5538–5543. 20 Kagawa ... structure of Rad54 (Protein Data Bank ID: 1Z3I) was obtained from the Protein Data Bank and was employed as a basal structure. A molecular dia- gram was generated using ucsf chimera [48]. A...

Ngày tải lên: 06/03/2014, 09:22

... constructed a pro-tyrosinase mutant carrying an alanine at this position, F45 3A. Curi- ously, an increase in protein expression was obtained for this mutant tyrosinase similar to that obtained when ... tyrosinase constructs without the predicted N-terminal signal peptide (amino acids 1–36), we retained amino acid 36, an alanine, rather than using amino acid 37, a lysine, because it is...

Ngày tải lên: 06/03/2014, 11:20

... promoters indicate the a- F1-ATPase GAF/Adf-1 binding cassette has enhancer properties. (A) The basal promoter activity of b-F1-ATPase is greatly increased when the a- F1-ATPase GAF/Adf-1 binding cassette ... a- F1-ATPase gene is more heavily tran- scribed in the head and thorax of adult flies in comparison to the abdomen (data not shown), indicating that its expression is dif...

Ngày tải lên: 07/03/2014, 16:20

... disease. However, CAA is also seen in normal ageing. There is increasing evidence that CAA may underlie certain forms of vascular dementia and intracranial hemorrhage associated with ageing [1]. ... viability. Ab also caused an increase in the number of apoptotic cells. This increase in apoptosis was accompanied by activation of caspase-3. Homocysteine, a known risk factor...

Ngày tải lên: 22/02/2014, 07:20