Báo cáo khoa học: Identification of microsomal rat liver carboxylesterases and their activity with retinyl palmitate potx

... FEBS 2002 Identification of microsomal rat liver carboxylesterases and their activity with retinyl palmitate Sonal P. Sanghani, Wilhelmina I. Davis, Natividad G. Dumaual, Alan Mahrenholz and William ... and ES3, account for more than 95% of rat liver microsomal carboxylesterase activity. They obey Michaelis– Menten kinetics for hydrolysis of retinyl pa...

Ngày tải lên: 08/03/2014, 10:20

... ovalbumin (43 kDa); and (g) chymotry- psinogen A (25 kDa). Fig. 2. Effect of pH and temperature on Yef1p activity and stability. (A) Effect of pH on ATP-NAD kinase activity. NAD kinase activity was assayed ... kinase. K m for NAD and ATP were 1.9 mm and 0.17 mm, respectively. Properties of Yef1p and identification of Yef1p and Utr1p as ATP-NADH kinases The enzyme h...

Ngày tải lên: 20/02/2014, 01:20

... presence of various amounts of RGG3. Titration of Htelo with RGG3 (1.2, 1.0, 0.8, 0.6, 0.4, 0.2, 0.1 and 0 equiv. of RGG3) in 100 m M KCl and 50 mM Tris ⁄ HCl (pH 7.5). The concentration of DNA ... 2, 4 and 6) was incubated with (lanes 1, 2, 5 and 6) or without (lanes 3 and 4) PRMT3 in a potassium buffer with (lanes 3–6) or without (lanes 1 and 2) AdoMet. (B) Schem...

Ngày tải lên: 15/02/2014, 01:20

... and structural analysis of OSs in LOSs of lpxX and lpxL mutants The OS portion of each LOS was obtained after mild acid hydrolysis and analyzed for its glycosyl composi- tions and by MALDI-TOF ... consis- tent with the conclusion that the OSs from O35ElpxX and O35ElpxL have the same structure as that of O35E. Morphology and growth rate of lpxX and lpxL mutants O35Elp...

Ngày tải lên: 18/02/2014, 14:20

... consisting of a strong short- and a moderate long-term response. Moreover, after 7–10 days of exposure, we detected differential expression of a number of genes that encode elements of the transcription ... HSPs (HSP70 and HSP23) were greatly and rapidly upregu- lated in gills but slightly less and later in mantle. One inducible and two constitutive isoforms of HSP70 are...

Ngày tải lên: 18/02/2014, 16:20

... found to have a molecular mass of 64 kDa, and to contain two tandem repeats and a Glu-rich region. The structure of the protein and that of its DNA are similar to those of starmaker, a protein involved in ... aggregates of proteins and polysaccharides, and may play important roles in formation of the phases and ⁄ or morphologies of the crystals because they consist...

Ngày tải lên: 18/02/2014, 17:20

... suspen- ded in 25 mL of 40% potassium hydroxide and 25 mL of methanol and refluxed for 2 h. Products were extracted with 50 mL of hexane. The hexane layer was washed with 25 mL of saturated sodium bicarbonate. ... range of structural diversity produced by a sequence of biosynthetic reactions. Cyclization of oxidosqualene is the ini- tial origin of structural diversity...

Ngày tải lên: 19/02/2014, 07:20

... number of genes in U6A and U6A-2 cells, IFN-treated U6A-VV-II cells showed the down-regulation of a substantially larger subset of genes. Identification and characterization of a subset of ISGF3-independent ... & Zdanov A (2003) Characterization of the recombinant extracellular domains of human interleukin-20 receptors and their complexes with inter- leukin-19 an...

Ngày tải lên: 19/02/2014, 07:20

... treated with 2 mm AEBSF before and ⁄ or after SDS ⁄ PAGE and subsequent renaturation of separated proteins. The pretreatment of the sample with the inhibitor par- tially blocked only the 75 kDa activity, ... Minis- try of Education, Culture, Sports, Science and Tech- nology and from the Ministry of Welfare and Labor of Japan. References 1 Jones JI & Clemmons DR (1...

Ngày tải lên: 19/02/2014, 07:20

... tACE, WT Enzyme activity, % of activity at 0.5 M NaCl R169Q R514Q 100 80 60 40 20 0 Fig. 5. Activities of wild-type and R169Q and R514Q ACE2 mutants in the absence (grey) and presence (black) of NaCl ... first investigation of the importance of key active-site resi- dues of ACE2 through site-directed mutagenesis, with the aim of providing practical evidence for their...

Ngày tải lên: 20/02/2014, 01:20