Báo cáo khoa học: Identification of crucial residues for the antibacterial activity of the proline-rich peptide, pyrrhocoricin pot

... inside the surface. Due to the lack of ordered structure C-terminal to Ala606, the rest of the synthetic DnaK peptide is excluded from this graphical representation. The crucial residues for pyrrhocoricin ... ordered conformation. Identification of the pyrrhocoricin binding surface on E. coli DnaK Encouraged by the success of the Ala-scan in identifying the...

Ngày tải lên: 08/03/2014, 10:20

... determine whether RGG3 of EWS modulates the formation or unwinding of Htelo G-quadruplex DNA. To determine whether the RGG3 binding affected the stability of the G-quadruplex structure of Htelo, we performed ... equilibrium G-quadruplex forma- tion of some antiparallel form of the hybrid paral- lel ⁄ antiparallel (3 + 1) form together with the parallel propeller form a...

Ngày tải lên: 15/02/2014, 01:20

... because of the large size of some of the introns, they are not. For stat6, potential missing human exons in zebrafish are indicated by ‘*’. The EMBL accession numbers of the T-bet genes are as follows: ... zffoxp3-R2 for foxp3. These primers amplified part of the initial predicted sequence which contained the majority of the open reading frames of zebrafish stat6,...

Ngày tải lên: 16/02/2014, 09:20

... at 80 °C for 18 h. The FAMEs were recovered into the organic phase by three-fold extraction with 50% NaCl ⁄ chloroform (1 : 1 v ⁄ v). A new volume of chloroform was used for each of the three ... found the lgt5 gene encoding an a-galactosyltransferase for addition of the terminal galactose of the LOS [25], and Schwin- gel et al. found the lgt6 gene involved in the...

Ngày tải lên: 18/02/2014, 14:20

... transduce the signals through the subtegument, thus enhancing the ability of the parasite to respond to changes in the sur- rounding environment. Therefore, the expression pattern for SmSmad1B ... Leu, – Trp) for LacZ filter-lift assays. The development of a blue color through the activation of the yeast LacZ reporter gene is another indication of a protein–protei...

Ngày tải lên: 18/02/2014, 16:20

... of the CA D. melanogaster sequences and, on the other hand, the other D. melanogaster sequences more closely related to the CAVI vertebrate isoforms (CAa, CAb and D. melano- gaster-3) or to the ... amplification of the purified PCR product was performed with the reverse primer RpCAtrR probe only to enrich the PCR product for antisense RpCAtr fragments. Then, addition of...

Ngày tải lên: 18/02/2014, 16:20

... constitutive isoforms of HSP70 are synthesized in the gills and mantle of C. gigas [26]. The expression level of the constitutive forms increases after thermal stress, whereas the inducible one ... synthesized and ⁄ or renatured under these conditions. The upregu- lation of all of these chaperones confirms the severity of the thermal stress under our experimental cond...

Ngày tải lên: 18/02/2014, 16:20

... immunolocalization suggests the potential function of OMM-64. In the inner ear sacculus, the otolith is close to the proximal side of the sacculus, and calcification of the otolith occurs mainly at the proximal ... [20]. Therefore, the proximal region of the sacculus pro- duces the otolith matrix proteins [13] and forms the environment for otolith mineralization...

Ngày tải lên: 18/02/2014, 17:20

... identical to that of the authentic olean-3b,24-di- acetoxy-12-ene, except for the presence of several back- ground peaks (the amount of authentic sample was adjusted to equalize the height of both peaks ... sug- gesting the strict substrate specificity of CYP93E1 for the 3-hydroxyolean-12-ene structure. The 24-hydroxylase activity of CYP93E1 for b-amy- rin and...

Ngày tải lên: 19/02/2014, 07:20

... 1573 expression profile of cells expressing the VV-II mutant form of STAT2 was more similar to that of U6A cells than that of U6A-2 cells (Level 1). IFN treatment led to the further segregation of the sample ... analysis The analysis of the microarray results presented in Table 1 required the use of an arbitrary ‘cut-off’ value for level of gene expression, an app...

Ngày tải lên: 19/02/2014, 07:20