Báo cáo khoa học: Structure and function of N-acetylglucosamine kinase Identification of two active site cysteines pptx

... FEBS MINIREVIEW Structure and function of active chromatin and DNase I hypersensitive sites Peter N. Cockerill Experimental Haematology, Leeds Institute of Molecular Medicine, University of Leeds, ... Involvement of histone H1 in the organization of the nucleosome and of the salt-dependent superstructures of chromatin. J Cell Biol 83, 403–427. Active chromatin and...

Ngày tải lên: 14/02/2014, 18:20

... The anchorage of the prosegment and of part of the N-terminus in the active site cleft is made by ionic interactions established between Lys11/Tyr13 of the mature enzyme sequence and the catalytic ... (1990) The structure and function of the aspartic proteinases. Annu. Rev. Biophys. Biophys. Chem. 19, 189–215. 2. Dunn, B.M. (2002) Structure and mechanism of the p...

Ngày tải lên: 19/02/2014, 12:20

... which the b1-strand and b2-strand are adjacent and parallel to each other, and the b¢-strand is adja- cent and antiparallel to the b1-strand (Fig. 1). The length and sequence of the variable ... a1-helix and a2- helix and the b2-strand of KH1. Intimate contacts between protein and RNA continue across the cusp of the KH1 and KH2 domains. C46 binds to the Fig. 6. Crystal...

Ngày tải lên: 07/03/2014, 05:20

... phosphorylation by N-acetylglucosamine kinase. In this study we describe the identification of two active site cysteines of the sugar kinase by site- directed mutagenesis and computer-based structure prediction. ... comparison of GlcNAc kinase with a model of the three-dimensional structure of glucokinase provides the first picture of the possible structure...

Ngày tải lên: 08/03/2014, 10:20

... form and function of novel complexes such as HAMLET and ELOA, we have provided new evidence that a loss of native structure can endow proteins and their complexes with distinct and beneficial functions substantially ... ago and since then the HAMLET field has widened in scope, acquiring new members and enriching our understanding of the basic principles underlying protein...

Ngày tải lên: 29/03/2014, 21:20

... including Trp227 A )is13A ˚ from the DAB active site (measured from the Ca of Arg312; Fig. 4), 42 A ˚ from active site ABC, 36 A ˚ from active site BCD, and 42 A ˚ from act- ive site CDA. It is therefore possible ... (A) Comparison of the active sites. The A. radiobacter S2 CMLE1 (ArCMLE1) active site is in gray, and the P. putida CMLE (PpCMLE) active site is i...

Ngày tải lên: 30/03/2014, 10:20

... extent of membrane localization of Noxo1b and Noxo1c. The intensities of immunoreactive bands for HA-Noxo1b and HA-Noxo1c in (B) were quantified using a LAS-1000plus (Fuji film) image analyzer and ... Functional Analyses from the Ministry of Education, Culture, Sports, Science and Technology of Japan, and CREST and BIRD projects of JST (Japan Science and Technology Age...

Ngày tải lên: 19/02/2014, 06:20

... self-assembly of the envelope glycoprotein of ASLV bound to membrane. Effects of pH on the secondary structure and membrane interaction of IFP–wt Based o n studies on the molecular clustering of EnvA ... Kantchev and Ding-Kwo Chang Institute of Chemistry, Academia Sinica, Taipei, Taiwan, Republic of China The structure and membrane interaction of the internal fusion...

Ngày tải lên: 19/02/2014, 16:20

... maturation and export of the two ribosomal subunits [1]. Most of the constitu- ents of the preribosomal particles have been identified in yeast by exploiting the potent combination of genetic and biochemical ... of RP mRNA for adjustment of the biosynthesis of ribosomes to the requirements of cell growth and differentiation. In addition, a relevant contribution of pro...

Ngày tải lên: 07/03/2014, 01:20

... in binding of the cofactor ThDP are located at the C-terminal ends of the b-strands of Dom-c (diphosphates and Mg 2+ ) and of Dom-a¢ of a neighbouring subunit (pyrimidine moiety). The active centre ... pathways and catalyse a broad range of reactions mainly involving the cleavage and the formation of C–C-bonds. For instance, they catalyse nonoxidative and oxidative d...

Ngày tải lên: 07/03/2014, 12:20