Báo cáo khoa học: Functionally active fusion protein of the novel composite cytokine CLC/soluble CNTF receptor pdf

... m. Gene. Ther. 11, 1177–1187. Ó FEBS 2002 Bioactive CLC/sCNTFR fusion protein (Eur. J. Biochem. 269) 1941 Functionally active fusion protein of the novel composite cytokine CLC/soluble CNTF receptor Catherine ... Binding of the cytokine to the membrane-bound, nonsignaling a chain (CNTFR; [21]), l eads to the recruitment of t he sha red signaling subunit...

Ngày tải lên: 08/03/2014, 10:20

... respectively, of that of HPRT. This low activity could be explained by the fact that PRTFDC1 has a Gly in the position of the proposed catalytic Asp of HPRT. In PRTFDC1, a water molecule at the position of ... and regulators of other enzymes in the human nucleotide metabolism (Table S1). The library can then be seen to constitute the specific metabolome of the nuc...

Ngày tải lên: 15/02/2014, 01:20

... stabilization of active RhoA by OTUB1 prior to bacterial invasion. The accumulated pool of active RhoA contributes to an enhanced uptake in the early phase of invasion, con- sistent with the involvement of ... Kessler Department of Clinical Medicine, University of Oxford, UK Introduction The genus Yersinia consists of three pathogenic species that are agents of a v...

Ngày tải lên: 16/02/2014, 15:20

... centrifugation of the samples, proteins bound to liposomes were analyzed by SDS ⁄ PAGE of the resulting pellets. (C) The amounts of I C in the pellets. The amounts of I C were quantitated with the UN-SCAN-IT ... phospholipid and membrane binding of the other members of the PEBP family. Consequently, the binding characteristics of PEBP proteins, including those...

Ngày tải lên: 19/02/2014, 05:20

... that the fusion peptides of gp41 of HIV-1 and HA2 do not span both leaflets of the membrane bilayer [ 15,22]. The I FP of ASLV-A is shown to penetrate more shallowly into the membrane than the N-terminal ... in analogy to the IFP of class II fusion proteins [10,11]. In conjunction with the model proposed for the class II fusion proteins, it is c oncluded t hat a...

Ngày tải lên: 19/02/2014, 16:20

... proliferation of LXF-289 lung tumor cells. Effects of P2 receptor agonists. The influence of the different P2 receptor agonists is shown as percentage inhibition of the prolifer- ation of LXF-289 ... per- centages of the cells in the G0 ⁄ G1, S and G2 ⁄ M phases of the cell cycle after treatment with 20 l M or 100 lM ATP for 24 h. The gat- ing used for the qua...

Ngày tải lên: 07/03/2014, 12:20

... and by increasing the pK a of the catalytic acid. The hydroxyl group of Tyr214 makes a major contribution to the positioning of the N-acetyl group of the )1sugar.Taken together, the results show ... 257 rows 5 and 7 shows that in the presence of the substrate, rotation of Asp142 towards Glu144 lowers the pK a of the latter by 0.8 pH units. The calculated...

Ngày tải lên: 07/03/2014, 14:20

... these residues stabilize the distortion of the NAG3 residue. In these interac- tions, we particularly focused on the Trp664 residue, which is located at the bottom of the active site cleft. The ... result of con- formational changes, but of the removal of negative charge at these residues. Conformation of chito-oligosaccharides bound to the active site cleft Fo...

Ngày tải lên: 15/03/2014, 11:20

... analyzed the influence of CHIP on the ATPase cycles of both chaperones in the presence of co-chaperones and a substrate, and determined the ubiquitination efficacy of CHIP in the presence of the chaperones. ... influences the chaperone cycle of Hsp70 proteins to prolong the life-time of the substrate–Hsp70–CHIP complex and thereby to increase the possibility of...

Ngày tải lên: 15/03/2014, 23:20

... in the brain and further displays a number of structural features not present in the other members of the APP superfamily, such as the absence of the exon encoding the KPI domain and the lack of ... [3,4,13,26]. Comparative analysis of the Xenopus and mammalian APLP2 proteins Comparing the amino acid sequences of the two X-APLP2 proteins with the human, mouse...

Ngày tải lên: 16/03/2014, 16:20