Báo cáo khoa học: Biosynthesis of riboflavin 6,7-Dimethyl-8-ribityllumazine synthase of Schizosaccharomyces pombe pot

... et al. Biosynthesis of riboflavin – generation of an improved cyclohydrolase II FEBS Journal 276 (2009) 4119–4129 ª 2009 The Authors Journal compilation ª 2009 FEBS 4129 Biosynthesis of riboflavin Screening ... 3,4-Dihydroxy-2-butanone 4-phosphate synthase. (D) 6,7-Dimethyl-8-ribityllumazin synthase. (E) Riboflavin synthase. Biosynthesis of riboflavin – generation of an...

Ngày tải lên: 18/02/2014, 11:20

... Biosynthesis of riboflavin 6,7-Dimethyl-8-ribityllumazine synthase of Schizosaccharomyces pombe Markus Fischer 1 , Ilka Haase 1 , Richard Feicht 1 , ... reactions in the pathway of ri boflavin biosynthesis. (A) Lumazine synthase; (B ) riboflavin synthase. 520 M. Fischer et al. (Eur. J. Biochem. 269) Ó FEBS 2002 Transformation of E. coli XL1-Blue cells E. ... formate. R...

Ngày tải lên: 08/03/2014, 10:20

... acceleration of that reaction by lumazine synthase from Fig. 6. Boundary sedimentation. A mixture of 0.5 mg of each of the lumazine synthase from B. subtilis and M. jannaschii (per mL of 50 m M potassium ... Schizosaccharomyces pombe, Ó FEBS 2003 Biosynthesis of riboflavin (Eur. J. Biochem. 270) 1027 are in the range of 200–250 nmolÆmg )1 Æmin )1 (Table 3). The cataly...

Ngày tải lên: 23/03/2014, 21:20

... identifi- cation of labile 4-keto UDP-sugars [32]. Monitoring of the anomeric region of the 1D- 1 H-spectrum over the course of incubation with His6-GMD, without NADPH, showed progressive depletion of signals ... growth of peaks corresponding to the anomeric resonances of the 4-keto (compound B) and gem-diol (compound C) forms of GDP-6-deoxy-d-lyxo-hexos-4-ulose (Fig. 4B). On t...

Ngày tải lên: 16/03/2014, 01:20

... absence of any detect- able excess of the 13 C 13 C coupling satellites beyond the natural abundance level for the signals of C-3 and C-4 of a hypothetical product 1c. The hypothetical posi- tions of ... (based on the concentration of 1) during a period of 24 h at 37 °C, irrespective of the presence or absence of IspC. Using an equilibrium mixture of [U- 13 C 5 ]-1 and of...

Ngày tải lên: 16/03/2014, 06:20

... pathway of i soprenoid biosynthesis, the conversion of 2 C-methyl- D -erythritol 4-phosphate into its cyclic diphosphate proceeds via nucleotidyl intermediates and is catalyzed by the products of ... however, the similarity of the hypothetical C. jejuni gene to ispF is higher t han the similarity to ispD. Many residues previously shown to be part of the catalytic sites of the Is...

Ngày tải lên: 23/03/2014, 13:20

... heterologous cells. A pproaches of this type have improved our understanding of the requirements for normal biosynthesis of the GPIb–IX complex and of the consequences of mutations encountered in ... trafficking of GPIb a. These results indicate that correct biosynthesis and surface expression of GPV in platelets requires the presence o f the other s ubunits of the G PIb–V–I...

Ngày tải lên: 23/03/2014, 13:20

... Richter G (2000) Biosynthesis of vitamin b2 (riboflavin) . Annu Rev Nutr 20, 153–167. 2 Fischer M & Bacher A (2008) Biosynthesis of vita- min B2: structure and mechanism of riboflavin syn- thase. ... important set of biochemical reactions. Other cofactors, on the other hand, perform very different chemical tasks, and compete for the title of master of versatility, with vit...

Ngày tải lên: 28/03/2014, 22:20

... Richter G (2000) Biosynthesis of vitamin b2 (riboflavin) . Annu Rev Nutr 20, 153–167. 2 Fischer M & Bacher A (2008) Biosynthesis of vita- min B2: structure and mechanism of riboflavin syn- thase. ... important set of biochemical reactions. Other cofactors, on the other hand, perform very different chemical tasks, and compete for the title of master of versatility, with vit...

Ngày tải lên: 28/03/2014, 22:21

... scheme of biosynthesis of D-arabinofuranosyl residues of the cell-wall arabinogalactan and lipoarabinomannan in mycobacteria, including a feedback mechanism and possible sites of action of ethambutol, ... almost 15 years of intensive research all over the world, and our better knowledge of the structure, functions and biosynthesis of the mycobacterial cell wall, the mode...

Ngày tải lên: 30/03/2014, 04:20