ANTIBIOTIC RESISTANCE OF ESCHERICHIA COLI ISOLATED FORM POULTRY WORKERS, PATIENTS AND CHICKEN IN THE EASTERN PROVINCE OF SAUDI ARABIA ppt
... investigate and compare the patterns of resistance of E. coli isolated from poultry industry workers, patients and chicken, and to identify the common serotypes of E. coli isolated from humans and poultry. Materials ... demonstrate alarmingly high individual and multiple resistance to antibiotics in E. coli, reflecting the misuse of these agents...
Ngày tải lên: 08/03/2014, 09:20
... the sequences of 1CEV and 1RLA and pasted into the structural alignment. The alignment was then corrected by hand. Since the sequences of the two arginases and agmatinase greately differ in the ... agmatinase with the GfiAandCfiT substi- tutions in the coding and noncoding strands, respectively, and using the 5¢ and 3¢ primers mentioned above, the full le...
Ngày tải lên: 21/02/2014, 01:21
... prerequisite for the accessibility of the inner core sugars for the binding bymAbWN1222-5. Previously, an NOE between the anomeric proton of the side-chain heptose (residue H) and the axial H-3 of the first ... (strong) and H-3e (very weak) of a-Kdop (residue C) and H-1 of L , D -Hepp (residue H) and between the anomeric proton of the inner L , D -Hepp res...
Ngày tải lên: 08/03/2014, 09:20
Tài liệu Báo cáo khoa học: Role of K22 and R120 in the covalent binding of the antibiotic fosfomycin and the substrate-induced conformational change in UDP-N-acetylglucosamine enol pyruvyl transferase docx
... of R120 by lysine reduces the probability of forming the fully closed form of the protein. The results obtained with the K22V/R120K double mutant protein point toward a central role of arginine ... two other positively charged amino acid side chains, R397 and R120, are also involved in fosfomycin binding, and in view of the multitude of interactions, it was a...
Ngày tải lên: 19/02/2014, 13:20
Báo cáo khoa học: Role of DptE and DptF in the lipidation reaction of daptomycin ppt
... is GLDSV, indicating that this putative ACP domain is one of the few ACPs in which valine replaces isoleucine (I) or leucine (L) in the conserved sequence. To determine whether DptF is the putative ... of DptE, we expressed dptF using the pQTev vector in E. coli and purified the resulting ACP as an N-terminal His7 fusion protein (Fig. 3) with a yield of 9.5 mgÆL )1 o...
Ngày tải lên: 07/03/2014, 04:20
Báo cáo khoa học: Investigating the role of the invariant carboxylate residues E552 and E1197 in the catalytic activity of Abcb1a (mouse Mdr3) docx
... of the trypsin-sensitive region situated in the linker domain of Abcb1a. Fol- lowing trapping in the absence or presence of Vi and mild-trypsin treatment, the trypsin degradation pro- ducts of ... trapping is almost normal. By contrast, when the charge is removed, as in the glutamine (length of the side chain maintained) and asparagine (shorter side chain) mu...
Ngày tải lên: 07/03/2014, 06:20
Báo cáo khoa học: "Some Comments on Algorithm and Grammar in the Automatic Parsing of Natural Languages" ppt
... written in the form of appropriate tables of rules. The paper raises the question of the increasing complexity of the tables when more than the most elementary natural-language con- ditions are included, ... Examples of this are very common in English and include many of the frequently cited instances of nesting. The second consideration is that the...
Ngày tải lên: 07/03/2014, 18:20
Tài liệu Báo cáo khoa học: Ionic strength and magnesium affect the specificity of Escherichia coli and human 8-oxoguanine-DNA glycosylases pdf
... higher than for the THF:A ligand. The presence of Mg 2+ had little effect on the binding of Fpg to the THF:C ligand and slightly improved binding to the THF:A ligand, making it comparable with binding to ... decrease in binding at 0 and 500 mm KCl [32]. Thus, to address the in uence of the reaction conditions on the binding of Fpg and OGG1 to damaged DNA, we d...
Ngày tải lên: 18/02/2014, 18:20
Tài liệu Báo cáo khóa học: The C-terminal domain of Escherichia coli Hfq increases the stability of the hexamer ppt
... between the N-terminal domain of Hfq and the Sm and Sm-like (L-Sm) proteins of eukaryotes and archaea [11,15,16]. These proteins are components of the spliceosome complex and are also involved in other ... structure. The absence of this domain results in a reduction of the b-sheet character within the Sm domain and could perturb the hydrogen bonding inter...
Ngày tải lên: 19/02/2014, 12:20
Tài liệu Báo cáo khoa học: Photochemical cross-linking of Escherichia coli Fpg protein to DNA duplexes containing pdf
... retardation band, which indicates complex formation between DNA duplex II and the Fpg protein. The intensity of the retardation band increased with increasing Fpg protein concentration. The binding reaction ... duplexes E. coli Fpg protein recognizes a hexanucleotide sequence with oxoG in the middle in the lesion-bearing DNA strand and specifically binds to it and th...
Ngày tải lên: 20/02/2014, 11:20