Báo cáo khoa học: Pherokine-2 and -3 Two Drosophila molecules related to pheromone/odor-binding proteins induced by viral and bacterial infections ppt

... (Eur. J. Biochem. 270) 3407 Pherokine-2 and -3 Two Drosophila molecules related to pheromone/odor-binding proteins induced by viral and bacterial infections Laurence Sabatier 1, *, Emmanuelle Jouanguy 1, *†, ... was analyzed by HPLC and the molecule induced by the viral infection was detected by MALDI-TOF MS (Fig. 2B). The molecule was purified to...

Ngày tải lên: 08/03/2014, 08:20

... a nd Der p 1 in Pichia pastoris was recently r eported by Yasuhara et al.[23]andBestet al. [24,25], respectively. Maturation of rpro-Der f 1 was shown to be induced by dialysis against pH 4.0, ... ature and pro-Der p 1 were obtained by PCR (Table 1). Subse- quently, they were cloned into pPICZaA(andpGAPZaA) in-frame with the s ecretion peptide (Table 1). DNA sequences were d eterm...

Ngày tải lên: 17/03/2014, 11:20

... 4·-RNase HI and 5·-RNase HI proteins by 27.0, 14.8 and 7.0 kJÆmol )1 and 3.1, 1.7 and 0.8 m, respectively. Thus, the stabilities of the 5·-RNase HI and 6·-RNase HI proteins against urea- induced ... residues 29, T34 and E131 are shown. The hydrogen bonds between N29 and Oc and T34 and Oc, and between N29 and Nd and E131 and Oe2, in the wild-type protein are s...

Ngày tải lên: 18/02/2014, 13:20

... 5¢-GAGAATCCAGCTTCTTTCCC-3¢ and antisense, 5¢-GGCGACACTGTATGAATTGC-3¢); SENP2 (sense, 5¢-AACAGTCTCTACAATGCGGCCA-3¢ and antisense, 5¢-CCGTGTTCCATTACAAGCAGAA-3¢); SAE1 (sense, 5¢-GACCTGCTTCCCGATGACTTT-3¢ and antisense, ... 5¢-TTCCTCCAACCACAGCACATAC-3¢); UBC9 (sense, 5¢-CAACAAAGAACCCTGATGGCACGA-3¢ and antisense, 5¢-GCATCCGTAGCTTGAACAAGCCTC-3¢); PIAS3 (sense, 5¢-ACTGCAGGGACCCTGCTACA-3¢ an...

Ngày tải lên: 23/03/2014, 06:20

... conserved sequence signatures to early deuterosto- mes [12,13,21–23], and indeed to lophotrochozoans, prebilaterans and even unicellular eukaryotes, although it appears to have been lost throughout ... mediterranea, and the sea anemone Nematostella vectensis. However, human pFGE and also pFGE from 20 further mammalian species (from various primates to squirrel, bat, dolphin, s...

Ngày tải lên: 23/03/2014, 07:20

... dodecyl sulfate to a dilute aqueous solution, Ab(1–40) is converted to b-sheet, as observed by CD at 3 °C and 20 °C. The transition is mainly a two- state process, as revealed by approximately ... structured state and conversion to b-sheet and then to a-helix are evident. The CD intensities at this wavelength allowed us to compare the detergent secondary structure inducti...

Ngày tải lên: 30/03/2014, 02:20

... (A) and FVIIa (B) with sTF. The curves represent analyte injected at 20, 40, 80, 160 and 320 n M, respectively, from bottom to top. Hydrogen bonds involving Gly372 in factor VIIa E. Persson and ... TF-interactive helix and the 170 loop (red), and the covalently attached inhibitor fFR-cmk (purple). The hydro- gen bonds from Gly372(223) to Arg315(170C) and Ser-333(185) are indic...

Ngày tải lên: 18/02/2014, 08:20

... [30,31]. Accumulation of mitoDC-81 by isolated mitochondria An ion-selective electrode sensitive to mitoDC-81 was constructed in order to determine whether mitoDC-81 was accumulated by energized mitochondria ... electrode to cation concentra- Fig. 2. Uptake of mitoDC-81 by energized mitochondria. An ion- selective electrode was used to measure the uptake of mitoDC-81 into rat li...

Ngày tải lên: 20/02/2014, 11:20

... cold- and ischemia -induced apopto- sis in donor livers and reduces sinusoidal endothelial cell apoptosis and caspase-3 activity by 94%. It has recently been granted orphan drug status for liver and ... caspase-3 inhibitors can block PC12 apoptosis induced by Ab(25–35) and that they are not toxic for PC12 cells. The effect of compound 1 on cytotoxicity induced by Ab was a...

Ngày tải lên: 07/03/2014, 11:20

... against toxic xenobiotics and byproducts of oxidative metabolism. In addition to their catalytic activities, plant GSTs are also involved in the response to different biotic and abiotic stresses, and ... A ˚ 2 ,andthe B-factors of S atoms are 38.14 A ˚ 2 and 79.30 A ˚ 2 , respect- ively. It is therefore reasonable to propose that conform- ational changes and changes in dynamic...

Ngày tải lên: 16/03/2014, 16:20