Báo cáo khoa học: Uptake and metabolism of [3H]anandamide by rabbit platelets Lack of transporter? ppt

... Uptake and metabolism of [ 3 H]anandamide by rabbit platelets Lack of transporter? Lambrini Fasia, Vivi Karava and Athanassia Siafaka-Kapadai Department of Chemistry (Biochemistry), ... involvement of FAAH on [ 3 H]anandamide uptake and metabolism by rabbit platelets, the effect of phenylmethylsulfonyl fluoride on [ 3 H]ananda- mide uptake was determin...

Ngày tải lên: 08/03/2014, 08:20

... equation of Fluo-3 for Ca 2+ [27]. Ultra-pure calcium-free water was used for preparation of all buffers and (ant)agonists. Measurement of cytosolic cAMP and InsP 3 Intracellular levels of cAMP and ... Ca 2+ response, and abolished the effects of ADP, AR-C and wortmannin AB CD Fig. 6. Contribution of SERCA and phospholipase C to P2Y 12 -dependent prolongation of Ca 2...

Ngày tải lên: 18/02/2014, 16:20

... model of language ability, and therefore is often used as an index of language proficiency and development status of L2 learners. Various studies have proposed and inves- tigated measures of ... relevant to be evaluated both by a human rater and an automated scoring system. Examples of such aspects of speech, which are considered part of the construct 1 of “c...

Ngày tải lên: 07/03/2014, 22:20

... boundary determination by balanc- ing a comprehensive set of sentence com- plexity and quality related constraints. Second, our approach can simulate the characteristics and the style of naturally occurring ... syntactic and lex- ical realizability, and its smoothness between sen- tence transitions. Sentences that are too complex are hard to understand, so are sentences lacking...

Ngày tải lên: 20/02/2014, 15:20

... hydroxyl groups are present at positions 20 and 23. Three minor products of D3 metabolism by P450scc were identified by MS and by examining their sub- sequent metabolism by P450scc. These products were 23-hydroxyvitamin D3, ... the major and minor pathways for the metabolism of vitamin D3 by P450scc. Results Metabolism of vitamin D3 by P450scc Six different prod...

Ngày tải lên: 18/02/2014, 17:20

... disulfides, and assayed as above. Thiols were quanti- fied by comparison of peak areas to standard curves of those standards. Coenzyme A was measured as the sum of the areas of the CoA and dephospho ... and thermoactivity of the CoADR phCoADR is stable for months at both )80 °C and )20 °C, and has half-lives of > 100 and 39 h at 85° and 95 °C, respectively. Figure...

Ngày tải lên: 07/03/2014, 17:20

... with binding of both MOF and Tip60 (equivalent to Esa1 in yeast NuA4) [124,130]. However, in human cells depletion of MOF, but not Tip60, results in reduced global levels of AcH4-K16 and defective ... acetylation and deacetyla- tion, and transient recruitment of remodellers and transcription factors. A cycle of transcription commences with the recruit- ment of transcript...

Ngày tải lên: 14/02/2014, 18:20

... expression of estrogen receptor-a (ERa) is autoregulated by miR-18a, -19b and -20b, which in turn are upregulated by the activation of ERa. This mechanism of regulation provides a wide range of coordinated ... Scientific Fund of the Ministry of Education, Science and Culture of Japan (G.T.); the Program for Promotion of Fundamental Studies in Health Sciences of the Na...

Ngày tải lên: 14/02/2014, 19:20

... Department of Biology and Biochemistry, University of Bath, UK 2 Department of Biochemistry, University of Wisconsin–Madison, USA 3 Department of Chemistry, University of Wisconsin–Madison, USA Introduction Upon ... phosphoryl group. Results and Discussion Sulfonimides as inhibitors of RNase A We began by determining the ability of three backbone analogs of RNA to inhi...

Ngày tải lên: 14/02/2014, 22:20

... Model of the homotetrameric quaternary structure of cN-II showing interfaces A and B and the Mg 2+ site. The inset shows the tertiary structure of each subunit. Effector sites 1 and 2 and the ... alteration of this ratio for a mutant was considered as caused either by an alteration of the K m value for one of the two substrates or by a variation of the k cat value fo...

Ngày tải lên: 15/02/2014, 01:20