Báo cáo khoa học: Functional symmetry in the isolated domain demonstrated by N-ethylmaleimide labelling pdf

... Biochem. 270) Ó FEBS 2003 The nucleotide-binding domains of P-glycoprotein Functional symmetry in the isolated domain demonstrated by N -ethylmaleimide labelling Georgina Berridge 1 , Jennifer ... chain to the nucleotide-binding pocket [3]. We describe the engineering, expression and purification of single-cysteine-containing proteins comprising either the C-terminal...

Ngày tải lên: 08/03/2014, 08:20

... two elements in ClpA, one in the N -domain and the other in the pore of the ClpA hexamer. In the case of short unstructured peptides or unfolded proteins such as casein, binding to the tyrosine residues in ... for various adaptor proteins [6–10]. ClpA consists of three domains: an N -domain and two ATP-binding domains referred to as the D1 and D2 domains. Interesting...

Ngày tải lên: 07/03/2014, 05:20

... DEC1 (including the bHLH domain) is sufficient for the suppressive activity of DEC1. The C-terminal region (amino acids 140–412), including the Orange domain [18], i s not required for suppression in ... 4415 whereas the N-terminal region, containing the bHLH domain, is not essential for the interaction. Dominant-negative DEC1 counteracts the suppression of CLOCK/BMAL1-in...

Ngày tải lên: 19/02/2014, 16:20

... removal of the N-terminal helices 1 and 2 while leaving the interacting a-helix 8 intact, may destabilize the proteins leading to spurious protein–protein or/and protein–DNA interactions. The crystal ... migration. The proposed DNA binding role of the triple-helix domain of Ydc2 is further supported by the observation in the NCBI database that the N-terminal region of...

Ngày tải lên: 20/02/2014, 11:20

... is shown in Fig. 9, where the structure around the MPT site in bovine XDH is shown and the corresponding amino acids in C. acidovorans are shown in parenthesis. The residues around the Mo site ... centers and an FAD into the recombinant enzyme. Therefore, the host organism has to be able to provide these cofactors and the means for their incorporation into the expres...

Ngày tải lên: 16/03/2014, 23:20

... from quinoline [1,2]. Besides quino- line, some quinoline derivatives and the benzodiazines quinazoline and quinoxaline are accepted as substrates [1,3]. Like other enzymes catalysing the hydroxylation ... diminuta 7, coding for isoquinoline 1-oxidoreductase, were cloned in P. putida KT2440 and in the quinoline degrading strain, P. putida 86. However, the level of Ior synthesis wa...

Ngày tải lên: 17/03/2014, 10:20

... fractions containing P-gp, the results could be affected by other proteins. In fact, it was reported that the linker region of P-gp interacts with other proteins such as RING finger protein 2 (RNF2) ... participate in neither the preservation of the overall structure nor the ATPase reaction itself. This is supported by the following findings. (i) Cleaving the linker did no...

Ngày tải lên: 29/03/2014, 23:20

... recovered, only the longest amino acid sequences that included the DNA binding, hinge and ligand-binding domains were used for this analysis, except for the Knirps fam- ily members that lack the LBD. In D. ... vitellogenic female mosquitoes. They isolated several clones that code for a single protein. Interestingly, during the initial process of identifying the NR family membe...

Ngày tải lên: 18/02/2014, 13:20

... interpretation in distinguish- ing interacting from noninteracting proteins. One Fig. 1. Schematics of library designs. The helical wheel diagram looks down the axis from the N-terminus to the C-terminus. ... importance in our abil- ity to engineer increased protein–protein interaction stability at will; in particular, the ability to increase stability by kinetic design. For...

Ngày tải lên: 18/02/2014, 13:20

... 2; RIG-I, retinoic acid-inducible gene I; siRNA, small interfering RNA; TBK, Tank-binding kinase 1; TLR, Toll-like receptor; TRIF, Toll-IL1 receptor domain- containing adaptor inducing IFN-b. FEBS ... [8–10]. Virus-infected cells trigger the innate immune response by recognizing viral components, including DNA, ssRNA, dsRNA and glycoproteins. This response initiates signaling pathways le...

Ngày tải lên: 18/02/2014, 16:20