Báo cáo khoa học: Transcription termination at the mouse mitochondrial H-strand promoter distal site requires an A/T rich sequence motif and sequence specific DNA binding proteins pptx

... termination at the mouse mitochondrial H-strand promoter distal site requires an A/T rich sequence motif and sequence specific DNA binding proteins Vijayasarathy Camasamudram, Ji-Kang Fang and Narayan ... L and H strand promoters (LSP and HSP), L and H strand transcription start sites (LSS and HSS). The L -strand sequence and position...

Ngày tải lên: 08/03/2014, 08:20

... indicates that, for all treatments, the FI rise was faster at 25 °C than at 15 °C and that the contribution from the O–J phase decreased at high temperature. Figure 3 repre- sents the amplitudes and ... in the FI curves depends on the relative amplitude and rate constant of the J–I phase compared to the amplitudes and rate constants of the two other phases. Th...

Ngày tải lên: 19/02/2014, 05:20

... mutants, the relationship between the DEC1–BMAL1 interactio n and transcriptional suppression by DEC1. We also evaluated the E-box -binding activity of these mutants. Our results showed that the ... blot analys is with anti-FLAG immunoglobulin (left-hand panel). The transcriptional activities of FLAG-BMAL1 and FLAG-DEC1 were confirmed by using the luciferase assay (right-hand...

Ngày tải lên: 19/02/2014, 16:20

... Tec-920 and Tec-1800; two putative NF-jB binding sites are indicated, and the known PU.1 and SP1 binding sites are also shown. (B) Schematic representation showing the potential NF-jB binding sites ... to the Tec promoter and induces Tec transcription. (A) The chromatin of A20 cells was cross-linked, sheared and immunoprecipitated with the indicated antibodies. In...

Ngày tải lên: 07/03/2014, 00:20

... the negative control fraction remained in a final fraction. There were also two bands at 65 kDa and 72 kDa that were detected in both the negative control and final fractions. The p56 and p68 proteins ... [18–20] and within the 5¢-UTR of the human very low density lipoprotein receptor gene (VLDLR) [21]. Such GCC- elements are transcriptionally active and can interact spe...

Ngày tải lên: 07/03/2014, 05:20

... of the mouse and human R2 DNA sequences around their transcription start sites. Capital letters in bold style indicate nucleotides that are conserved between human and mouse; arrows indicate the ... numbers represent the intensity of each band normalized to the band in lane 1. The lower numbers represent the intensity of the bands in lanes 5 and 6 normalized to the...

Ngày tải lên: 08/03/2014, 02:20

... concur that there is no meaningful chance of reanimation [8]. Some reasons why this occurs are as follows: 1. Physicians tell surrogates that they can make any decision they want as an open-ended ... regarding treatment options because of their proximate knowledge of what the patient would have wanted before they became incompetent [7]. This position is based on the postulate that any a...

Ngày tải lên: 25/10/2012, 10:45

... ion and the addi- tional water oxygen were only 0.03 and 0.04 A ˚ , respec- tively, out of the equatorial plane defined by the other three atoms (Table 3). The angles around the metal ion indicated ... Sedimentation equilibrium distribution of ApeSOD. The line reflects the best fit of the data and indicates that the apparent molecular mass is 96 265 Da. The deviation be...

Ngày tải lên: 14/02/2014, 22:20

... PAGE and R2F-antibody as a marker to assist in the purification of the R2F-protein. In the new puri- fication strategy that was developed (see Materials and methods), an increase in the putative ... man- ganese in the transcriptional regulation of the nrd operon [35] may not be considered in the light of the results obtained in the present study. Rather, the involvemen...

Ngày tải lên: 15/02/2014, 01:20

... condensation and DNA fragmentation [1,2], apoptosis can cause irreversible loss of terminally differentiated cardiac myocytes and, therefore, contributes significantly to the pathogenesis of many ... in the nucleolus, nucleus and cytoplasm of the cell, as well as at the cell surface [10]. Nucleolin ⁄ C23 regulates many aspects of DNA and RNA metabolism, such as RNA polyme...

Ngày tải lên: 16/02/2014, 09:20