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Báo cáo khoa học: Distinguishing between different pathways of bilayer disruption by the related antimicrobial peptides cecropin B, B1 and B3 pptx

Tài liệu Báo cáo khoa học: Interactions between M proteins of Streptococcus pyogenes and glycosaminoglycans promote bacterial adhesion to host cells pdf

Tài liệu Báo cáo khoa học: Interactions between M proteins of Streptococcus pyogenes and glycosaminoglycans promote bacterial adhesion to host cells pdf

... involved in the interaction as the binding of 125I-labelled DS was reduced to isogenicderivatives of the AP1 strain lacking either of these surfaceproteins (Fig. 2). The significance of the M1 protein ... shown). Takentogether, these results suggest that the interaction between S. pyogenes and GAGs is mediated by M protein.Mapping of the DS binding region in proteins M1 and M5To define the region responsible ... fibrinogento the NH2-terminal half (A B3) , for IgG to the central Sdomain, and for human serum albumin to the C-repeats(C1–C3) [31]. None of these protein ligands was able toinhibit the binding of 125I-labelled...
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Tài liệu Báo cáo khoa học: Interaction between very-KIND Ras guanine exchange factor and microtubule-associated protein 2, and its role in dendrite growth – structure and function of the second kinase noncatalytic C-lobe domain docx

Tài liệu Báo cáo khoa học: Interaction between very-KIND Ras guanine exchange factor and microtubule-associated protein 2, and its role in dendrite growth – structure and function of the second kinase noncatalytic C-lobe domain docx

... gifts of the MAP2 cDNA and the pCAG expression vector,respectively. The present study was supported by grants from the Japanese Ministry of Education,Culture, Sports, Science and Technology; the ... 1657Discussion The present study revealed the structural and func-tional determinants of the protein–protein interaction between v-KIND and MAP2 (i.e. the MAP2-bindingcore module in v-KIND and the v-KIND-binding ... and 482) and one Thr residue (amino acid 487) were well conservedin the KIND2 of v-KIND in all species analyzed,although not in the other KINDs. To investigate the possible involvement of these...
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Tài liệu Báo cáo khoa học: Competition between innate multidrug resistance and intracellular binding of rhodamine dyes pdf

Tài liệu Báo cáo khoa học: Competition between innate multidrug resistance and intracellular binding of rhodamine dyes pdf

... comparison of rhodamine uptake, in the presence and the absence of uncouplers, allows the estimation of the effect of intracellular binding on the MDR phenomenon. The data detailed obtained in the present ... the cell linestested and the efflux of rhodamines in MRP1 over-expressing cells wasabolished by NBD-Cl, it appears that rhodamines are excluded from thesecells by MRP1. On the other hand, the ... with the accumulation of dyes, and probably certain drugs,in the cytoplasm. On the other hand, high-affinitybinding of these agents to intracellular receptors over-whelms the low levels of ABC...
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Tài liệu Báo cáo khoa học: Autophagy inhibits reactive oxygen species-mediated apoptosis via activating p38-nuclear factor-kappa B survival pathways in oridonin-treated murine fibrosarcoma L929 cells doc

Tài liệu Báo cáo khoa học: Autophagy inhibits reactive oxygen species-mediated apoptosis via activating p38-nuclear factor-kappa B survival pathways in oridonin-treated murine fibrosarcoma L929 cells doc

... proliferation and cell death [27]. The functionalroles of the activation of these kinases are often con-troversially discussed. The ERK1 ⁄ 2 cascade isregarded to be the main method of transmission ... Because many ther-apeutic agents eliminate tumor cells by inducing apop-totic cell death, the further understanding of the apoptotic mechanisms is required for the prevention and treatment of many ... translocation and cytochrome c release are suppressed by NACTo investigate the effects of ROS on Bax translocation and cytochrome c release, the levels of Bax and cytochrome c in the cytosol and mitochondria...
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Tài liệu Báo cáo khoa học: Relationships between structure, function and stability for pyridoxal 5¢-phosphate-dependent starch phosphorylase from Corynebacterium callunaeas revealed by reversible cofactor dissociation studies doc

Tài liệu Báo cáo khoa học: Relationships between structure, function and stability for pyridoxal 5¢-phosphate-dependent starch phosphorylase from Corynebacterium callunaeas revealed by reversible cofactor dissociation studies doc

... corresponding pH-rate profiles. The pKavalues of the cofactor phosphate in unligandedEcMalP and the EcMalP–arsenate complex are 5.6 [26] and 6 .7 [27], respectively. The pKa of the 5¢-phosphategroup ... in the presence of 5 mMpotassium phosphate and potassium sulphate.Reconstitutions with PLP of apo-CcStP and mutantsthereof, and characterization of the wild-typeholo-enzymeIncubation of ... formation of an a po-oligomer induced by the b inding of the nucleotide sugar. The presence of maltopentaose (5 mM) resulted in a moderate 1.5-foldincrease in the half-life of apo-CcStP.Effects of...
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Báo cáo khoa học: Relationship between functional activity and protein stability in the presence of all classes of stabilizing osmolytes ppt

Báo cáo khoa học: Relationship between functional activity and protein stability in the presence of all classes of stabilizing osmolytes ppt

... [32,37,38]. The variation in the effect of stabilizing osmolytes inmodulating the catalytic efficiency of RNase-A in the presence of each class of osmolyte may best beexplained by the combination of ... unfavourableinteraction between the osmolyte and the peptide back-bone, and (b) the total contribution of side chains to the stability of the native state, which may interactdifferently with different osmolytes, ... those in the absence of sugars, i.e. Kmis decreased. On the otherhand, this change in conformation results in a decreasein kcat, the turnover number of the enzyme in the pres-ence of sugars,...
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Báo cáo khoa học: Interactions between coenzyme B12 analogs and adenosylcobalamin-dependent glutamate mutase from Clostridium tetanomorphum pot

Báo cáo khoa học: Interactions between coenzyme B12 analogs and adenosylcobalamin-dependent glutamate mutase from Clostridium tetanomorphum pot

... presence of 10, 25, 50, 75 and 120 lm cofactor, and the Km and kcatfor AdoCbi-GDPwere determined in the presence of 20, 70, 100, 150 and 200 lm cofactor. The reaction was initiated by addingl-glutamate ... energy of the cofactoris sensitive to changes in the pKa of the lower axialbase [3]. This has led to speculation that proteinsmight modulate the pKa of the histidine via the hydrogen bond between ... weakened within the enzyme active site [18,19]. The correlation between the distortion of the adenosyl group and cleavage of the cobalt–carbon bond is still not clear.Although the precise mechanism...
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Báo cáo khoa học: Interaction between Lim15/Dmc1 and the homologue of the large subunit of CAF-1 – a molecular link between recombination and chromatin assembly during meiosis pot

Báo cáo khoa học: Interaction between Lim15/Dmc1 and the homologue of the large subunit of CAF-1 – a molecular link between recombination and chromatin assembly during meiosis pot

... overhang is formed by exonu-clease activity and invades the homologous double-stranded region of the other allele. These steps of homology search and recombination are catalysed by two bacterial ... place between homologous chromosomes of maternal and paternal origin. This is followed by the segregation of maternal and paternal copies of each chromosome. A physical connection at the site of homologous ... the beads with 20 lL of buffer F (50 mm glycine/HCl, pH 2.5, and 0.01%Triton X-100). After neutralization of the pH by the addition of 1 m Tris/HCl, pH 7.5, the bound material was analysed by immunoblotting...
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Báo cáo khoa học: Interactions between metals and a-synuclein ) function or artefact? pptx

Báo cáo khoa học: Interactions between metals and a-synuclein ) function or artefact? pptx

... bemediated by interaction of Cu with the C-terminus of the protein. This was shown by limited proteolysis of the a-synuclein that cleaved off part of the C-terminus,either at residue 97 or 114. The ... [18,44–52]. The mice either expressed human wild-type a-synuclein or the human protein carrying one of the two mainmutants (A53T or A30P). These mice differ in the level of expression of the protein and ... expression of the human proteinin the substantia nigra and, after 13 weeks, the research-ers observed a 50% reduction in the number of dopam-inergic neurones. Unlike other models, the progressionof...
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Báo cáo khoa học: Adenine nucleotides inhibit proliferation of the human lung adenocarcinoma cell line LXF-289 by activation of nuclear factor jB1 and mitogen-activated protein kinase pathways doc

Báo cáo khoa học: Adenine nucleotides inhibit proliferation of the human lung adenocarcinoma cell line LXF-289 by activation of nuclear factor jB1 and mitogen-activated protein kinase pathways doc

... indi-cate a number of links between the activation of p38kinase and the DNA checkpoint pathways and theirpossible interaction in the modulation of cell cycle con-trol and DNA mismatch repair ... 2 and of p50NF-jB1 to the nucleus suggests a role of both proteinsin the regulation of cell cycle progression by extracellu-lar nucleotides. Sustained activation of the ERK1 ⁄ 2 and PI3K pathways ... in epithelial cellsvia activation of NF-jB. Attenuation of LXF-289 cellproliferation by ATP is mediated by the activation of the MEK ⁄ ERK1 ⁄ 2, PI3K and p38 MAPK pathways. Activation and translocation...
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