... [32,37,38]. The variation in the effect of stabilizing osmolytes inmodulating the catalytic efficiency of RNase-A in the presence of each class of osmolyte may best beexplained by the combination of ... unfavourableinteraction between the osmolyte and the peptide back-bone, and (b) the total contribution of side chains to the stability of the native state, which may interactdifferently with different osmolytes, ... those in the absence of sugars, i.e. Kmis decreased. On the otherhand, this change in conformation results in a decreasein kcat, the turnover number of the enzyme in the pres-ence of sugars,...