Báo cáo khoa học: The 3¢-UTR of the mRNA coding for the major protein kinase C substrate MARCKS contains a novel CU-rich element interacting with the mRNA stabilizing factors HuD and HuR ppt
Báo cáo khoa học: The 3¢-UTR of the mRNA coding for the major protein kinase C substrate MARCKS contains a novel CU-rich element interacting with the mRNA stabilizing factors HuD and HuR ppt
... TCT TTC TTT CTT TCT TTC TTT TTT TTT TTC TCG AGC CCC-3¢;antisense:5¢-GGG GCT CGA GAA AAA AAA AAAGAAAGAAAGAAAGAAAGAAAGAAA GAA AGA AAG AAA GAA AGG AAT TCG GGC CCG GGG-3¢) representing base pairs ... for the major protein kinase C substrate MARCKS contains a novel CU-rich element interacting with the mRNA stabilizing factors HuD and HuR Georg Wein 1 , M...
Ngày tải lên: 08/03/2014, 08:20
Báo cáo khoa học: SAF-3, a novel splice variant of the SAF-1/MAZ/Pur-1 family, is expressed during inflammation pptx
... splice acceptor 1A 75 ATCTTCCAGgtaacaac 625 cacctcagGGTCACGCC 1C 87 CCATTCCAGgtgagtag 84 ctccgcagGCCGCGCCG 2 851 CTTCTCCCGgtgtgcac 403 gtccccagGCCGGATCA 364AATGTGAGgtaggaag 277 ctcctcagAAATGTGAG 4 ... nucleic acid and predicted amino acid sequences of the cDNA encod- ing SAF-3 are shown. The initiator ATG codon and stop codon are indicated. Transcription factor SAF-3 is expresse...
Ngày tải lên: 07/03/2014, 02:20
Báo cáo khoa học: Calpain 3: a key regulator of the sarcomere? pot
... substrates. Through the absence of cleavage of these substrates, calpain 3 deficiency leads to abnormal sarcomeres, impairment of muscle contractile capacity, and death of the muscle fibers. These data indicate a ... Kroupova I, Vondracek P, Marikova T, Mazanec R, Zamecnik J, Stanek J, Havlova M & Fajkusova L (2004) Mutations in Czech LGMD 2A patients revealed by analysis...
Ngày tải lên: 23/03/2014, 10:21
Báo cáo khoa học: N-Glycan structures of squid rhodopsin Existence of the a1–3 and a1–6 difucosylated innermost GlcNAc residue in a molluscan glycoprotein pot
... of the Japan Science and Technology Corporation, the Yamada Science Foundation, and the Mizutani Foundation for Glycoscience. K.K.H. and H.H.H. are supported financially by the Academia Sinica, ... which was confirmed by cochro- matography of the digestion product of glycan B1 with glycan C on the ODS and amide-silica columns. On the basis of these data, we c...
Ngày tải lên: 23/03/2014, 17:22
Báo cáo khoa học: Structure and function of the 3-carboxy-cis,cis-muconate lactonizing enzyme from the protocatechuate degradative pathway of Agrobacterium radiobacter S2 pdf
... C- terminal His-tagged enzyme, as follows [23]. The DNA segments encompassing pcaB1S2 were amplified by PCR using the primers CMLEI-X-N (ATA ACA TAT GAG CCT TTC CCC CTT CGA AC) and CM LEI-His -C (AAA ... in the protoca- techuate branch of the 3-oxoadipate pathway of various aerobic bacteria. The gene encoding a 3-carboxy-cis,cis-muconate lactonizing enzyme (pcaB1S2) was clo...
Ngày tải lên: 30/03/2014, 10:20
Tài liệu Báo cáo khoa học: Glycomics-based analysis of chicken red blood cells provides insight into the selectivity of the viral agglutination assay docx
... completed additional MS and NMR-based analysis of the cRBC glycan pool. In addition to identification and quantification of other monosaccharides, we aimed to characterize the overall sialic acid content, ... GlcNAc-5, GlcNAc-5¢, galactose (Gal)-6, Gal-6¢ and Gal-8 appear in the range 4.40–4.75 p.p.m. (Table S1). Specifically, the anomeric proton of GlcNAc-2 appears at 4.62 p...
Ngày tải lên: 14/02/2014, 19:20
Tài liệu Báo cáo khoa học: 3T3-L1 adipocyte apoptosis induced by thiazolidinediones is peroxisome proliferator-activated receptor-c-dependent and mediated by the caspase-3-dependent apoptotic pathway doc
... 693 TTTTTCAGTGCAGAA-3¢; aP2 forward, 5¢-AAAGACA GCTCCTCCTCGAAGGTT-3¢; and aP2 reverse, 5¢-TGA CCAAATCCCCATTTACGC-3¢. Standard curves were gen- erated with 10-fold serial dilutions ranging from ... against actin in the same sample. The PCR primers were as follows: actin forward, 5¢-GA AATCGTGCGTGACATCAAAG-3¢; actin reverse, 5¢-TG TAGTTTCATGGA TGCCACAG-3¢; Akt-1 forward, 5¢ -A ACGGACTT...
Ngày tải lên: 16/02/2014, 09:20
Tài liệu Báo cáo khoa học: Minor capsid proteins of mouse polyomavirus are inducers of apoptosis when produced individually but are only moderate contributors to cell death during the late phase of viral infection ppt
... caspase 3 activity and the caspase inhibition assay At indicated time-points, cell lysates were prepared and tested for cleavage of amino acid DEVD sequences by cas- pase 3 using the CaspACE assay system, ... polyoma- virus capsid proteins that serve as nuclear localization signals. Trans Kans Acad Sci 96, 35–39. 5 Moreland RB & Garcea RL (1991) Characterization of a nucle...
Ngày tải lên: 16/02/2014, 09:20
Tài liệu Báo cáo khoa học: An autoinhibitory effect of the homothorax domain of Meis2 ppt
... 5¢-GAGGTCGATGGGCATTTTC-3¢; Meis3-F, 5¢-GATGATCCAGCCATCCA-3¢; Meis3-R, 5¢- GGCTGGGTAGTCCTCGAAGT-3¢; mMeis3-F, 5¢-GTCC AGGCCATCCAGGTACT-3¢; and mMeis3-R, 5¢-TCCTCC CTGCAACTACCATC-3¢. The relative ... the conformation or intramolecular interactions, allowing access to the AD. As the autoinhibition affected an unrelated AD when this was put in place of the native Meis2d AD, it appea...
Ngày tải lên: 16/02/2014, 15:20
Tài liệu Báo cáo khoa học: NMR structural characterization of HIV-1 virus protein U cytoplasmic domain in the presence of dodecylphosphatidylcholine micelles doc
... interior of the micelle or the aqueous buffer. Qualitative analysis of secondary chemical shift and paramagnetic relaxation enhancement data in conjunction with dynamic information from heteronuclear ... and the chemical shift list reflecting the sequence-speci c resonance assign- ment. Intermediate protein structures are calculated at the end of each cycle and provi...
Ngày tải lên: 18/02/2014, 06:20