... slope of the curve found when the medium did not contain AdoMet, with an estimated half-life of 10.5 min. The role of the seven cysteine residues in L. donovani MAT was studied using the site-directed ... located in the central domain of eachsubunit, in the interface between the two dimers compri-sing the tetrameric structure [29]. This domain containsfive cysteine residues, two of them (Cys35 ... todetermine the functional role of the amino acid residues involved in each. The present report describes the cloningand subsequent functional refolding, using a bacterialexpression system, of the...