Báo cáo khoa học: Leishmania donovani methionine adenosyltransferase Role of cysteine residues in the recombinant enzyme pptx

... slope of the curve found when the medium did not contain AdoMet, with an estimated half- life of 10.5 min. The role of the seven cysteine residues in L. donovani MAT was studied using the site-directed ... located in the central domain of each subunit, in the interface between the two dimers compri- sing the tetrameric structure [29]. This domain contai...

Ngày tải lên: 08/03/2014, 08:20

... lacks residues 1–174 of the N-terminal domain and retains full enzyme activity in vitro. The enzyme contains a central DNA-binding core domain and a C-terminal catalytic domain harboring an SKINYL ... recon- stitution of the two recombinant proteins LdTOPIL and LdTOPIS, corresponding to the large and small subunits, and localization of the active enzyme in both...

Ngày tải lên: 30/03/2014, 10:20

... positioning of model residues in unusual areas of the Ramachandran plot. The altered main chain conformation in the vicinity causes the Gln287 side chain to intrude into the area corresponding to the ... differences in the residues involved in nucleotide binding and three differences in the residues involved in the binding of fructose 6-phosphate. Th...

Ngày tải lên: 23/03/2014, 21:21

... of the pro- tein in the N state and the evolution of the overall structure of the protein in the MG state. This degree of resolution is unprecedented for the T domain of the diphtheria toxin. ... chain corresponding to the catalytic domain (C domain); and the B chain corresponding to the translocation domain (T domain) and the receptor-binding domain. Th...

Ngày tải lên: 16/02/2014, 09:20

... regulates the flow of the Gibbs free energy inherent in C–C bonds in fatty acids into the production of either chemical energy or heat by directing the flow of iPLA 2 c into eith er the peroxisomal ... profiles and protein chemical techniques including radiolabeling with [ 3 H]BEL [7–12]. Study of human heart PLA 2 underscored the complexity of multiple distinct isoform...

Ngày tải lên: 19/02/2014, 16:20

... Predicting the fate of anglicisms We examine here factors hypothesized to play a role in the establishment (or decline) of anglicisms. Frequency in the English Covo corpus We first examine whether ... portion of the MZEE cor- pus, the training data consisted of the disjoint sub- sets of the English and German CELEX wordlists (Baayen et al., 1995), as well as the...

Ngày tải lên: 19/02/2014, 19:20

... gene. The underlined amino acid residues are the signal sequences of the respective genes. The asterisk indicates the residues important for an interaction with the IL-10R/R1 receptor chain. The ... conserved cysteine residues in human IL-10 and IL-22 were found to be conserved in carp IL-10. The first cysteine forms a disulphide linkage with the third cystei...

Ngày tải lên: 20/02/2014, 02:21

... the remaining b-strands, with the exception of strand 6, are arranged in two antiparallel beta sheets within the a-crystallin domain. The inter- face between monomers of a dimer involves interaction between ... directly in the dimer–dimer interface. As a result of the spatial dis- position of monomers and their b-strand elements in the a-crystallin domain, the am...

Ngày tải lên: 07/03/2014, 12:20

... c 10 complex in the recombinant c ring preparations suggests that the insertion of the last c subunit forms the limiting step in the assembly process of a functional oligomer. Upon closer inspection, the ... to clarify whether the stoichiometry of the c rings is in uenced by variation of the expression level of subunit c. In the present study we investig...

Ngày tải lên: 07/03/2014, 21:20

... demonstrated that the serine protease inhibitor a-1 antitrypsin binds preprohepcidin within the cell during maturation. Furthermore, a-1 antitrypsin binds prohepcidin significantly in the plasma. This ... hepcidin is with the iron exporter molecule ferroportin. We were interested in whether we could identify new protein– protein interactions of preprohepcidin, prohepcidin and hep...

Ngày tải lên: 16/03/2014, 01:20