Báo cáo khoa học: "Role of Verbs in Document Analysis" pot

Tài liệu Báo cáo khoa học: Role of Kupffer cells in pathogenesis of sepsis-induced drug metabolizing dysfunction pptx

... shown that interactions betweenKCs and endotoxin comprise the initiating event lead-ing to hepatotoxicity in liver injury, including endotox-emia and ischemia ⁄ reperfusion injury [14]. In ourstudies, ... mediator of lethal sepsis, triggering theproduction of proinﬂammatory cytokines and liverinjury [13].Therefore, the present study aimed to elucidate therole of KCs in the regulation of CYP isoform ... [22]. ROS indirectly reduced the activity of selected isoforms of CYP by inducing phosphorylation of the isoforms [23]. Activated KCs cause oxidativestress on the surrounding tissue, releasing largeamounts...

Tài liệu Báo cáo khoa học: Role of the cag-pathogenicity island encoded type IV secretion system in Helicobacter pylori pathogenesis pptx

... have indi-cated that the CagAPY-induced cell elongation pheno-type also involves tyrosine dephosphorylation of cortactin, vinculin and ezrin, which are three well-known actin-binding proteins ... phosphatasesinvolved in this scenario, however, remain unknownand do not require Shp2. Instead, CagAPYcan inhibitSrc activity both by direct interaction of both proteinsor by binding of CagAPYto ... of distinctEPIYA-independent domains within CagA that playessential roles in protein targeting and alteration of host-cell transcription signalling pathways.Another important consequence of...

Tài liệu Báo cáo khoa học: Role of ceramide kinase in peroxisome proliferatoractivated receptor beta-induced cell survival of mouse keratinocytes ppt

... role in skin woundhealing. After a skin injury, PPARb expression is rap-idly elevated in the epidermis at the wound edges; dele-tion of a single PPARb allele results in delayed woundhealing ... culturedhuman keratinocytes [14]. In this study, we examined the interaction betweenCerK and PPARb, and its role in regulating keratino-cyte survival. We report that in a mouse keratinocytecell line upregulation ... role in keratinocyte sur-vival during skin wound healing, and PPARb expres-sion is elevated at injury sites [10,15]. We investigatedwhether skin barrier disruption by tape-strippingwould induce...

Tài liệu Báo cáo khoa học: Role of receptor-mediated endocytosis, endosomal acidiﬁcation and cathepsin D in cholera toxin cytotoxicity pdf

... action of cholera toxin in rat liver parenchyma. Following administration of a saturating dose of cholera toxin to rats, rapid endocytosis of both cholera toxin subunits wasobserved, coincident ... acidic cath-epsin D in promoting the cytotoxic action of CT, mostprobably through the removal of C-terminal residues of CT-A1 encompassing the terminal KDEL peptide.Our in vivo and in vitro studies ... cathepsin D inhibitor pepstatin Apartially inhibited, both in vivo and in vitro, the cAMP response to choleratoxin. This cathepsin D-dependent action of cholera toxin under the con-trol of endosomal...

Tài liệu Báo cáo khoa học: Role of transcription factor activator protein 1 (AP1) in epidermal growth factor-mediated protection against apoptosis induced by a DNA-damaging agent doc

... transcription, were increased in response toEGF. Pretreatment of the cells with PD98059, an inhibitor of MAP kinasekinase, inhibited the EGF-induced c-Fos and c-Jun expression, AP1 DNAbinding, Bcl-XLexpression, ... vector by using Lipofectamine (Invitrogen).Cells were selected in neomycin, and clones were isolatedand screened for Bcl-XLexpression.Creation of TAM67-overexpressing cell lineThe dominant-negative ... protein-tyrosine kinases in vivo and in vitro. Cell 57, 1109–1122.18 Holgado-Madruga M, Emlet DR, Moscatello DK,Godwin AK & Wong AJ (1996) A Grb2-associateddocking protein in EGF- and insulin-receptor...

Tài liệu Báo cáo khoa học: Role of K22 and R120 in the covalent binding of the antibiotic fosfomycin and the substrate-induced conformational change in UDP-N-acetylglucosamine enol pyruvyl transferase docx

... notinteract with R124 in the ternary complex [20]. Hence, themechanism of binding of fosfomycin by reinforcement of aninitial binding site (mainly K22 and R397) through recruit-ment of secondary ... charged amino acid sidechains, R397 and R120, are also involved in fosfomycinbinding, and in view of the multitude of interactions, it wasassumed that deletion of a single interaction would not ... chain of K22 participates in fosfomycin binding, thus providing a rationale for the loss of fosfomycin binding to the K22V and K22E mutant proteins.However, two other positively charged amino...

Tài liệu Báo cáo khoa học: Role of hydroxyl group and R/S conﬁguration of isostere in binding properties of HIV-1 protease inhibitors docx

... onconformation of inhibitor main chain. Thus, differentinhibitors differ not only in t he binding afﬁnity, bu t a lso in the degree o f freedom of the inhibitor inside the bindingtunnel. Some s pecial inhibitors ... Phe53 rings are in both o rientationsstacked by p–p interaction with Phe153 rings of theneighbouring protein dimer in crystal. The alternativepositions of phenylalanine rings a re formed in concert ... 271) 4453Inhibitor binding. The inhibitor OE b inds into the p roteasebinding tunnel in an e xtended c onformation in two oppositedirections (Fig. 4). The ﬂaps are locked over the inhibitor...

Tài liệu Báo cáo khoa học: Role of cleavage and shedding in human thyrotropin receptor function and trafﬁcking pdf

... periods of times (0–60 min)at 37 °C. The concentration of the biotinylated Ig remainingon the cell surface was then quantiﬁed by measuring thebinding of [125I]Streptavidin. In the absence of ... Theywere then incubated with 0.25 mL of incubation mediumcontaining 2 lgÆmL)1 of biotinylated R5T-34 monoclonalIg [30] at 4 °C for 30 min. In some cases, monensin 40 lMwas added to the incubation ... measuring thebinding of [125I]Streptavidin [30]. No Ig binding wasobserved in cells transfected with the vector alone (Fig. 4B). As shown in Fig. 4B, quantiﬁcation of cell surfaceexpression of...

Báo cáo khoa học: Role of the C-terminal extension in a bacterial tyrosinase Michael Fairhead and Linda Thony-Meyer doc

... tyrosinase 320 amino acidsCore domain 36–357Copper binding motifArg40Cys84Tyr349Tyr347Copper binding motifTrypsinisedpro-tyrosinase 332 amino acidsCore domain 36–370Copper binding ... motifArg40Cys84Tyr349Tyr347Lys370Ala36Ala36Ala36Val357Phe518BLaccaseTyrosinaseβ-sheet proteinFig. 1. Overview of the tyrosinase geneand surrounding genes in the genome of V. spinosum. (A) Showing the tyrosinasegene and those in its immediate vicinity in the ... kDa) assuming the removal of theN-terminal methionine.Reconstitution of recombinat V. spinosumtyrosinase with copperThe holo-forms of tyrosinase were obtained after puri-ﬁcation by adding copper...

Báo cáo khoa học: Role of DptE and DptF in the lipidation reaction of daptomycin ppt

... in DptF is GLDSV,indicating that this putative ACP domain is one of thefew ACPs in which valine replaces isoleucine (I) orleucine (L) in the conserved sequence. To determinewhether DptF is ... remains to be clariﬁed whether all of the fatty acids activated by DptE can be incorpo-rated into the ﬁnal product or whether there is aninterfering speciﬁcity of the CIIIdomain of the initia-tion ... of DptE, weexpressed dptF using the pQTev vector in E. coli andpuriﬁed the resulting ACP as an N-terminal His7 fusionprotein (Fig. 3) with a yield of 9.5 mgÆL)1 of culture.The identity of...

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