Báo cáo khoa học: "Role of Verbs in Document Analysis" pot

... shown that interactions between KCs and endotoxin comprise the initiating event lead- ing to hepatotoxicity in liver injury, including endotox- emia and ischemia ⁄ reperfusion injury [14]. In our studies, ... mediator of lethal sepsis, triggering the production of proinflammatory cytokines and liver injury [13]. Therefore, the present study aimed to elucidate the role of KCs in the...

Ngày tải lên: 14/02/2014, 18:20

... have indi- cated that the CagA PY -induced cell elongation pheno- type also involves tyrosine dephosphorylation of cortactin, vinculin and ezrin, which are three well- known actin-binding proteins ... phosphatases involved in this scenario, however, remain unknown and do not require Shp2. Instead, CagA PY can inhibit Src activity both by direct interaction of both proteins or by binding...

Ngày tải lên: 14/02/2014, 19:20

... role in skin wound healing. After a skin injury, PPARb expression is rap- idly elevated in the epidermis at the wound edges; dele- tion of a single PPARb allele results in delayed wound healing ... cultured human keratinocytes [14]. In this study, we examined the interaction between CerK and PPARb, and its role in regulating keratino- cyte survival. We report that in a mouse ker...

Ngày tải lên: 18/02/2014, 18:20

... action of cholera toxin in rat liver parenchyma. Following administration of a saturating dose of cholera toxin to rats, rapid endocytosis of both cholera toxin subunits was observed, coincident ... acidic cath- epsin D in promoting the cytotoxic action of CT, most probably through the removal of C-terminal residues of CT-A1 encompassing the terminal KDEL peptide. Our in v...

Ngày tải lên: 19/02/2014, 02:20

... transcription, were increased in response to EGF. Pretreatment of the cells with PD98059, an inhibitor of MAP kinase kinase, inhibited the EGF-induced c-Fos and c-Jun expression, AP1 DNA binding, Bcl-X L expression, ... vector by using Lipofectamine (Invitrogen). Cells were selected in neomycin, and clones were isolated and screened for Bcl-X L expression. Creation of TAM67-overexpr...

Ngày tải lên: 19/02/2014, 06:20

... not interact with R124 in the ternary complex [20]. Hence, the mechanism of binding of fosfomycin by reinforcement of an initial binding site (mainly K22 and R397) through recruit- ment of secondary ... charged amino acid side chains, R397 and R120, are also involved in fosfomycin binding, and in view of the multitude of interactions, it was assumed that deletion of a sing...

Ngày tải lên: 19/02/2014, 13:20

... on conformation of inhibitor main chain. Thus, different inhibitors differ not only in t he binding affinity, bu t a lso in the degree o f freedom of the inhibitor inside the binding tunnel. Some s pecial inhibitors ... Phe53 rings are in both o rientations stacked by p–p interaction with Phe153 rings of the neighbouring protein dimer in crystal. The alternative positions of ph...

Ngày tải lên: 19/02/2014, 16:20

... periods of times (0–60 min) at 37 °C. The concentration of the biotinylated Ig remaining on the cell surface was then quantified by measuring the binding of [ 125 I]Streptavidin. In the absence of ... They were then incubated with 0.25 mL of incubation medium containing 2 lgÆmL )1 of biotinylated R5T-34 monoclonal Ig [30] at 4 °C for 30 min. In some cases, monensin 40 l M was a...

Ngày tải lên: 21/02/2014, 00:20

... tyrosinase 320 amino acids Core domain 36–357 Copper binding motif Arg40 Cys84 Tyr349 Tyr347 Copper binding motif Trypsinisedpro-tyrosinase 332 amino acids Core domain 36–370 Copper binding ... motif Arg40 Cys84 Tyr349 Tyr347 Lys370 Ala36 Ala36 Ala36 Val357 Phe518 B Laccase Tyrosinase β -sheet protein Fig. 1. Overview of the tyrosinase gene and surrounding genes in the genome of...

Ngày tải lên: 06/03/2014, 11:20

... in DptF is GLDSV, indicating that this putative ACP domain is one of the few ACPs in which valine replaces isoleucine (I) or leucine (L) in the conserved sequence. To determine whether DptF is ... remains to be clarified whether all of the fatty acids activated by DptE can be incorpo- rated into the final product or whether there is an interfering specificity of the C III domain of...

Ngày tải lên: 07/03/2014, 04:20