... thioester acyl–enzymeintermediate. Biochemistry 49, 341–346.3 Fukuda A, Matsuyama S, Hara T, Nakayama J,Nagasawa H & Tokuda H (2002) Aminoacylation of the N-terminal cysteine is essential for ... than that from RN4220, MW2 or MSSA476 strain, indicatingthat shorter fatty acids were mainly attached to the tria-cylated lipopeptides of SitC of the SA113 strain(Figs 1A and 3). The usage of ... strain wasreported to be diacylated [11], we then asked whether the SitC lipoproteins of three other strains of S. aur-eus, including SA113 strain, and of S. epidermidisATCC12228 strain are...