Báo cáo khoa học: Systematic characterization of mutations in yeast acetohydroxyacid synthase doc

... case of CS. Since this side chain of CE makes important interactions with the protein, it is possible that the binding mode of other sulfonylureas differs from that of CE. Fig. 1. Pathway of branched-chain ... Duggleby et al. (Eur. J. Biochem. 270) Ó FEBS 2003 Systematic characterization of mutations in yeast acetohydroxyacid synthase Interpretation of herbicide-re...

Ngày tải lên: 08/03/2014, 02:21

... 2A). In some cells, the mutant proteins were also observed in the cytoplasm, as seen for the wild-type protein. Similar staining patterns were observed in other human cell lines, including hepatoma ... the presence and absence of 0.25 m M galactose was determined by measuring BrdU incorporation. The level of BrdU incorporation into each cell line cultured in the absence of galac...

Ngày tải lên: 07/03/2014, 00:20

... proteins are a large family of RNA-binding proteins found in all eukaryotes. These proteins reduce the expression of mRNA targets by binding in 3¢ UTR regulatory elements, thus controlling translation ... predict that various PUF of A. thalina may be involved in many different processes in the plant. RNA-binding capacity of the APUM proteins pfam analysis of all putative APU...

Ngày tải lên: 18/02/2014, 06:20

... concentration of the 16 kDa protein subunit) increased linearly with increasing fractional saturation of the metal site in Bxe_A2876, the k cat determined from the molar concentration of Fe 2+ -containing ... one of the following substrates was offered to a protein solution containing 7 lm metal sites: xanthine in the presence of 2-ketoglutarate; cat- echol; or quercetin. Howeve...

Ngày tải lên: 18/02/2014, 06:20

... stock of 0.5 nm radiolabeled 5¢ exon mimic in H10Mg buffer at 44 °C was added. The mixture was incubated at 44 °C for at least 90 min. To maintain the integrity of the bound species during gel ... site can play a role in determining the binding regis- ter of reaction substrates. Biochemistry 44, 1067–1077. P. P. Dotson II et al. Kinetics of the trans excision-splicing reaction FEB...

Ngày tải lên: 18/02/2014, 18:20

... (a-1 in Fig. 3), indicating that in- plane anisotropy of heme is relatively large. In addition, small amounts of low-spin species are also observed in the neutral solution, as distinctly seen in ... gradual diminution of the Soret band. After several minutes, a broad band appears at around 660–675 nm; this increases in inten- sity with time, indicating the formation of bili...

Ngày tải lên: 19/02/2014, 05:20

... freely inter- converting substates, one of which corresponds to a folded state that is binding-competent. In the former model, binding precedes folding, and in the latter, fold- ing precedes binding. ... and so binding is coupled to induction of the structure of the N-domain. Measurements in the presence of the osmolyte, trimethylamine N-oxide, which induces the structure of u...

Ngày tải lên: 19/02/2014, 05:20

... using crotonyl-CoA as a substrate [15]. The gene encoding this bifunctional protein was named AUH (‘AU bind- ing homolog of enoyl-CoA hydratase’). The RNA- binding activity of the human protein ... localisa- tion of AUH, an AU-specific RNA-binding enoyl-CoA hydratase. Gene 228, 85–91. 17 Nakagawa J & Moroni C (1997) A 20-amino-acid autonomous RNA-binding domain contained in an enoy...

Ngày tải lên: 19/02/2014, 07:20

... 235 [9]. Of theses residues, only Arg213 is in the vicinity of the active site of RTA. In order to establish the role of arginyl residues around the active site of RTA in the binding of rRNA, ... expression. Ricin is a potent toxin found in the seeds of the Ricinus communis plant. It is a member of a large family of ribosome-inactivating proteins (RIPs) that exist i...

Ngày tải lên: 19/02/2014, 12:20

... and anaerobic condi- tions, in the range of 0.6 lmolÆmg )1 Æmin )1 . No evidence was obtained for redox cycling of FMN. The amino acid residues involved in the FMN binding site are absolutely conserved ... M15-pQEidi2. Preparation of the recombinant Idi-2 protein The recombinant E. coli strain M15-pQEidi2 was grown in Luria-Bertani broth containing ampicillin (180 mgÆL )1 )and kan...

Ngày tải lên: 19/02/2014, 13:20