Báo cáo khoa học: Selectivity of pyruvate kinase for Na+ and K+ in water/dimethylsulfoxide mixtures docx

... was initiated by introducing pyruvate kinase. Fluorescence experiments Fluorescence emission spectra of pyruvate kinase in 100% water and in various water/dimethylsulfoxide mixtures were determined ... activities of pyruvate kinase with Na + and K + could be related to distinct structural features of the enzyme. Therefore, we determined the intrinsic fluorescence...

Ngày tải lên: 08/03/2014, 02:20

... generated, making multiple isoforms in combi- nation [4]. Among these isoforms, DNp63a is the predominant isoform expressed during embryogenesis and in adult epidermal tissues, and is responsible for epidermal ... degradation of DNp63a by TNF-a was attenuated by inhibition of NF-jB, sug- gesting that activation of NF-jB may be involved in the regulation of the degradation...

Ngày tải lên: 16/03/2014, 06:20

... affected in a similar way. Electrostatic interactions play a critical role in guiding and stabilizing functional protein–pro- tein interactions [6], as well as in the structural stability of proteins ... provided important insights into the role of heme–protein interactions for the structural stability of this class of proteins. The mutant D72K of cytochrome c 6 from Nosto...

Ngày tải lên: 30/03/2014, 20:20

... cytoplasmic mem- brane. These findings indicate that inhibition of NifA in the presence of oxygen and/ or ammonium occurs via direct NifL interaction and formation of those inhibitory NifL– NifA complexes ... tetracycline-resistance cassette into the HindIII site. pRS239 was obtained by inserting the tetra- cycline-resistance cassette into the HindIII site of pRS155, encoding glnK...

Ngày tải lên: 07/03/2014, 16:20

... Westrick RJ & Ginsburg D (1998) Comparative mapping of distal murine chro- mosome 11 and human 17q21.3 in a region containing a modifying locus for murine plasma von Willebrand factor level. ... encodes a region of the protein that is known to be essential for kinase activity and contains a diacylglycerol kinase- like catalytic domain [2]. As shown in Fig. 5A, cell deat...

Ngày tải lên: 18/02/2014, 18:20

... membrane expansion and remodeling. In most instances, there is coordinate expression of the E1 and E2 components. The transient accumulation of mRNA preceding changes in protein levels and catalytic ... expression of mtPDC among dicot plants. In a detailed study of pea (Pisum sativum) seedling development, it was observed that changes in the levels of E1 and E2 prote...

Ngày tải lên: 17/03/2014, 09:20

... measurements of the interaction of IVIg with C-reactive protein (CRP). The binding profiles obtained after single injections of native and of low-pH buffer-exposed IVIg were com- pared. As shown in Fig. ... Increased binding of low-pH buffer-exposed IVIg to human factor H. In both panels, binding of the native IVIg is indicated by a solid line, and that of the low-pH buff...

Ngày tải lên: 16/02/2014, 15:20

... 3729–3734. Supporting information The following supplementary material is available: Fig. S1. Amino acid alignment of linker region and ligand-binding domain (LBD) of ecdysone receptors. Fig. S2. Amino acid ... nonsteroidal agonists bind exclusively to the EcR ligand-binding domain (LBD), although the presence of USP increases ligand-binding affinity [12–15]. The diversity of ligand-...

Ngày tải lên: 18/02/2014, 08:20

... FEBS analysis of purine-binding proteins in a total lysate of human Jurkat T-cells [40]. COFRADIC analysis of protein processing ) protease degradomics Protein processing introduces novel protein fragments that ... light 12 C 6 -arginine form. Almost identical numbers of pre-existing N-ter- mini (i.e. the N-termini of intact proteins), on the other hand, should appear as couples...

Ngày tải lên: 18/02/2014, 16:20

... °C, and carried out for 32 cycles of 94 °C for 30 s, annealing temperature for 1 min and 30 s and 72 °C for 30–90 s. Products were visual- ized by ethidium bromide in agarose gels, and bands ... the main nitrogen compounds in the phloem exudates, amounting to 51% of the total amino acids in the light and 56% in the dark (Fig. 7A). The total amount of alanine, s...

Ngày tải lên: 18/02/2014, 18:20