... protein degradation. Proc Natl
Acad Sci USA 98, 1012–1017.
17 Sakata E, Yamaguchi Y, Kurimoto E, Kikuchi J,
Yokoyama S, Yamada S, Kawahara H, Yokosawa H,
Hattori N, Mizuno Y et al. (2003) Parkin binds ... Institute of Scientific and Industrial Research, Osaka University, Japan
2 Laboratory of Industrial Biosciences, Graduate School of Bioagricultural Sciences, Nagoya University, Japan
3...
... broad
implications, as a pathway that involves phospholipase A
2
,
hydroxyl radical, guanylate cyclase, and PKG has been
mapped to T-cell signal transduction leading to apoptosis [3]
as well as ... proteins by cyclic GMP-dependent kinase
A noncanonical pathway to NF-jB activation
Bin He
1
and Georg F. Weber
1,2
1
Department of Radiation Oncology, New England...
... -ATC ATC TCC
ATC GAC TAC TCC CTG-3¢, the antisense primer
5¢-AAG AAT
TCT AGA TTA ATG GTG ATG ATG GTG
ATG ATG GTG TGG GGT CAG CGG TGC AGC AGG
GGG GGT-3¢ (XbaI sites underlined; His-tag in italic) ... phospholipids and that this is fol-
lowed by an interaction between the apolar acyl chains
of the phospholipids and side chains of particular
amino acids, thus accounting for the increased c...
... 3¢-GG
AACCACGAAAGTAG CAATC AGA ACACTA AAACC A
GGTACAATGATGC-5 ¢ . All vector inserts were
sequenced prior to use.
CDK4 pY17 antibody generation
Murex lop-eared rabbits were injected with a phosphopep-
tide ... kinase. (A) The ammo-
nium sulfate precipitated HeLa nuclear lysate was fractionated by
butyl-sepharose chromatography and the even numbered frac-
tions were assayed for Y17 k...
... Facchin S, Ruzzene M, Peggion C, Sartori G, Carignani
G, Marin O, Brustolon F, Lopreiato R & Pinna LA
(2007) Phosphorylation and activation of the atypical
kinase p53-related protein kinase ... exponential phase, and then incubated for 30 min in galac-
tose medium to activate the GAL regulon. Total mRNAs were
extracted and subjected to standard northern blot analysis. GAL1
m...
... M, Ito Y,
Shibata T & Yokoyama S (1999) Cell-free production
and stable-isotope labeling of milligram quantities of
proteins. FEBS Lett 442 , 15–19.
3 Madin K, Sawasaki T, Ogasawara T & ... different samples. The last column displays the average
amino acid abundance in proteins according to the NCBI database.
Fig. 2.
15
N-HSQC spectra of five combinatorially
15
N-labelled sa...
... tyrosine kinases that are activated
downstream of the EGF receptor, we regarded Src family
kinases as possible candidates, as these kinases are activated
downstream of all known receptors that mediate ... the
receptor at the acidic endosomal pH, thus activating the
receptor kinase, transforming growth factor a (TGFa)
rapidly dissociates and leaves the EGF receptor inactive
after intern...
... environmental stress,
mRNA metabolism is reprogrammed to adapt to
stress-induced damage. Translationally stalled mRNAs
together with a number of translation initiation factors
and RNA-binding proteins are ... suggested that the SARS-
CoV N protein can act as a substrate of various
kinases, such as cyclin-dependent kinases, glycogen
synthase kinase, creatine kinase II and mito...
... allowed
authors to isolate a variant of a M. thermophila laccase
capable of resisting a wide array of co-solvents at con-
centrations as high as 50% v ⁄ v [14]. In all available
examples of molecular ... FEBS
Active variants of laccase were selected directly on
transformation plates. Expression, purification and
analysis of the pH activity profile allowed the charac-
terizat...
... Kadota J, Yamamoto T, Yoshiuchi S, Bi E & Tanaka
K (2004) Septin ring assembly requires concerted action
of polarisome components, a PAK kinase Cla4p, and
the actin cytoskeleton in Saccharomyces ... in membranes but
also the amount of sterols derived from SE that are
Table 1. Sterol analysis of cells lacking STE20 and CLA4. Data are mean values with standard deviation from at le...