... in vitro refolding pathway of PIP and HPI. (A) Amino acid sequences of PIP and HPI. Amino acids are shown in the one-letter code. The numbering of the residues in HPI and PIP are based on each ... native PAGE. Isolation and purification of the scrambled disulfide isomers of HPI In the presence of denaturant and thiol catalyst as indicated above, HPI was converted into th...
Ngày tải lên: 19/02/2014, 12:20
... Involvement of acetaldehyde in alcohol addiction. Alcohol Clin. Exp. Res. 26, 114–119. 30. Johansson, B. (1992) A review of the pharmacokinetics and pharmacodynamics of disulfiram and its metabolites. ... genes ADH1, ADH3 and ADH4. Similar mRNA length and high nucleotide and amino acid sequence identity of all ADHs lead to a large risk for cross-reactivity of probes at th...
Ngày tải lên: 21/02/2014, 00:20
Báo cáo khoa học: Enzymes of creatine biosynthesis, arginine and methionine metabolism in normal and malignant cells Soumen Bera1, Theo Wallimann2, Subhankar Ray1 and Manju Ray1 potx
... prominent up-regulation of the expression and enzymatic activity of both AGAT and GAMT. The specific activity of AGAT in sarcoma tissue reached almost 50% of that observed in the kidney and it was also ... promote ornithine and SAH production, which in turn could be used for the formation of polyamines and methionine. An investigation of the level of different intermedi...
Ngày tải lên: 07/03/2014, 04:20
Báo cáo khoa học: Isoquinoline-1,3,4-trione and its derivatives attenuate b-amyloid-induced apoptosis of neuronal cells pdf
... [21–24]. Isatin sulfonamide and its ana- logues are potent and selective inhibitors of apoptosis of chondrocytes and mouse bone marrow neutrophils in cell-based models of osteoarthritis [25]. M-791 ... isoquinoline-1,3,4-trione and its derivatives protected the growth of axons and dendrites of neurons treated with Ab(25–35) and attenu- ated neuronal apoptosis. Moreov...
Ngày tải lên: 07/03/2014, 11:20
Báo cáo khoa học: Effect of priming on activation and localization of phospholipase D-1 in human neutrophils potx
... subcellular distribution of PLD and the conse- quences of priming and activation were determined using immunoprecipitation and Western blot analysis of neutro- phil cytosol and membrane fractions ... burst. However, the repertoire of PLD isoforms present in these primary cells, the precise mechanism of activation, and the impact of cell priming on PLD activity and loca...
Ngày tải lên: 07/03/2014, 15:20
Báo cáo khoa học: Adaptation of intronic homing endonuclease for successful horizontal transmission pptx
... published recognition properties of I-SpomI, I-ScaI, and I-SceII were reconsidered from this point of view, and we detected proficient adaptation of I-SpomI, I-ScaI, and I-SceII for target site sequence ... occurs five nt downstream of the intron insertion site on the coding strand and one nt downstream of the insertion site on the noncoding strand, creating 3¢ overhangs of...
Ngày tải lên: 07/03/2014, 17:20
Tài liệu Báo cáo khoa học: DNA methylation-mediated nucleosome dynamics and oncogenic Ras signaling pptx
... regulation of growth and differentiation in eukaryotic cells [1,82]. Despite profound improvements in our understanding of the molecular and cellular mechanisms of action of the Ras proteins, the expanding ... demethylation and hypermethylation. Acknowledgements This review is dedicated (by SKP) to Dr MK Pal, retired professor of biochemistry, University of Kaly- ani and D...
Ngày tải lên: 18/02/2014, 14:20
Tài liệu Báo cáo khoa học: Interruption of triacylglycerol synthesis in the endoplasmic reticulum is the initiating event for saturated fatty acid-induced lipotoxicity in liver cells pdf
... concentrations of stea- rate (18:0), oleate (18:1), or mixtures of the two fatty acids, and the effects on cell proliferation, lipid droplet accumulation and induction of endoplasmic reticulum stress and ... under- stood and need further investigation. A number of recent in vitro and in vivo studies have shown that different forms of fatty acids exert remark- ably d...
Ngày tải lên: 14/02/2014, 22:20
Tài liệu Báo cáo khoa học: Modulation of cyclin D1 and early growth response factor-1 gene expression in interleukin-1b-treated rat smooth muscle cells by n-6 and n-3 polyunsaturated fatty acids pdf
... action of EPA and DHA (Eur. J. Biochem. 271) 4465 Modulation of cyclin D1 synthesis and hyperphosphorylation of Rb by n-3 and n-6 PUFAs Induction of cyclin D1 is one of the e arliest effects of mitogenic ... contribute to the modu- lation of egr-1 gene expression (Fig. 4B). Effects of n-3 and n-6 PUFAs on the synthesis of caveolin-1 and caveolin-3 and on the loca...
Ngày tải lên: 19/02/2014, 16:20
Báo cáo khoa học: Mutants of Saccharomyces cerevisiae deficient in acyl-CoA synthetases secrete fatty acids due to interrupted fatty acid recycling docx
... (square)]. (D) Con- centration of fatty acids in the pools of free fatty acids in the media (diamonds), of free fatty acids in the cells (square) and of esterified fatty acids (triangle). The error ... concomitant decrease of extracellular fatty acids. Therefore, besides the import of exogenous fatty acids, additional release of internal fatty acids ha...
Ngày tải lên: 07/03/2014, 05:20