... Biochem 157 , 375–380.Y. Matsuo et al. Roles of the HsRad51 L1 and L2 loopsFEBS Journal 273 (2006) 3148– 3159 ª 2006 The Authors Journal compilation ª 2006 FEBS 3159 Roles of the human Rad51 L1 and ... 6, 10, 14, 18, 22, 26, and 30), 2 lM (lanes 3, 7, 11, 15, 19, 23, 27, and 31), and 4 lM (lanes 4, 8, 12, 16, 20, 24, 28, and 32).Y. Matsuo et al. Roles of the HsRad51 L1 and L2 loopsFEBS Journal ... HsRad51-L1 loop and measured the fluorescence changes of the tryptophanresidues upon DNA binding.ResultsStrategy of mutational analysisIn order to study the functions of the L1 and L2loops of HsRad51,...