Báo cáo khoa học: Homoadenosylcobalamins as probes for exploring the active sites of coenzyme B12-dependent diol dehydratase and ethanolamine ammonia-lyase docx

... use them as probes for exploring the active sites of enzymes, the coenzymic properties of homoadenosylcobalamins for diol dehydratase and ethanol- amine ammonia-lyase were investigated. The k cat and ... [24] and AdoEtCbl [29] were reported to be totally inactive as coenzyme for ribonucleotide reductase, and the latter inactive for diol deh...

Ngày tải lên: 07/03/2014, 21:20

... Fe(NH 4 ) 2 (SO 4 ) 2 and 0.1 mL of 2.5 m KSCN, using H 2 O 2 as a standard. The amount of the ferrithiocya- nate complex present was determined by measurement of A 490 . The percentage of peroxides removed was ... from the S. solfa- taricus P2 genome: we report on the cloning of bcp1, bcp3 and bcp4 and the characterization of the recombi- nant products and,...

Ngày tải lên: 07/03/2014, 05:20

... poly- morphism of the (A ⁄ T) domain and the dynamics of the bent form one determinant for specific complex formation. They appear to be as important as the con- servation of the DNA base sequence. Therefore, ... tem- peratures. The increase in intensity of the 926 (924), 1444 and 1462 cm )1 vibrational bands of deoxyribose and of the 790 and 1056 cm )...

Ngày tải lên: 07/03/2014, 09:20

... Hafer and Weiss, 1974). Another way of classifying ap- proaches is based on the learning aspect during the construction of the morphological model. If the data for training the model has the same ... precision= f -measure·recall 2recall−f -measure and are hy- perboles. For increasing f-measure values the iso- metrics are moving further to the top-right corner of...

Ngày tải lên: 20/02/2014, 04:20

... are known sites of nanoparticle accumulation, could provide useful measures of nanoparticle safety. Such assays would expand the current platform of tests for the determination of the biocompatibility ... lgÆmL )1 ) in the presence of the fatty acid synthase (FAS) inhibi- tor cerulenin (5 lgÆmL )1 ). FAS is responsible for the synthesis of FFAs, which can then be...

Ngày tải lên: 07/03/2014, 00:20

... conformational stability of the 1C zymogen increases on activation, perhaps as the result of the release of strain. Affinity for ribonuclease inhibitor and cytotoxicity RI recognizes members of the ... provides a measure of the therapeutic index of a ribonuclease zymogen. For the 1C zymogen, the (k cat ⁄ K m ) activated ⁄ (k cat ⁄ K m ) unactivated ratio is 10...

Ngày tải lên: 07/03/2014, 11:20

... replicases in the eukary- otic cell [22]. Therefore, we tested the effect of the replicative state of the cell on the interaction between Pol e and RNA pol II. A possible confinement of the interaction ... between the hypophosphorylated initiation isoform IIA and the hyperphosphorylated elongating isoform IIO that exhibits reduced electrophoretic mobility compared wit...

Ngày tải lên: 07/03/2014, 11:20

... indicate the positions of dHAND mutants. (B) Coimmunoprecipitation assay of dHAND and Akt. AU5-dHAND was immunoprecipitated from the cell lysate of 293T c ells e xpressing AU5-dHAND and HA-Akt. These samples ... of dHAND-WT/E47. The activity of dHAND-Ala-X/E47 was p artially reduced by coexpression of Akt. But this inhibitory effect of Akt was not detected in the cas...

Ngày tải lên: 07/03/2014, 16:20

... significantly increases the cost of the purification, and the require- ment for HPLC increases the length and complexity of the purification procedure. Moreover, as with the studies by Lee et al. [21] and Subramanian ... Subramanian and Shree [42], there was no rigorous assessment of the purity of the product or the correctness of the sequence. In con- t...

Ngày tải lên: 18/02/2014, 13:20

... phrase tables with these two meth- ods, and then respectively use them in place of the out -of- domain phrase table to test the system perfor- mance. For the purpose of studying the generality of our ... probabilities, has a great effect on the performance of SMT system. Phrase probability measures the co-occurrence frequency of a phrase pair, and lexical probabili...

Ngày tải lên: 19/02/2014, 19:20