Báo cáo khoa học: Cholecystokinin rapidly stimulates CrkII function in vivo in rat pancreatic acini Formation of CrkII–protein complexes docx

... Cholecystokinin rapidly stimulates CrkII function in vivo in rat pancreatic acini Formation of CrkII protein complexes Alberto G. Andreolotti1, Maria ... activation of the G-protein coupled CCKAreceptor in vivo in rat pancreatic acini promotes the function of CrkII, resulting in complex formation (CrkII paxillin and CrkII p130Cas). Formation of both ... formation of CrkII protein complexes in rat pancreatic acini. Thus, in the present work,we investigated whether in vivo activation of the CCKAreceptor regulates CrkII function to form protein complexes in...

Báo cáo khoa học: Expression level and agonist-binding affect the turnover, ubiquitination and complex formation of peroxisome proliferator activated receptor b pptx

... occurredmainly in protein complexes of approximately 2 MDa(fraction 16). The same fraction contained only verylow levels of RxRa in comparison to PPAR b, indicat-ing that these complexes are ... starved for45 min in methionine ⁄ cysteine-free DMEM (Invitrogen,Karlsruhe, Germany) containing 1% glutamine and 5%dialyzed fetal bovine serum, and incubated with 430 lCi of Redivue ProMix ... exam-ple, in protecting the cell against the overexpression of PPARb in pathophysiological conditions. Further-more, our ﬁndings indicate that the experimental dataobtained by the overexpression of...

Báo cáo khoa học: Unique proteasome subunit Xrpn10c is a speciﬁc receptor for the antiapoptotic ubiquitin-like protein Scythe docx

... regulation of apoptosis. In this study, weidentiﬁed two distinct domains in the N-terminalregion of Scythe capable of binding Xrpn10c redun-dantly: Domain I and Domain II. Domain I containsa typical ... domains in bothDomain I and Domain II are involved in targeting of Scythe to Xrpn10c in vivo. These results indicate thatScythe is a novel protein that contains functional tan-dem ubiquitin homology ... Xrpn10cbinding, indicating that the BAG domain at the C-ter-minus of Scythe is not necessary for Xrpn10c binding(Fig. 5A,B). In good agreement with these in vivo observations, an in vitro...

Báo cáo khoa học: Vitamin D stimulates apoptosis in gastric cancer cells in synergy with trichostatin A ⁄sodium butyrate-induced and 5-aza-2¢-deoxycytidine-induced PTEN upregulation ppt

... of a novel option foraccessory cancer therapy involving the combined use of vitamin D and epigenetic modiﬁers. This strategy alsohas the advantage of the use of low concentrations of vitamin ... openchromatin structure to facilitate the binding of majortranscription factors on the promoter. We weretherefore interested in determining whether TSA ⁄ -NaBu had any effects on promoting the binding ... HDAC inhibitors canimprove the sensitivity of actinotherapy and chemo-therapy [32,33].The main aim of this study was, on the basis of theabove information, to determine whether vitamin Dhad...

Báo cáo khoa học: "Medical emergency teams: deciphering clues to crises in hospitals"

... following a trend set in Australia. The medical literature is now rapidly growing aswell, but it has been focused almost exclusively on either thebenefits of METs in terms of reducing unexpected mortality ... Care Medicine and Internal Medicine, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USACorresponding author: Michael DeVita, devitam@upmc.eduPublished online: 18 May ... there are norandomized clinical trials showing benefit from introduction of METs, many single center reports [2-4] support the notionthat timely intervention may interrupt crisis events anddecrease...

Tài liệu Báo cáo khoa học: The splicing factor ASF/SF2 is associated with TIA-1-related/ TIA-1-containing ribonucleoproteic complexes and contributes to post-transcriptional repression of gene expression doc

... dis-tribution of mutants defective for RNA binding. Pointmutations disrupting the RNA-binding capacity of any of the two RRMs led to the nuclear sequestration of the reporter protein, conﬁrming the inability ... theabundance of each ARE-BP [53]. Interestingly, thedown-regulating effect of ASF ⁄ SF2 is preserved uponinactivation of its RNA-binding ability by point muta-tions in both RRM RNA-binding motifs ... components of the post-transcriptional regulation of gene expression.These proteins are predominantly composed of well-conserved RNA-binding domains mediating RNA con-tact, and auxiliary domains involved...

Tài liệu Báo cáo khoa học: Collagen I regulates matrix metalloproteinase-2 activation in osteosarcoma cells independent of S100A4 pdf

... Novelfunctions of TIMPs in cell signaling. Cancer MetastasisRev 25, 99–113.45 Stetler-Stevenson WG (2008) Tissue inhibitors of metal-loproteinases in cell signaling: metalloproteinase-inde-pendent ... regarding the role of theaVb3 integrin, because it is also reported to suppresscollagen I-induced activation of proMMP-2 [8].Another MMP inhibitor, reversion-inducing cysteine-rich protein with ... Second, wewanted to investigate whether the cysteine proteinaselegumain is involved in the activation. The presence of this proteinase was shown in both cell lines, but incu-bation on monomeric...

Tài liệu Báo cáo khoa học: An ecdysteroid-inducible insulin-like growth factor-like peptide regulates adult development of the silkmoth Bombyx mori docx

... Polonsky KS (1993) Plasma insulin,C-peptide, and proinsulin concentrations in obeseand nonobese individuals with varying degrees of glucose tolerance. J Clin Endocrinol Metab 76, 44–48.25 Baker ... havedistinct domain organizations and physiological func-tions: insulin is a heterodimeric peptide consisting of an A-chain and a B-chain, whereas IGFs are single-chain peptides with domains B, ... bombyxin-like peptide; BIGFLP, Bombyx mori insulin-like growth factor-like peptide; BrdU,bromodeoxyuridine; DAPI, 4¢,6-diamidino-2-phenylindole; DILP, Drosophila insulin-like peptide; IGF, insulin-like...

Tài liệu Báo cáo khoa học: Amino acid discrimination by arginyl-tRNA synthetases as revealed by an examination of natural speciﬁcity variants doc

... December2008)doi:10.1111/j.1742-4658.2009.06866.xl-Canavanine occurs as a toxic non-protein amino acid in more than 1500leguminous plants. One mechanism of its toxicity is its incorporation intoproteins, replacing l-arginine and giving rise to functionally ... soybean. When binding of arginine in thepresence of tRNA was investigated, some changes in the binding architecture were observed [35], in thatN(153:Y), in addition to interacting with the a-carbox-ylate, ... level attained in the pres-ence of L-arginine was compared to that in the presence of 1 mM of the analogue indicated, using [32P]-labelled jack bean transcript tRNA.Inset: Activity of arginyl-tRNA...

Tài liệu Báo cáo khoa học: A knowledge-based potential function predicts the speciﬁcity and relative binding energy of RNA-binding proteins ppt

... successful examples of binding-sequencediscrimination, the native sequences of the RRM1 of Sex-lethal protein (1B7F_1) and KH1 domain of Poly C-binding protein-2 were both ranked ﬁrst out of 256 sequences, ... forpredicting the speciﬁcity of RNA-binding proteins andto evaluate the contribution of individual amino acidsto the energetic of protein–RNA complexes. Knowledge-based potential functions ... sequence-speciﬁc RNA-binding proteins, and recapitulatesexperimentally determined relative changes in binding energy due to muta-tions of individual amino acids at protein–RNA interfaces. Thus,...

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