Báo cáo khoa học: Molecular cloning, expression analysis and functional conﬁrmation of ecdysone receptor and ultraspiracle from the Colorado potato beetle Leptinotarsa decemlineata pdf

... 4114–4128 ª 2005 FEBS Molecular cloning, expression analysis and functional conﬁrmation of ecdysone receptor and ultraspiracle from the Colorado potato beetle Leptinotarsa decemlineata Takehiko ... ecdysone receptor (EcR) and ultraspiracle (USP) of the coleopteran Colorado potato beetle Leptinotarsa decemlineata (LdEcR and LdUSP) was conducted. Amino-acid sequences of the proteins deduced from ... for the molecular interaction with the moltinghormone receptor. Here, we report (a) the determin-ation of primary amino acid structures of EcR and USP from L. decemlineata (b) the analysis of...

Báo cáo khoa học: Molecular cloning, expression and characterization of protein disulﬁde isomerase from Conus marmoreus pdf

... rate of decrease of the linear form, the original data were ﬁttedby Y(t) ¼ e–kt· 100%, where Y is the percentage of the linear form, and t is the refolding time. For the rate of increase of the ... experiments.For the rate of increase of the native form in the presence of cPDI, the original data were ﬁtted by Y(t) ¼ Ymax(1 ) e–kt) · 100%, where Y is the percentage of the native form, and t is the ... formin the presence of cPDI, the original data were ﬁtted by Y(t) ¼ Ymax(1 ) e–kt) · 100%, where Y is the percentage of the native form, and t is the refolding time. For the rate of increase of...

Báo cáo khoa học: Molecular cloning, expression and characterization of cDNA encoding cis-prenyltransferases from Hevea brasiliensis A key factor participating in natural rubber biosynthesis pdf

... PCR at either the 5¢-end or 3¢-end of the coding regions of the two HRT cDNAs. The resulting fragments weresequenced, digested with NcoIandBamHI, and ligated into the NcoI–BamHI vector of pET32b(+), ... pET32b(+), yielding the expression plasmids pETHRT1 and pETHRT2. Each of the expression plasmids was used for transformation of E. coli BL21(DE3), and 1 mL of an overnight culture of the transformant ... the sizes of each of the cDNAs. The expression patterns of HRT1 and HRT2mRNAs among the examined Hevea tissues seem predomi-nant in latex, whereas little expression was detected in leavesand...

Tài liệu Báo cáo khoa học: Molecular cloning, recombinant expression and IgE-binding epitope of x-5 gliadin, a major allergen in wheat-dependent exercise-induced anaphylaxis ppt

... region of the x-5 gliadin gene,PCR primers were designed at the position of the initi-ation and termination codons of the gene based on the nucleotide sequences extracted from a database of wheatexpressed ... WDEIA.We cloned and determined the nucleotide sequence of two x-5 gliadin genes, x-5a and x-5b, from geno-mic DNA of wheat cultivar Norin 61, a Japanese softwheat variety. Neither of the isolated ... includes all of the detected IgE-binding epitope sequences. Afterampliﬁcation of the DNA encoding this half of the x-5a gliadin protein by PCR, the DNA fragment wasinserted into the expression...

Báo cáo khoa học: Molecular cloning of the Matrix Gla Protein gene from Xenopus laevis Functional analysis of the promoter identiﬁes a calcium sensitive region required for basal activity doc

... two exons (IA and IB) in the X. laevis gene, with the site of the intron insertion localizedwithin the 5¢ UTR region of t he xMGP gene (Fig. 1 and Table 2 ). The other introns (2, 3 and 4) are ... conserved sites within the MGP coding sequence(Fig. 2 ). Analysis of the phase of each of the xMGP intronslocated within the coding region revealed that introns 2 and 3 are of phase I while intron ... three-fold with the highe st Ca2+concentrationused(Fig.8).DISCUSSIONIn this study, we present the molecular organization of the ﬁrst nonmammalian M GP gene and the functional analysis of its promoter....

Báo cáo khoa học: Molecular cloning and functional expression of the human sodium channel b1B subunit, a novel splicing variant of the b1 subunit potx

... in the presence orabsence of the b1Bsubunit, whereas, under the sameconditions, the rate of inactivation was increased in the presence of the b1subunit. The effects of b1Bwere furtherassessed ... where Gmaxis the maximumconductance, z is the valence of the process and V½is the midpoint voltage of activation.Ionic current expression levels were determined from batches of oocytes injected ... from the increase in current density on the cell surface induced by b1subunits. The functional differ-ences of the b1, b1A and b1Bsubunits suggest that the C-terminal half of b1 and its...

Báo cáo khoa học: Molecular cloning and characterization of the crustacean hyperglycemic hormone cDNA from Litopenaeus schmitti Functional analysis by double-stranded RNA interference technique pot

... of the sinusgland and the one which gives the name to the family[3]. The main CHH activity is to elevate glucose concen-tration in the hemolymph by a process of glycogen de-gradation in the ... cysteine residues at the same positions asthat of other CHHs of penaeid shrimps (Fig. 1B).Tissue-speciﬁc gene expression of L. schmittiCHH gene The size and expression of the CHH mRNA in differ-ent ... suggest that the complete silencing of the gene transcript could not be achieved due to dilu-tion of dsRNA in the feeding stage of the female tick[31]. On the other hand, Acosta and coworkersobtained...

Báo cáo khoa học: Molecular cloning and functional expression of a gene encoding an antiarrhythmia peptide derived from the scorpion toxin pptx

... work, wedescribe the cloning of the gene sequence of BmKIM, the functional expression of the recombinant toxin in Escheri-chia coli and its effect on the sodium channels of neurons and ventricular ... obtained was the same as the cDNA. Therefore, there is only one intron in the sequenceencoding the signal peptide. The position and structure of BmKIM intron are quite similar to that of other scorpionsodium ... Publishers, the Nether-lands.6. Couraud, F., Jover, E. & Dubois, J.M. (1982) Two types of scorpion toxin receptor sites, one related to the activation, the other to the inactivation of the action...

Tài liệu Báo cáo khoa học: Molecular cloning and characterization of soybean protein disulﬁde isomerase family proteins with nonclassic active center motifs pdf

... organelle and membrane proteins are synthe-sized and folded with the assistance of molecular chap-erones and other folding factors in the endoplasmicreticulum (ER). In many cases, the process of ... of the 53 kDa spot (5.3) was identical to a pI value calcu-lated from the amino acid sequence of GmPDIL-3a, and the isoelectric point of the 57 kDa spot (5.1) wasconsistent with that from the ... GmPDIL-3a and GmPDIL-3b were detected at a density of 1.21 gÆmL)1in the presence of MgCl2. In the presence of EDTA,which releases ribosomes from the rough ER [24], the peaks of GmPDIL-3a and GmPDIL-3b...

Báo cáo khoa học: Molecular cloning and characterization of two soybean protein disulﬁde isomerases as molecular chaperones for seed storage proteins doc

... expression were found in the promoter regions of both GmPDIL-1 and GmPDIL-2. However, the mRNA expression patterns of GmPDIL-1 and GmPDIL-2 were different,suggesting that the expression of these ... b-conglycinin during the course of the folding process of these proteins.ResultsCloning and expression of GmPDIL-1 and GmPDIL-2To clone the soybean ortholog of ArabidopsisPDI-like 1-1 and PDI-like ... formation in the ER and may cause the accumulation of unfolded proglycinin. As the active sites of the PDI family proteins are reduced in the ER in the presence of dithiothreitol, neither GmP-DIL-1...

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