Báo cáo khoa học: Quantitative analysis of ultrasensitive responses pot

... Quantitative analysis of ultrasensitive responses Stefan Legewie, Nils Blu¨thgen and Hanspeter HerzelInstitute for ... alternative deﬁni-tion of the Hill coefﬁcient proposed by Goldbeter &Koshland [12] for the analysis of responses, whoseshape differs from the Hill equation:Fig. 1. Limitations of the Hill approach: ... sensitivity of biochemical responses by means of the response coefﬁ-cient deﬁned in Eqn (1) rather than by ﬁtting the Hillequation.The response coefﬁcient of the Hill equation devoid of basal...

Tài liệu Báo cáo khoa học: Quantitative analysis of the experimental O–J–I–P chlorophyll ﬂuorescence induction kinetics Apparent activation energy and origin of each kinetic step Steve Boisvert, David Joly and Robert Carpentier doc

... superposition of the exponential rise to analyzequantitatively the contribution of each phase. Theamplitude and rate constant of each of the threephases can be calculated by deconvolution of thetraces ... delayin response time of the instrument, these data were notincluded in analyses of FI traces.Data analysis For quantitative analysis, FI traces were tted with the sum of three rst-order kinetics ... series of succes-sive events that lead to the progressive reduction of the quinone molecules located on the acceptor side of PSII [3].The progressive reduction of the acceptor side of PSII...

Tài liệu Báo cáo khóa học: Quantitative analysis, using MALDI-TOF mass spectrometry, of the N-terminal hydrolysis and cyclization reactions of the activation 2 process of onconase pdf

... Quantitative analysis, using MALDI-TOF mass spectrometry, of theN-terminal hydrolysis and cyclization reactions of the activationprocess of onconase2Marc Ribo´1, ... embryos of the frog, Rana pipiens [4].ONC, discovered as a result of its potent anticancer activity[5], is now in Phase III human clinical trials for thetreatment of several types of cancer ... equal volume of saturated sinapinic acid inthe same solvent of the matrix, and 0.5 lL of the mixturewas immediately applied to a sample probe spot.Cyclization reaction as a function of pH and...

Tài liệu Báo cáo khoa học: Proteomic analysis of dopamine and a-synuclein interplay in a cellular model of Parkinson’s disease pathogenesis docx

... Department of Structural and Functional Biology, and Centre of Neuroscience, University of Insubria, Busto Arsizio, Italy2 Department of Biotechnology, University of Verona, Italy3 Department of Clinical ... built and then compared; spots were quanti-ﬁed on the basis of their relative volume (spot volume nor-malized to the sum of the volumes of all the representativespots) and those that consistently ... 2d platinum software, version 5.0 (GEHealthcare). Spots were detected automatically by the soft-ware and manually reﬁned; gels were then matched and theresulting clusters of spots conﬁrmed...

Tài liệu Báo cáo khoa học: Kinetic analysis of effector modulation of butyrylcholinesterase-catalysed hydrolysis of acetanilides and homologous esters pdf

... hydrolysis of o-NTFNAC was recorded at 430 nm(e = 3954 m)1Æcm)1) for 5 min.Acetyl estersKinetic analysis of the BuChE-catalysed hydrolysis of o-NPA was followed by monitoring the rate of appearance of ... activity of BuChEin plasma and tissues could participate in the metabo-lism of these aryl acylamide drugs and xenobiotics.However, the potential detoxiﬁcation role of the AAAactivity of BuChE ... DiscussionAction of reversible effectors on AAA activity of BuChEThe investigation of the effects of the ligands(tyramine, serotonin and benzalkonium) on the AAAand esterase activities of BuChE was...

Tài liệu Báo cáo khoa học: Thermodynamic analysis of porphyrin binding to Momordica charantia (bitter gourd) lectin pptx

... theinteraction of several water-soluble porphyrins with MCL.The thermodynamic forces governing the interaction of some of the porphyrins have been delineated from an analysis of the temperature ... (in subunits). Inset: plo t of 1/DA as a function of thereciprocal total pro tein concen tration. T he reciproca l of the Y-in ter-cept of this plot gave th e value of DA1, the change in a ... shown. A ﬁ t of the CDspectrum of native MCL, obtained by analysing thespectrum using theCDSSTRprogram, is also given (details of the spectral analysis are given b elow). The spectrum of MCL in...

Tài liệu Báo cáo khoa học: Subproteomics analysis of Ca2+-binding proteins demonstrates decreased calsequestrin expression in dystrophic mouse skeletal muscle pdf

... viscosity of t he extract as a result of D NA, 2 lLofDNase I (200 units) was added per 100 lL of buffer [30].Following ﬁltration through two layers of miracloth a nd theaddition of four volumes of ... ÔStains-AllÕ analysis of Fig. 3 and the 1D i mmunoblotting of Fig. 4.As the full-length Dp427 isoform of dystrophin does notenter the second dimension of conventional 2D gels, theexpression level of ... calcium h ypothesis of muscular dystrophy [20–22]. Deﬁciency in the D p427isoform o f d ystrophin r esults in the r eduction of a speciﬁcsubset of sarcolemmal glycoproteins [23,24]. The lack of the...

Tài liệu Báo cáo khoa học: Functional analysis of the basic helix-loop-helix transcription factor DEC1 in circadian regulation ppt

... E-box.Substitution of Arg65 for Ala abolished the interaction of DEC1 with BMAL1 and the binding of DEC1 to the E-box.However, substitution of His57 for Ala did not affect theinteraction of D EC1 w ... Kato11Department of Dental and Medical Biochemistry, Hiroshima University Graduate School of Biomedical Sciences, Hiroshima, Japan;2Department of Physiology, Hokkaido University Graduate School of Medicine, ... for the rhythmic expression of Dec1, and the feedback loop of DEC1 (as well as that of BMAL1) might be interlocked with the core feedback loopto constitute a network of the circadian clock system[11,12,14]....

Tài liệu Báo cáo khoa học: Quantitative estimation of channeling from early glycolytic intermediates to CO2 in intact Escherichia coli pdf

... existence of ‘metabolons’, transient asso-ciations of pathway enzymes in addition to stablecomplexes (e.g. cytochrome complexes of the electrontransport chain or the covalent linkage of tryptophansynthase ... signiﬁcant role in the regulation of metabolism. Whereasthe usual mechanism proposed for channeling is the (often) transient inter-action of sequential pathway enzymes, many of the supporting data comefrom ... utilized whole-cell systems, most often only aqualitative assessment of channeling has been reported. Here we develop amethod for making a quantitative calculation of the fraction channeled inglycolysis...

Tài liệu Báo cáo khoa học: Mutational analysis of plasminogen activator inhibitor-1 Interactions of a-helix F and its neighbouring structural elements regulates the activity and the rate of latency transition pdf

... Formation of the covalent serpin–protease complex involves a Michaelis docking complex,cleavage of the P1–P1¢ peptide bond, linkage of the activesite Ser of the protease to the carboxyl group of ... shown is a typicalone out of a total of at least three.Ó FEBS 2003 Mutational analysis of PAI-1 (Eur. J. Biochem. 270) 1685PAI-1 (N152A) all showed a biphasic loss of activitysuggesting a heterogeneity ... Mutational analysis of plasminogen activator inhibitor-1Interactions of a-helix F and its neighbouring structural elements regulatesthe activity and the rate of latency transitionTroels...

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