Báo cáo khoa học: Quantitative analysis of ultrasensitive responses pot

... Quantitative analysis of ultrasensitive responses Stefan Legewie, Nils Blu ¨ thgen and Hanspeter Herzel Institute for ... alternative defini- tion of the Hill coefficient proposed by Goldbeter & Koshland [12] for the analysis of responses, whose shape differs from the Hill equation: Fig. 1. Limitations of the Hill approach: ... sensitivity of biochemical responses by...

Ngày tải lên: 07/03/2014, 21:20

... superposition of the exponential rise to analyze quantitatively the contribution of each phase. The amplitude and rate constant of each of the three phases can be calculated by deconvolution of the traces ... delay in response time of the instrument, these data were not included in analyses of FI traces. Data analysis For quantitative analysis, FI traces were tted with the...

Ngày tải lên: 19/02/2014, 05:20

... Quantitative analysis, using MALDI-TOF mass spectrometry, of the N-terminal hydrolysis and cyclization reactions of the activation process of onconase 2 Marc Ribo ´ 1 , ... embryos of the frog, Rana pipiens [4]. ONC, discovered as a result of its potent anticancer activity [5], is now in Phase III human clinical trials for the treatment of several types of cancer ... equal vo...

Ngày tải lên: 19/02/2014, 12:20

... Department of Structural and Functional Biology, and Centre of Neuroscience, University of Insubria, Busto Arsizio, Italy 2 Department of Biotechnology, University of Verona, Italy 3 Department of Clinical ... built and then compared; spots were quanti- fied on the basis of their relative volume (spot volume nor- malized to the sum of the volumes of all the representative spo...

Ngày tải lên: 15/02/2014, 01:20

... hydrolysis of o-NTFNAC was recorded at 430 nm (e = 3954 m )1 Æcm )1 ) for 5 min. Acetyl esters Kinetic analysis of the BuChE-catalysed hydrolysis of o-NPA was followed by monitoring the rate of appearance of ... activity of BuChE in plasma and tissues could participate in the metabo- lism of these aryl acylamide drugs and xenobiotics. However, the potential detoxification role...

Ngày tải lên: 18/02/2014, 17:20

... the interaction of several water-soluble porphyrins with MCL. The thermodynamic forces governing the interaction of some of the porphyrins have been delineated from an analysis of the temperature ... (in subunits). Inset: plo t of 1/DA as a function of the reciprocal total pro tein concen tration. T he reciproca l of the Y-in ter- cept of this plot gave th e value of DA 1...

Ngày tải lên: 19/02/2014, 16:20

... viscosity of t he extract as a result of D NA, 2 lLof DNase I (200 units) was added per 100 lL of buffer [30]. Following filtration through two layers of miracloth a nd the addition of four volumes of ... ÔStains- AllÕ analysis of Fig. 3 and the 1D i mmunoblotting of Fig. 4. As the full-length Dp427 isoform of dystrophin does not enter the second dimension of conventional...

Ngày tải lên: 19/02/2014, 16:20

... E-box. Substitution of Arg65 for Ala abolished the interaction of DEC1 with BMAL1 and the binding of DEC1 to the E-box. However, substitution of His57 for Ala did not affect the interaction of D EC1 w ... Kato 1 1 Department of Dental and Medical Biochemistry, Hiroshima University Graduate School of Biomedical Sciences, Hiroshima, Japan; 2 Department of Physiology, Hokkaido Un...

Ngày tải lên: 19/02/2014, 16:20

... existence of ‘metabolons’, transient asso- ciations of pathway enzymes in addition to stable complexes (e.g. cytochrome complexes of the electron transport chain or the covalent linkage of tryptophan synthase ... significant role in the regulation of metabolism. Whereas the usual mechanism proposed for channeling is the (often) transient inter- action of sequential pathway enzymes,...

Ngày tải lên: 19/02/2014, 18:20

... Formation of the covalent serpin– protease complex involves a Michaelis docking complex, cleavage of the P 1 –P 1 ¢ peptide bond, linkage of the active site Ser of the protease to the carboxyl group of ... shown is a typical one out of a total of at least three. Ó FEBS 2003 Mutational analysis of PAI-1 (Eur. J. Biochem. 270) 1685 PAI-1 (N152A) all showed a biphasic loss of...

Ngày tải lên: 20/02/2014, 11:20