Báo cáo khoa học: Molecular dynamics structures of peptide nucleic acidÆDNA hybrid in the wild-type and mutated alleles of Ki-ras proto-oncogene ppt

... Rathinavelan and N. Yathindra 4070 FEBS Journal 272 (2005) 4055–4070 ª 2005 FEBS Molecular dynamics structures of peptide nucleic acidÆDNA hybrid in the wild-type and mutated alleles of Ki-ras proto-oncogene Stereochemical ... destabilization and hence, diminution of the melting temperature (T m ) in the former. Base stacking in the vicinity of...

Ngày tải lên: 07/03/2014, 21:20

... Ca 2+ . Removing the proposed Ca 2+ -coordinating residues in positions 1 and 3 of the EF-hand motif significantly compromised the metal-binding capability of the engi- neered protein, indicating that the ... grafting approach to address this question. As shown in Fig. 1B, the distance between the two termini of the inserted Ca 2+ -binding sites in the model str...

Ngày tải lên: 07/03/2014, 00:20

... relaxin-3 in the brain, and particularly its functions, including the stress response and food intake, are described. Expression of relaxin-3 in the brain Relaxin-3 neurons in the brain Examination ... contains the RXXXRXXI motif in the B chain (B12–B19 in human) for binding to the relaxin recep- tor [3]. Similar to insulin, a mature two-chain peptide is produc...

Ngày tải lên: 29/03/2014, 21:20

... gain insights into the structural dynamics of the DNA-binding domains of two prototypic strains, human papillomavirus strain 16 and the bovine papillomavirus strain 1. The simulations underline ... to alanine of the residues Lys325 and Lys327, located in the b2–b3 loop, produce a decrease in the DNA bind- ing [22] that is restored upon back mutation into arginine...

Ngày tải lên: 16/03/2014, 10:20

... for the logistic regression model. More information about the origin and smoothing of the probabilities is given in Section 6. 4.2 Measuring the tense difficulty Having considered the complexity of ... 1), α 1 and β 1 = 0. Thus, when looking for the proba- bility of a text belonging to the baseline level, it is easy to compute the numerator, since exp(0) = 1. The...

Ngày tải lên: 08/03/2014, 21:20

... bridges in the analogs with respect to their adenosine moieties. Details of the syntheses will be published elsewhere, and we present, in the Supporting information, only the key steps leading to the ... lupin enzyme and of 2.1 lm (3) and 2.5 lm (4) for the human counter- part. These K i values lie in the range of the K m values for Ap 4 A: 2.5 lm for the n...

Ngày tải lên: 16/03/2014, 04:20

... with the carbonyl oxygen of Met46¢ and the side chain of Lys45¢. Furthermore, P3 is Arg in CA-p2 and Thr in p2-NC. As a result, the carbonyl oxygen of P3 Arg interacted with the amide of Asp29 instead ... clinical inhibitors in binding to the mutant PRs. The high- resolution crystal structures of PR, PR V82A and PR I84V complexes indicated that the bindin...

Ngày tải lên: 30/03/2014, 20:20

... cata- lyzes the dioxygen-dependent oxidative deamination of primary and secondary amines (sarcosine, N-ethylgly- cine, and glycine) and d-amino acids (d-alanine and d- proline), yielding the corresponding ... (PDB: 3if9) (blue) structures shows the dif- ferent conformations of the main chain of the a2–a3 loop, see arrows [49]. For the sake of clarity, only the...

Ngày tải lên: 14/02/2014, 14:20

... strains revealed the presence of a single point mutation in 1337 nucleotides of BmIVD. In the sku mutant, the 1127th guanine from the start codon was substituted by thymine and none of the other sites ... acts in the third step of leucine degradation and the sku mutant accumulates branched-chain amino acids in haemolymph, this mutant may be useful in the...

Ngày tải lên: 18/02/2014, 04:20

... substitutions in flanking nucleotides of APBE (lower case). Bold letters in the wild-type sequence indicate the APBE. The first nucleotide of the motif is numbered base one. The individual adenine to guanine ... identified for each APUM in the PFAM analysis. Gray circles represent the localization of repeats in the protein and the numbers indicate the positi...

Ngày tải lên: 18/02/2014, 06:20