Báo cáo khoa học: Local stability identiﬁcation and the role of key acidic amino acid residues in staphylococcal nuclease unfolding ppt

... Local stability identiﬁcation and the role of key acidic amino acid residues in staphylococcal nuclease unfolding Hueih-Min Chen1, Siu-Chiu Chan1, King-Wong Leung1, Jiun-Ming Wu1, ... D83G and D95G). The spectrum of E129G(bold line) is much lower in intensity than the others. Protein con-centration, 0.4 mgÆmL)1.H M. Chen et al. Local stability and key acidic amino acids in ... of G75 withH121 and K9, and of G129 with K110 and K133. Other charged amino acids such as D73, D83 and E101 reinforce the interactions of E75 and E129.H M. Chen et al. Local stability and key...

Tài liệu Báo cáo khoa học: Local stability identiﬁcation and the role of a key aromatic amino acid residue in staphylococcal nuclease refolding pdf

... Local stability identiﬁcation and the role of a key aromatic amino acid residue in staphylococcal nuclease refoldingZhengding Su3, Jiun-Ming Wu1, Huey-Jen Fang1, Tian-Yow Tsong2,3 and ... chains into the compact form of the native state. In this study, the effects of mutating W140 [10]on SNase protein conformation and stability wereKeywordsaromatic amino acid; refolding; stability; staphylococcal ... signiﬁcant role in maintainingSNase tertiary structure.Our CD and DSC data show that the W140 in SNase is the amino acid responsible for the stability of the whole protein. However, in comparison...

Báo cáo khoa học: "Unsupervised Argument Identiﬁcation for Semantic Role Labeling" potx

... Us-ing this information, it further reduces the possiblearguments only to those contained in the minimalclause, and further prunes them according to theirposition in the parse tree. In the ... eachsentence, the arguments are a subset of the con-stituents in the parse tree.We use two representations of an argument: oneis the POS tag sequence of the terminals contained in the argument, the other ... each of the verbs in our development data the minimalclause containing it. A minimal clause is the low-est ancestor of the verb in the parse tree that hasa syntactic label of a clause according...

Báo cáo khoa học: Interaction between Lim15/Dmc1 and the homologue of the large subunit of CAF-1 – a molecular link between recombination and chromatin assembly during meiosis pot

... with the homologue of the largestsubunit of chromatin assembly factor 1 (CAF-1) in the basidiomyceteCoprinopsis cinerea (Coprinus cinereus). Using C. cinerea LIM15/DMC1(CcLIM15) as the bait in ... accumu-lating evidence regarding the role of CAF-1 in chroma-tin assembly following various DNA synthesis events,its involvement in chromatin assembly following mei-otic recombination is unknown. In ... amino acid residues with a molecular mass of 120 kDa. The highly charged KER (lysine/glutamate/arginine-rich;242–360 amino acids) and ED (glutamate/aspartate-rich; 522–578 amino acids) domains in CcCac1L...

Báo cáo khoa học: Hexadecylphosphocholine inhibits phosphatidylcholine biosynthesis and the proliferation of HepG2 cells pot

... enzymes of the CDP–choline pathwayAccording to the observations made above, and bearing in mind that the incorporation of choline into PC might beinhibited by HePC interfering with the biosynthesis ... accumulation of radiolabel in CP and a reduction in the radioactivity associated to CDP–choline,suggesting that some of the enzymes involved in the biosynthesis of PC must be the main target of HePC.Cytosolic ... mol)1) and diﬀerent concentrations of HePC. The incorporation of [14C]choline into cellularlipids and soluble intermediates of the CDP–choline pathway was determined, as described in the Materials...

Báo cáo khoa học: "Sentiment Polarity Identiﬁcation in Financial News: A Cohesion-based Approach" pdf

... polarity. The indicators of affective content have been drawn fromlexical sources, corpora and the world wide web and combined in a variety of ways, including factor anal-ysis and machine learning ... viewpoint as amarket player or an ordinary punter, their attitude touncertainty and risk which can be a key factor in the world of ﬁnance. In order to address these issues for the domain of news ... domain of news impact analy-sis in ﬁnance, in recent years the focus has expandedfrom informational to affective content of text in aneffort to explain the relationship between text and the...

Báo cáo khoa học: Genome-wide identiﬁcation of glucosinolate synthesis genes in Brassica rapa potx

... glucosinolates,based on the amino acids from which they are synthe-sized [23]. Biosynthesis of aliphatic and aromatic gluc-osinolates generally involves three steps (Fig. 1) and begins with the ... H, Gershenzon J & BinderS (2006) Branched-chain aminotransferase4 is part of the chain elongation pathway in the biosynthesis of methionine-derived glucosinolates in Arabidopsis. PlantCell ... glucosinolate-producingplants show evolutionary conservation of the enzymes in the conversion of amino acid to aldoxime in the biosynthesis of cyanogenic glucosides and glucosino-lates. Plant Mol Biol 38, 725–734.82...

... As shown in Figure 1,we utilize the mass point of RGB in the bottom of the image at each clause.3.3 Audio FeaturesA cooking video contains various types of audioinformation, such as instructor’s ... topic identiﬁcation method integrating linguistic and vi-sual information using HMMs. Among severaltypes of videos, in which instruction videos (how-to videos) about sports, cooking, D.I.Y., and ... dominant and supposed to be use-ful for topic identiﬁcation and others can be noise. In the case of analyzing utterances in video, itis natural to utilize visual information as well aslinguistic...

Báo cáo khoa học: "High-precision Identiﬁcation of Discourse New and Unique Noun Phrases" docx

... having speciﬁc syntactic structure,2. appearing in the ﬁrst sentence of some text in the training corpus,3. exhibiting the same pattern as several expres-sions of type (2),4. appearing in the ... cross-validationon these 19 texts in order to ﬁnd the settings with the best precision for the class. Thesesettings are then used to train Ripper on all the 19ﬁles and test on the reserved one. The whole ... currently of 32 features.They can be divided into three groups:1. Syntactic Features. We encode part of speech of the head word and type of the determiner.Several features contain information on the characters,...

Báo cáo khoa học: "Phrase Linguistic Classiﬁcation and Generalization for Improving Statistical Machine Translation" docx

... tocombine both the information of verb forms seen in training and off -the- shelf knowledge for generation.A translation memory can be built with all the seenpairs of instances with their in ectional ... share the samesource phrase, dividing the times we see the pair(˜fj, ˜ei) in the training by the times we see˜fj.Unseen instances. To produce a target instance˜f given the tuple T and ... conveniently.Instance probability. On the one hand, when aphrase of the test sentence is classiﬁed to a class, and then translated, how do we produce the instance of the target class given the tuple T and...

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