Báo cáo khoa học: Characterization of structural and catalytic differences in rat intestinal alkaline phosphatase isozymes pdf

... 631–634. Divergences of rat alkaline phosphatase isozymes T. Harada et al. 2486 FEBS Journal 272 (2005) 2477–2486 ª 2005 FEBS Characterization of structural and catalytic differences in rat intestinal alkaline phosphatase ... cell-type alkaline phosphatase; hPLAP, human placental alkaline phosphatase; IAP, intestinal alkaline phosphatase; M1, metal-b...

Ngày tải lên: 07/03/2014, 17:20

... dot-blot, western blot) and MS analysis (TOF-TOF MS) allowed us to investigate the N-terminal processing of CGA in rat heart extracts. Our data reveal the pres- ence of several vasostatin I-containing and vasosta- tin ... negative inotropism and anti- adrenergic activity), we investigated the presence of vasostatin-containing peptides in rat heart. Rat heart extracts wer...

Ngày tải lên: 07/03/2014, 12:20

... (LB) medium containing ampicillin, and 0.5 mL of the culture was then inoculated into 50 mL of the same medium. A crude protein extract containing GST–mSPS1 and His–mDLP1 (or hDPS1 and hDLP1) was ... heterologous combinations, namely, mSPS1 and hDLP1 or hDPS1 and mDLP1, generated both Q 9 and Q 10 , indicating both components are involved in determining the ubiquinone side c...

Ngày tải lên: 16/03/2014, 23:20

... volume of 1.5 mL at 30 °C for 20 min. The concentration of BPGA inthepresenceof0.25m M NADH was determined using excess rabbit muscle GAPDH and 10 lL of the previous mixture in a final volume of 1 ... the quantity of enzyme necessary to convert 1 lmol of substrate per min at 30 °C. Protein concentrations were determined with the Bio- Rad protein dye reagent, using bovine serum...

Ngày tải lên: 23/03/2014, 20:22

... regulation of activities of substance P and related tachykinins [31], whereas cathepsin D is indispensable for protection of the onset and development of a certain type of neuronal ceroid lipofucinosis ... degradation in the endolysosome system. To investigate the possible involvement of intracellular aspartic proteinases in the dis- ruption of structural and functio...

Ngày tải lên: 23/03/2014, 21:20

... Coexistence of a ‘reactive oxygen cycle’ with the Q cycle in the respiratory chain. A hypothesys for generating, partitioning, targeting and functioning of superoxide in mitochondria. Proceedings of the ... ana- lyzed separately and investigated using specific inhibitors and substrates. The frequency of R phases was significantly inhibited by oligomycin and aurovertin (mitoc...

Ngày tải lên: 30/03/2014, 16:20

... accumulates in cyto- plasmic foci in 5–20% of cells under stress condi- tions (Fig. 5B), suggesting that the N-terminal domain of Lsm1p is sufficient in combination with the Sm and C-terminal domains of ... C-terminal domain deleted is consistent with this (Figs 4 and 5). Hybrid proteins carrying the N-terminus of one protein and the Sm domain of the other localize accord...

Ngày tải lên: 07/03/2014, 02:20

... various types of oligosaccharide. Three distinct immunobands were identified for wild-type Kv3.1 expressed in Sf9 cells. The upper band was the predominant band, and the two lower bands were of minor intensity ... digestions, 48 lL of crude brain membranes was mixed with the following: 40 lLofH 2 O, 0.2 lL of protease inhibitor, and 10 lLof denaturation buffer. This mixture was i...

Ngày tải lên: 16/03/2014, 13:20

... Integration of Large-Scale Linguistic Resources in a Natural Language Understanding System Lewis M. Norton, Deborah A. Dahl, Li Li, and Katharine P. Beals Unisys Corporation 2476 ... development and/ or run time. We discuss issues of achieving compatibility between these disparate resources. 1 NL Engine Natural language processing in the Unisys natural language understand...

Ngày tải lên: 20/02/2014, 18:20

... C-terminal part of the protein contains an RNA- and membrane-binding domain, the KH domain. To investigate whether the binding of Era to 16S rRNA and membrane requires its GTPase activity and whether ... whether the binding of Era to 16S rRNA requires the GTPase activity of the protein and whether the RNA-binding domain can bind RNA in the absence of the GTPase domain. Str...

Ngày tải lên: 31/03/2014, 07:20