Báo cáo khoa học: Characterization of structural and catalytic differences in rat intestinal alkaline phosphatase isozymes pdf
Báo cáo khoa học: Characterization of structural and catalytic differences in rat intestinal alkaline phosphatase isozymes pdf
... 631–634.Divergences of rat alkaline phosphatase isozymes T. Harada et al.2486 FEBS Journal 272 (2005) 2477–2486 ª 2005 FEBS Characterization of structural and catalytic differences in rat intestinal alkaline phosphatase ... cell-type alkaline phosphatase; hPLAP, human placental alkaline phosphatase; IAP, intestinal alkaline phosphatase; M1, metal-binding site 1; M2, metal-binding site 2; M3, metal-binding site 3; ... metal-binding site 3; PLAP, placental alkaline phosphatase; rIAP, rat intestinal alkaline phosphatase; SAP, shrimp alkaline phosphatase; TNAP, tissue-nonspecific alkaline phosphatase. FEBS Journal 272...
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Báo cáo khoa học: Characterization of natural vasostatin-containing peptides in rat heart docx
... dot-blot,western blot) and MS analysis (TOF-TOF MS)allowed us to investigate the N-terminal processing of CGA in rat heart extracts. Our data reveal the pres-ence of several vasostatin I-containing and vasosta-tin ... negative inotropism and anti-adrenergic activity), we investigated the presence of vasostatin-containingpeptides in rat heart. Rat heart extracts were purified by RP-HPLC, and the resulting fractions ... concentration-dependent neg-ative inotropism and inhibition of the b-adrenergic-dependent positive inotropy elicited by the N-terminaldomain of CGA [i.e. human recombinant vasostatin I and bovine CGA(7–57)],...
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Báo cáo khoa học: Characterization of solanesyl and decaprenyl diphosphate synthases in mice and humans docx
... (LB) medium containingampicillin, and 0.5 mL of the culture was then inoculatedinto 50 mL of the same medium. A crude protein extractcontaining GST–mSPS1 and His–mDLP1 (or hDPS1 and hDLP1) was ... heterologous combinations, namely, mSPS1 and hDLP1 or hDPS1 and mDLP1, generated both Q9 and Q10, indicating both components areinvolved in determining the ubiquinone side chain. Thus, we identified ... of pSTVK28. pSTVhDLP1 was digested withBamHI and HindIII and cloned into the same sites of pQE31 to yield pQhDLP1, which was in turn digested withXhoI and HindIII and cloned into the SalI and...
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Báo cáo khoa học: Characterization of native and recombinant A4 glyceraldehyde 3-phosphate dehydrogenase Kinetic evidence for conformation changes upon association with the small protein CP12 pptx
... volume of 1.5 mL at 30 °C for 20 min. The concentration of BPGAinthepresenceof0.25mMNADH was determined usingexcess rabbit muscle GAPDH and 10 lL of the previousmixture in a final volume of 1 ... thequantity of enzyme necessary to convert 1 lmol of substrateper min at 30 °C.Protein concentrations were determined with the Bio-Rad protein dye reagent, using bovine serum albumin asstandard.pH ... 0.01Cysteine (ionizing side chain) 9.1 – 9.5Histidine (ionizing side chain) 6.2Ó FEBS 2003 Kinetics of native and recombinant GAPDHs (Eur. J. Biochem. 270) 131The pH optimum for native and recombinant...
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Báo cáo khoa học: Disruption of structural and functional integrity of a2-macroglobulin by cathepsin E pptx
... regulation of activities of substance P and related tachykinins [31],whereas cathepsin D is indispensable for protection of theonset and development of a certain type of neuronal ceroidlipofucinosis ... degradation in the endolysosome system. To investigate the possibleinvolvement of intracellular aspartic proteinases in the dis-ruption of structural and functional integrity of a2M in theendolysosome ... proteolyticactivities of cathepsin E and cathepsin D. TheHb-hydrolyzing activity of cathepsin Epurified from human erythrocytes and rat spleen and cathepsin D purified from bovinespleen and rat spleen (0.01...
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Báo cáo khoa học: Characterization of depolarization and repolarization phases of mitochondrial membrane potential fluctuations induced by tetramethylrhodamine methyl ester photoactivation pdf
... Coexistence of a ‘reactiveoxygen cycle’ with the Q cycle in the respiratory chain.A hypothesys for generating, partitioning, targeting and functioning of superoxide in mitochondria.Proceedings of the ... ana-lyzed separately and investigated using specific inhibitors and substrates.The frequency of R phases was significantly inhibited by oligomycin and aurovertin (mitochondrial ATP synthase inhibitors), ... duration of D and R phases was 1.05 and 0.77 s, respectively. The rate of fluorescence changes in D and R phases was 15 and 12 gray valuesÆs)1,respectively (Fig. 5C–D). Interestingly, the rate of...
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Báo cáo khoa học: Analysis of Lsm1p and Lsm8p domains in the cellular localization of Lsm complexes in budding yeast ppt
... accumulates in cyto-plasmic foci in 5–20% of cells under stress condi-tions (Fig. 5B), suggesting that the N-terminaldomain of Lsm1p is sufficient in combination withthe Sm and C-terminal domains of ... C-terminal domain deleted is consistentwith this (Figs 4 and 5). Hybrid proteins carrying theN-terminus of one protein and the Sm domain of theother localize according to the N-terminal contri-bution: ... with that of Lsm1p in Lsm818p, and the Sm domain of Lsm1p wasreplaced with that of Lsm8p in Lsm181p. Localization of these mutant proteins GFP-tagged at the N- orC-terminus was examined in live...
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Báo cáo khoa học: Characterization of N-glycosylation consensus sequences in the Kv3.1 channel pot
... varioustypes of oligosaccharide. Three distinct immunobandswere identified for wild-type Kv3.1 expressed in Sf9cells. The upper band was the predominant band, and the two lower bands were of minor intensity ... digestions, 48 lL of crude brain membranes was mixed with the following:40 lLofH2O, 0.2 lL of protease inhibitor, and 10 lLofdenaturation buffer. This mixture was incubated at 100 °Cfor 10 min. The ... theS1–S2 linker resides in the membrane for all Kvs [9].Comparison between the S1–S2 linker of KvAP and mammalian Kvs may be difficult because of differences in the length of their S1–S2 linkers and...
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Tài liệu Báo cáo khoa học: "Integration of Large-Scale Linguistic Resources in a Natural Language Understanding System" pdf
... Integration of Large-Scale Linguistic Resources in a Natural Language Understanding System Lewis M. Norton, Deborah A. Dahl, Li Li, and Katharine P. Beals Unisys Corporation 2476 ... development and/ or run time. We discuss issues of achieving compatibility between these disparate resources. 1 NL Engine Natural language processing in the Unisys natural language understanding ... Conversion of the WordNet KB was also straightforward. WordNet files in Prolog are part of the standard WordNet distribution. Therefore, the bulk of the task involved routine reformatting into...
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Báo cáo khoa học: Characterization of the 16S rRNA- and membrane-binding domains of Streptococcus pneumoniae Era GTPase Structural and functional implications docx
... C-terminal part of the proteincontains an RNA- and membrane-binding domain, the KHdomain. To investigate whether the binding of Era to 16SrRNA and membrane requires its GTPase activity and whether ... whether the binding of Era to 16S rRNA requires the GTPase activity of theprotein and whether the RNA-binding domain can bindRNA in the absence of the GTPase domain. Structurally, itremained to ... membrane-binding domain of Era alsorequired the GTP-binding and GTPase activities of theprotein for function and whether, biochemically, the RNA- and membrane-binding domains overlap.To investigate...
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Từ khóa: báo cáo khoa học mẫubáo cáo khoa học y họcbáo cáo khoa học sinh họcbáo cáo khoa học nông nghiệpbáo cáo khoa học lâm nghiệpbáo cáo khoa học thủy sảnBáo cáo quy trình mua hàng CT CP Công Nghệ NPVchuyên đề điện xoay chiều theo dạngNghiên cứu sự hình thành lớp bảo vệ và khả năng chống ăn mòn của thép bền thời tiết trong điều kiện khí hậu nhiệt đới việt namNghiên cứu vật liệu biến hóa (metamaterials) hấp thụ sóng điện tử ở vùng tần số THzGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitQuản lý hoạt động học tập của học sinh theo hướng phát triển kỹ năng học tập hợp tác tại các trường phổ thông dân tộc bán trú huyện ba chẽ, tỉnh quảng ninhPhát triển mạng lưới kinh doanh nước sạch tại công ty TNHH một thành viên kinh doanh nước sạch quảng ninhPhát triển du lịch bền vững trên cơ sở bảo vệ môi trường tự nhiên vịnh hạ longNghiên cứu, xây dựng phần mềm smartscan và ứng dụng trong bảo vệ mạng máy tính chuyên dùngĐịnh tội danh từ thực tiễn huyện Cần Giuộc, tỉnh Long An (Luận văn thạc sĩ)Thiết kế và chế tạo mô hình biến tần (inverter) cho máy điều hòa không khíChuong 2 nhận dạng rui roTổ chức và hoạt động của Phòng Tư pháp từ thực tiễn tỉnh Phú Thọ (Luận văn thạc sĩ)Quản lý nợ xấu tại Agribank chi nhánh huyện Phù Yên, tỉnh Sơn La (Luận văn thạc sĩ)chuong 1 tong quan quan tri rui roGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtBÀI HOÀN CHỈNH TỔNG QUAN VỀ MẠNG XÃ HỘIQUẢN LÝ VÀ TÁI CHẾ NHỰA Ở HOA KỲ