... indicated strains were grown to stationary phase and then lipids were extracted and separated by TLC. The amount of SE of the wildtype strain was set at 100%. Data are mean values of three independent ... between Ste20p and the SE synthases Are1p and Are2p is specific. Binding between Cla4p and Are1p or Are2p was not observed in a similar set of experiments (data not show...
Ngày tải lên: 18/02/2014, 13:20
... Sigma-Aldrich and Carlo Erba (Milan, Italy), and were of the highest available grade. Acknowledgements Thanks are due to Prof G.L. Sottocasa and Dr Anto- nella Bandiera (University of Trieste) ... legitimate to apply the Scrutton and Utter equation [40] to the inhibition data. Data analyses Data were analysed by means of sigmaplot 2001 (SPSS Science Software Gmbh, Erkrath, Ger...
Ngày tải lên: 20/02/2014, 01:20
Báo cáo khoa học: Regulation of arginase II by interferon regulatory factor 3 and the involvement of polyamines in the antiviral response potx
... ArgII, 5¢-GAT CTGCTGATTGGCAAGAGACAA-3¢ and 5¢-CTAAATTC TCACACGTGCTTGATT-3¢ [50], 362 bp; human and murine ArgI, 5¢-ATTGGCTTGAGAGACGTGGACCCT-3¢ and 5¢-TTGCAACTGCTGTGTTCACTGTTC-3¢, 369 bp; human ODC, ... -TGTTGCTGCTGCCTCTACGTT-3¢ and 5¢-GCTGGCATCCTGTTCCTCTACTT-3¢, 138 bp [51]; human b-actin, 5¢-ACAATGAGCTGCTGGTGGCT-3¢ and 5¢-GATGGGCACAGTGTGGGTGA-3¢; murine b-actin, 5¢-TGGAATCCTGTGGCAT...
Ngày tải lên: 07/03/2014, 21:20
Báo cáo khoa học: Induction of raft-like domains by a myristoylated NAP-22 peptide and its Tyr mutant potx
... lipid rearrangement FEBS Journal 272 (2005) 1792–1803 ª 2005 FEBS 1803 Induction of raft-like domains by a myristoylated NAP-22 peptide and its Tyr mutant Raquel F. Epand 1 , Brian G. Sayer 2 and ... spinning; NAP-22 peptide, the myristoylated amino terminal 19 amino acids of NAP-22 (myristoyl-GGKLSKKKKGYNVNDEKAK-amide); NAP-22, neuronal axonal membrane p...
Ngày tải lên: 07/03/2014, 17:20
Tài liệu Báo cáo khoa học: ¨ Induction of Kruppel-like factor 4 by high-density lipoproteins promotes the expression of scavenger receptor class B type I pptx
... siRNA; siR- NA, PMA-differentiated THP-1 macrophages were transiently trans- fected with siRNA of KLF4. The relative values of all results were determined and expressed as mean ± standard error of ... Moreover, it was shown that ligand activated peroxisome proliferator activated receptor increases SR-BI expression in human mono- cytes and macrophages [16]. As a transcriptional fac...
Ngày tải lên: 18/02/2014, 04:20
Tài liệu Báo cáo khoa học: Induction of uPA gene expression by the blockage of E-cadherin via Src- and Shc-dependent Erk signaling docx
... that FAK plays a role in Decma-induced Erk activation. No interaction of Shc and FAK was detected upon Decma treatment and overexpression of dominant-negative FAK-related non-kinase (FRNK) failed ... GmbH (Basel, Switzer- land), SP600125 was obtained from Biomol (Wangen, Swit- zerland), and TPA, horseradish peroxidase-conjugated antimouse and antirabbit antibodies, ECL reagent,...
Ngày tải lên: 19/02/2014, 05:20
Báo cáo khoa học: Modulation of glucocorticoid receptor-interacting protein 1 (GRIP1) transactivation and co-activation activities through its C-terminal repression and self-association domains pptx
... self-transactivation (AD1 and AD2) and co-activator (AR, ER and TR) functions in HeLa cells. The outcome of the relationship between GRIP1 transactivation and co-activator functions var- ies according ... modulate its putative transacti- vation activities and nuclear receptor co-activator functions. Abbreviations ACTR, activator for thyroid hormone and retinoid receptors; AD,...
Ngày tải lên: 07/03/2014, 12:20
Báo cáo khoa học: Induction of PPARb and prostacyclin (PGI2) synthesis by Raf signaling: failure of PGI2 to activate PPARb potx
... 5¢—CCTTCTCCAACCTCTCCTAC—3¢; Cox-2 reverse, 5¢—AGGGGGTGCCAGTGATAGAG—3 ¢; PPARb forward, 5¢—AAGAGGAGAAAGAGGAAG TGG—3¢; PPARb reverse, 5¢—ATTGAGGAAGAGGCTG CTGA—3¢; actin forward, 5¢—GATGATGATATCGCCGC GCTCGTCGTC—3¢; ... forward, 5¢—CATAAGT TTACTGTTGTGGTTCTA—3¢; cPLA2 reverse, 5 ¢—AGT GTCTCGTTCGCTTCC—3¢; COX-2 forward, 5¢—CCATG GGTGTGAAGGGAAATAA—3¢; COX-2 reverse, 5¢—TTG AAAAACTGATGGGTGAAG—3¢; mPG...
Ngày tải lên: 07/03/2014, 12:20
Tài liệu Báo cáo khoa học: Plasticity of laccase generated by homeologous recombination in yeast docx
... isolate a variant of a M. thermophila laccase capable of resisting a wide array of co-solvents at con- centrations as high as 50% v ⁄ v [14]. In all available examples of molecular evolution of ... recombination assays. (A) Parental (lac1, lac2, lac3 and lac5) and hybrid (lac131, lac232 and lac535) sequences are represented by rectangles of variable lengths. Recombin...
Ngày tải lên: 18/02/2014, 06:20
Tài liệu Báo cáo khoa học: "Evaluation of Machine Translations by Reading Comprehension Tests and Subjective Judgments" doc
... quality to a passage is a fairly complex task. We hoped that by asking subjects to judge sentences rather than passages, and to judge for clarity of meaning only, rather than quality generally, ... was avoided by random selection of the volume and page at which the search for each passage started. How- ever, in order to make up a satisfactory comprehension test, it wa...
Ngày tải lên: 19/02/2014, 19:20