... the template after incubation in increasing con- centrations of RGG3. The concentrations of RGG3 were 0 l M (lane 1), 0.2 lM (lane 2), 0.5 l M (lane 3) and 1 lM (lane 4). Identification of Ewing’s sarcoma ... 250 0.8 1 0.6 0.4 0.2 0 0.8 1 Fraction of RNA bound Fraction of DNA bound Fig. 4. Binding affinity of RGG3 to Htelo or rHtelo. The DNA or RNA concentration was fixed at 1 n M,...
Ngày tải lên: 15/02/2014, 01:20
... Apicella MA & Smith AL (2002) Acylation of the lipooligosac- charide of Haemophilus influenzae and colonization: an htrB mutation diminishes the colonization of human airway epithelial cells. Infect ... h of incubation, the resulting Zeo r colonies were selected for PCR identification using primers b1X and b1B, and the inactivated lpxX mutant was verified by sequencing. Cloning of...
Ngày tải lên: 18/02/2014, 14:20
Tài liệu Báo cáo khoa học: Identification of differentially expressed genes of the Pacific oyster Crassostrea gigas exposed to prolonged thermal stress docx
... biphasic expression pattern, consisting of a strong short- and a moderate long-term response. Moreover, after 7–10 days of exposure, we detected differential expression of a number of genes that ... the hypoxia response control system. Mol Biol Cell 15, 3042–3052. 22 Tomanek L & Somero GN (1999) Evolutionary and acclimation-induced variation in the heat-shock responses of conge...
Ngày tải lên: 18/02/2014, 16:20
Tài liệu Báo cáo khoa học: Identification of a novel matrix protein contained in a protein aggregate associated with collagen in fish otoliths pdf
... desired conformation and orientation. Such mineral structures are called biominerals and cannot be formed by any non-biological environments. Calcium carbonate is one of the most common biominerals, ... large exon. In addition, all of the distinctive domains of the proteins are translated from single exons (supplementary Fig. 1A). Inner ear-specific expression of omm-64 mRNA Expression...
Ngày tải lên: 18/02/2014, 17:20
Tài liệu Báo cáo khoa học: Identification of b-amyrin and sophoradiol 24-hydroxylase by expressed sequence tag mining and functional expression assay docx
... modifi- cation of these carbon skeletons and transfer of the sugar moiety (Fig. 1). More than 80 different types of skeleton are generated at the cyclization step [7]. Successful cloning of oxidosqualene ... exhibit a wide range of structural diversity produced by a sequence of biosynthetic reactions. Cyclization of oxidosqualene is the ini- tial origin of structural diversity...
Ngày tải lên: 19/02/2014, 07:20
Tài liệu Báo cáo khoa học: Identification of GAS-dependent interferon-sensitive target genes whose transcription is STAT2-dependent but ISGF3-independent doc
... & Colamonici OR (1994) Tyr- osine phosphorylation of the alpha and beta subunits of the type I interferon receptor. Interferon-beta selec- tively induces tyrosine phosphorylation of an alpha subunit-associated ... Department of Cell and Molecular Biology, Toronto General Research Institute, University Health Network, and Department of Immunology, University of Toronto, ON...
Ngày tải lên: 19/02/2014, 07:20
Tài liệu Báo cáo khoa học: Identification of membrane-bound serine proteinase matriptase as processing enzyme of insulin-like growth factor binding protein-related protein-1 (IGFBP-rP1/angiomodulin/mac25) doc
... M32. Purification of soluble matriptase Serum-free conditioned medium of DLD-1 cells was col- lected, concentrated by ammonium sulfate precipitation, and subjected to molecular-sieve chromatography on a Cellullofine ... and trypsin also contribute to expression of malignant phenotypes in tumor cells [19,20]. Recently, consider- able attention has been focused on the physiological and...
Ngày tải lên: 19/02/2014, 07:20
Tài liệu Báo cáo khoa học: Identification of ATP-NADH kinase isozymes and their contribution to supply of NADP(H) in Saccharomyces cerevisiae docx
... demonstrated the critical contribution of Pos5p to mitochondrial function and survival at 37 °C and the critical contribution of Utr1p to growth in low iron medium. The contributions of the other two enzymes ... partially contribute to the mitochondrial function only in the absence of the critical contributor (Pos5p), i.e. partial contribution was observed only in the absence of the...
Ngày tải lên: 20/02/2014, 01:20
Tài liệu Báo cáo khoa học: Identification of critical active-site residues in angiotensin-converting enzyme-2 (ACE2) by site-directed mutagenesis docx
... addition, the effect of chloride activation is further addressed as the basis of the differing sensitivities of ACE2 and ACE to anions is not currently understood. Results and Discussion Binding of ... is essential for the chloride activation of ACE [18]. The structure of tACE revealed the location of two buried chloride ions [19]. The second chloride ion (CL2) was found to be b...
Ngày tải lên: 20/02/2014, 01:20
Tài liệu Báo cáo khoa học: Identification of different isoforms of eEF1A in the nuclear fraction of human T-lymphoblastic cancer cell line specifically binding to aptameric cytotoxic GT oligomers ppt
... the mechanism of action of our cytotoxic GT oligonucleotides. In this article, we report the identification of eukaryotic elongation factor 1 alpha (eEF1A) as a nuclear component of the CRC in ... Abbott, C.M. (1998) The lethal mutation of the mouse wasted [wst] is a deletion that abolishes expression of a tissue-specific isoform of translation elongation factor 1alpha, encoded...
Ngày tải lên: 21/02/2014, 00:20