Báo cáo khoa học: Identiﬁcation of small scale biochemical networks based on general type system perturbations pdf

Tài liệu Báo cáo khoa học: Identiﬁcation of Ewing’s sarcoma protein as a G-quadruplex DNA- and RNA-binding protein ppt

... thetemplate after incubation in increasing con-centrations of RGG3. The concentrations of RGG3 were 0 lM (lane 1), 0.2 lM (lane 2),0.5 lM (lane 3) and 1 lM (lane 4). Identiﬁcation of Ewing’s sarcoma ... 2500.810.60.40.200.81Fraction of RNA boundFraction of DNA boundFig. 4. Binding afﬁnity of RGG3 to Htelo orrHtelo. The DNA or RNA concentration wasﬁxed at 1 nM, whereas the concentration of RNase-treated ... structure.Furthermore, inhibition of DNA polymerase on a template containing ahuman telomere sequence in the presence of RGG occurs in an RGGconcentration-dependent manner by the formation of a stabilized...

Tài liệu Báo cáo khoa học: Identiﬁcation of two late acyltransferase genes responsible for lipid A biosynthesis in Moraxella catarrhalis doc

... ApicellaMA & Smith AL (2002) Acylation of the lipooligosac-charide of Haemophilus inﬂuenzae and colonization: anhtrB mutation diminishes the colonization of humanairway epithelial cells. Infect ... h of incubation, theresulting Zeorcolonies were selected for PCR identiﬁcation using primers b1X and b1B, and the inactivated lpxXmutant was veriﬁed by sequencing.Cloning of lpxL and construction ... software (DNASTARInc., Madison, WI, USA).Cloning of lpxX and construction of the knockoutmutant O35ElpxXA DNA sequence containing lpxX was ampliﬁed from thechromosomal DNA of O35E using primers...

Tài liệu Báo cáo khoa học: Identiﬁcation of differentially expressed genes of the Paciﬁc oyster Crassostrea gigas exposed to prolonged thermal stress docx

... biphasic expression pattern, consisting of a strongshort- and a moderate long-term response. Moreover,after 7–10 days of exposure, we detected differentialexpression of a number of genes that ... thehypoxia response control system. Mol Biol Cell 15,3042–3052.22 Tomanek L & Somero GN (1999) Evolutionary andacclimation-induced variation in the heat-shockresponses of congeneric marine ... Response of the Paciﬁc oyster Crassostrea gigas to hydrocarbon con-tamination under experimental conditions. Gene 329,147–157.20 David E, Tanguy A, Pichavant K & Moraga D (2005)Response of...

Tài liệu Báo cáo khoa học: Identiﬁcation of a novel matrix protein contained in a protein aggregate associated with collagen in ﬁsh otoliths pdf

... desiredconformation and orientation. Such mineral structuresare called biominerals and cannot be formed by anynon-biological environments. Calcium carbonate is one of the most common biominerals, ... large exon. In addition, all of thedistinctive domains of the proteins are translated fromsingle exons (supplementary Fig. 1A).Inner ear-speciﬁc expression of omm-64 mRNAExpression of omm-64 ... cut-off). Production of rabbit antiserum raised against the recombinant proteinand afﬁnity puriﬁcation of the antibody were performed byHokkaido System Science (Hokkaido, Japan).Collection of...

Tài liệu Báo cáo khoa học: Identiﬁcation of b-amyrin and sophoradiol 24-hydroxylase by expressed sequence tag mining and functional expression assay docx

... modiﬁ-cation of these carbon skeletons and transfer of thesugar moiety (Fig. 1). More than 80 different types of skeleton are generated at the cyclization step [7].Successful cloning of oxidosqualene ... exhibit a wide range of structural diversity produced by asequence of biosynthetic reactions. Cyclization of oxidosqualene is the ini-tial origin of structural diversity of skeletons in their biosynthesis, ... andthe presence of additional hydroxylases must beconsidered. Fortunately, the aglycone of the majorsoyasaponins is soyasapogenol B, and glycosides of soyasapogenol A are minor saponins in G. max....

Tài liệu Báo cáo khoa học: Identiﬁcation of GAS-dependent interferon-sensitive target genes whose transcription is STAT2-dependent but ISGF3-independent doc

... & Colamonici OR (1994) Tyr-osine phosphorylation of the alpha and beta subunits of the type I interferon receptor. Interferon-beta selec-tively induces tyrosine phosphorylation of an alphasubunit-associated ... Department of Cell and Molecular Biology, Toronto General Research Institute, University Health Network, and Department of Immunology, University of Toronto, ON, Canada2 Division of Signaling ... analysis of the contribution of theSTAT2 DNA binding domain to IFN responses on a global basis, andshows that STAT2 is required for the IFN-inducible activation of the fullspectrum of GAS target...

Tài liệu Báo cáo khoa học: Identiﬁcation of membrane-bound serine proteinase matriptase as processing enzyme of insulin-like growth factor binding protein-related protein-1 (IGFBP-rP1/angiomodulin/mac25) doc

... M32.Puriﬁcation of soluble matriptaseSerum-free conditioned medium of DLD-1 cells was col-lected, concentrated by ammonium sulfate precipitation,and subjected to molecular-sieve chromatography on aCellulloﬁne ... andtrypsin also contribute to expression of malignantphenotypes in tumor cells [19,20]. Recently, consider-able attention has been focused on the physiologicaland pathological functions of a membrane-boundserine ... membranefraction of OVISE cells and conditioned medium of DLD-1cells were separated by SDS ⁄ PAGE on 7.5% gels andassayed as follows. Each sample containing 20 lg proteinwas applied to two lanes on a...

Tài liệu Báo cáo khoa học: Identiﬁcation of ATP-NADH kinase isozymes and their contribution to supply of NADP(H) in Saccharomyces cerevisiae docx

... demonstrated the critical contribution of Pos5p tomitochondrial function and survival at 37 °C and the critical contribution of Utr1p to growth in low iron medium. The contributions of the othertwo enzymes ... partially contribute to the mitochondrial functiononly in the absence of the critical contributor(Pos5p), i.e. partial contribution was observed onlyin the absence of the critical contributor. ... of YCp-UTR1 was con-ﬁrmed by the Ura– phenotype of MK1219 (require-Table 2. Effect of compounds on ATP-NAD kinase activity of Yef1p. The effect of compounds on the activity of Yef1p was stud-ied...

Tài liệu Báo cáo khoa học: Identiﬁcation of critical active-site residues in angiotensin-converting enzyme-2 (ACE2) by site-directed mutagenesis docx

... addition, the effect of chloride activation is further addressed as the basis of the differing sensitivities of ACE2 and ACE to anionsis not currently understood.Results and DiscussionBinding of ... is essential for the chloride activation of ACE[18]. The structure of tACE revealed the location of two buried chloride ions [19]. The second chloride ion(CL2) was found to be bound to a water ... effect of chloride ions on enzyme activitywas investigated (Fig. 4). The hydrolysis of Mca-Fig. 3. Role of His505 and His345 in catalysis. Schematic of theproposed reaction intermediate of ACE2,...

$Tài liệu Báo cáo khoa học: Identiﬁcation of different isoforms of eEF1A in the nuclear fraction of human T-lymphoblastic cancer cell line speciﬁcally binding to aptameric cytotoxic GT oligomers ppt$

Tài liệu Báo cáo khoa học: Identiﬁcation of different isoforms of eEF1A in the nuclear fraction of human T-lymphoblastic cancer cell line speciﬁcally binding to aptameric cytotoxic GT oligomers ppt

... the mechanism of action of our cytotoxic GT oligonucleotides.In this article, we report the identiﬁcation of eukaryoticelongation factor 1 alpha (eEF1A) as a nuclear component of the CRC in ... Abbott, C.M. (1998) The lethalmutation of the mouse wasted [wst] is a deletion that abolishesexpression of a tissue-speciﬁc isoform of translation elongationfactor 1alpha, encoded by the Eef1a2 ... an increase of thephosphorylation state. On the contrary, the modiﬁcation of eEF1A mobility on bidimensional PAGE by increasing itspI value should be associated with the presence of otherpost-translational...

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