Báo cáo khoa học: Saccharomyces cerevisiae Ybr004c and its human homologue are required for addition of the second mannose during glycosylphosphatidylinositol precursor assembly ppt

... Saccharomyces cerevisiae Ybr004c and its human homologue are required for addition of the second mannose during glycosylphosphatidylinositol precursor assembly Anne-Lise Fabre 1 , ... protein (Ybr004cp) required for addition of the second man- nose to GPI precursors. Yeast cells depleted of Ybr004cp exhibit cell wall and morphological abno...

Ngày tải lên: 07/03/2014, 16:20

... and Shain, and plotted vs. the inverse of the scan rate. The plots yielded straight lines, confirming the applicability of the Nicholson and Shain theory to the present system, and the variation of the ... CoCl 2 ,0.6andNa 2 B 2 O 7 , 0.1. The optical density, the concentrations of nitrate [17] and nitrite [18], the pO 2 and the pH of the medium...

Ngày tải lên: 07/03/2014, 15:20

... show the pathway of reduction of coenzyme Q to the semiquinone at the P-site in the presence of antimycin A or my- xothiazol. The semiquinone formed in the presence of myxothiazol is the result of ... respectively, at the N-site and P-site of the Q-cycle [18–21]. Both inhibi- tors enhance the formation of oxygen radicals from the P-site [20,21]. Antimycin...

Ngày tải lên: 06/03/2014, 22:21

... to gene expression. The status of the switch is determined by the state of the upstream regulatory element of the GAL genes in the nucleus. The regulatory protein may bind to the upstream regulatory ... is well characterized at the biochemical and g enetic level. The switch constitutes elements for sensing of galactose and transduction of the measurement...

Ngày tải lên: 07/03/2014, 16:20

... Pip2p–Oaf1p are marked with arrows. Asterisks denote complexes of unknown identity. The reason for the increase in the intensity of the signal for the upper nonspecific complex (arrow and asterisk ... whether the difference between the modest induction level of PEX25 and the much higher one of PEX11 is due to the critical deviation from the consensus of...

Ngày tải lên: 17/03/2014, 03:20

... results of a1,6-mannosi- dase resistance and the second mannose incorporation specificities (Fig. 5). Consequently, the synthesis of product B was started by the addition of a mannose residue at the ... further understanding of the molecular mech- anism of the substrate recognition and enzymatic reac- tion, although the biological function of the second...

Ngày tải lên: 23/03/2014, 10:20

... between the precursor and mature forms. In the presence of YidC, precursor M13P2 is converted into its mature form (Fig. 4B, upper panel, lane 1). In the absence of YidC, the processing of the precursor ... overexpression of the other [24]. The Oxa1 protein of Saccharomyces cerevisiae is the founding member of the YidC ⁄ Oxa1 ⁄ Alb3 family, and wa...

Ngày tải lên: 30/03/2014, 03:20

... created. For the second input utterance, the likelihood value of the utterance given the speaker-independent model and that given the speaker-adapted model are calculated and compared. If the former ... and hardware. These advances include the widespread adoption of a statistical Figure 1 shows a mechanism of state -of- the- art speech recognizers [2]. Com...

Ngày tải lên: 20/02/2014, 18:20

... tree. The algorithm starts at the root category and recursively traverses the tree. N C L C L R R R H C C The CCG category of complements and of the root of the graph is determined from their ... CCG or TAG, and for the usefulness of de- pendency counts extracted from the training data. On the other hand, formalisms such as CCG and TAG a re particularly s...

Ngày tải lên: 08/03/2014, 02:21

... interactions from the carbonyl of the substrate thioester to the 2¢OH of the ribityl chain of the FAD and to the main chain amide nitrogen of the residue corresponding to Glu376 of MCAD. Therefore, in ... These interactions are important in the precise positioning and alignment of the flavin, substrate, and Glu376 for optimal catalysis. In addition, the...

Ngày tải lên: 23/03/2014, 12:20