Báo cáo khoa học: Identification of versican as an isolectin B4-binding glycoprotein from mammalian spinal cord tissue pptx

... Oxford. IB4-binding versican in spinal cord tissue O. Bogen et al. 1102 FEBS Journal 272 (2005) 1090–1102 ª 2005 FEBS Identification of versican as an isolectin B4-binding glycoprotein from mammalian spinal ... unam- biguously as a V2-like variant of versican. Moreover, we demonstrate that the IB4-reactive glycoconjugate and the versican variant can be co-r...

Ngày tải lên: 07/03/2014, 16:20

... gene and 2 lg of control luciferase plasmid DNA (pGL3, Promega) were used for transfection. CAT and luciferase activities were measured as described [17]. DNase I protection assay Rat liver and ... nucleotide translocase and glycerol 3-phosphate Fig. 6. Site-2 and Site-3 elements act in a concerted manner to cause the repression of ANT2 promoter. Transient transfections analysis of th...

Ngày tải lên: 07/03/2014, 15:20

... 9 and 18 lm and k off > 1000 s )1 , much weaker than that with GABARAP [37]. Glycans bind CRT with dissociation constants as low as 435 nm and k off ¼ 0.1 s )1 [38]. This is still significantly ... primary antibodies were mixed and applied simultaneously. The concentration of the antibodies, laser intensity and filter settings were carefully controlled, and the sequential scanning mode...

Ngày tải lên: 16/03/2014, 05:20

... EMSA was performed with RGG3 (lanes 2 and 4) and 32 P-labeled Htelo (lanes 1 and 2) or rHtelo (lanes 3 and 4). (B) EMSA was performed with RGG3 (lanes 2 and 4) and 32 P-labeled rHtelo (lanes 1 and ... with EWS (lanes 2, 4 and 6) and 32 P-labeled Htelo (lanes 3 and 4), dsHtelo (lanes 5 and 6) or ssDNA S (lanes 1 and 2). The structures of DNAs used as probes are indicated above each lane. T...

Ngày tải lên: 15/02/2014, 01:20

... templates and primers: total RNA from O35E (lanes 1 and 3), O35ElpxX (lanes 4 and 6) and O35ElpxL (lanes 7 and 9), and chro- mosomal DNA from O35E (lane 2), O35ElpxX (lane 5) and O35El- pxL (lane 8). ... acyltransferase genes encoding decanoyl transferase and dodecanoyl transferase from M. catar- rhalis serotype A strain O35E, and constructed the corresponding isogenic mutants. Analysis...

Ngày tải lên: 18/02/2014, 14:20

... hybridization Messenger RNA was extracted from mantle and gills of oysters exposed to 13 or 25 °C after 7–10 and 24 days. Two micrograms of mRNA (1 lg from the gills and 1 lg from the mantle) were used as the template ... 6393 D9-desaturase and glutamine synthetase) showed a peak of overexpression at days 14 and ⁄ or 17. In con- trast to observations on gill tissue, mantle leve...

Ngày tải lên: 18/02/2014, 16:20

... substances may be aggregates of proteins and polysaccharides, and may play important roles in formation of the phases and ⁄ or morphologies of the crystals because they consist of acidic glycoproteins and ... macromolecule-64 (OMM-64), was identified. The protein was found to have a molecular mass of 64 kDa, and to contain two tandem repeats and a Glu-rich region. The structure of...

Ngày tải lên: 18/02/2014, 17:20

... suspen- ded in 25 mL of 40% potassium hydroxide and 25 mL of methanol and refluxed for 2 h. Products were extracted with 50 mL of hexane. The hexane layer was washed with 25 mL of saturated sodium ... first identification of triterpene hydroxylase cDNA from any plant species. Successful identification of a b-amyrin and sophoradiol 24-hydroxy- lase from the inducible family of...

Ngày tải lên: 19/02/2014, 07:20

... play an important role in mediating the transcription of these genes. The c-fos gene was examined in this system as an example of a GAS-driven gene whose IFN-inducibility is independent of both ... [3,4]. Arguably the most notable of these cascades is the Janus kinase (Jak)-signal transducer and activator of transcription (STAT) pathway that regulates the transcription of nu...

Ngày tải lên: 19/02/2014, 07:20

... milk and successively treated with an anti-TAF ⁄ IGFBP-rP1 monoclonal antibody (#88) or an antimatriptase monoclonal antibody (M32) as the first anti- body, the second antibody (biotinylated antimouse ... from human bladder carcinoma cells [4,5]. Its cDNA was cloned from human leptomeningial cells as mac25 [6] and from human fibroblasts as prostacyclin-stimulating factor (PSF) [...

Ngày tải lên: 19/02/2014, 07:20