Báo cáo khoa học: Stimulation of p-nitrophenylphosphatase activity of Na+ ⁄ K+-ATPase by NaCl with oligomycin or ATP docx

... respect- ively. The phosphorylation reaction was started by the addition of 32 P i with or without pNPP. The reaction mixture (0.5 mL) for phosphorylation by ATP was composed of 50 lgNa + ⁄ K + -ATPase, 5 mm MgCl 2 ,50mm ... the inhibitory effect of NaCl on pNPPase activity in the presence of NaCl up to 20 mm. In the presence of NaCl at concentrations of more...

Ngày tải lên: 07/03/2014, 16:20

... l M) and ATP or slowly hydrolysable ATP analogues (2.0 m M). (1) Without OsDmc1 protein in presence of ATP; (2) with OsDmc1 protein with ATP; (3) with OsDmc1 protein without ATP; (4) with OsDmc1 ... AMP-PNP; (3) with 1.25 lM of OsDmc1 in presence of 2.0 mM ATP- c-S; (4) with 1.25 lM of OsDmc1 in presence of 2.0 m M of ATP. C. Rajanikant et al. DNA strand ex...

Ngày tải lên: 19/02/2014, 07:20

... not important enzymes for the phosphorylation of DZNep or DZAri. To synthesize the nucleotides of DZNep or DZAri for direct testing on viral enzymes, the enzyme(s) responsible for phosphorylating ... decrease in the phosphory- lation of either [ 3 H]DZNep or [ 3 H]DZAri. Therefore, part of the mode of action of these analogs might be similar to that of AZT, which is conver...

Ngày tải lên: 08/03/2014, 08:20

... negative constraint, corresponds to the major unliganded form of the receptor; the active conformation capable of promoting GDP/GTP exchange on the G-protein is achieved by a fraction of the unliganded receptors ... cells with 200 lL NaCl/ P i plus 0.1% BSA. For the permeabilized cell assay, cells were fixed with 2% NaCl/ P i /paraformaldehyde (UCB, Brussels, Belgium) and then tr...

Ngày tải lên: 08/03/2014, 08:20

... digested with two enzyme pairs (BamHI ⁄ SalIorXhoI ⁄ SalI), each pair cutting at both sides of the linearization breakpoint. Southern detection was per- formed with a probe prepared by nick translation ... characterization of the origin of DNA replication of porcine circovirus. J Virol 71, 2562–2566. 34 Goryo M, Suwa T, Matsumoto S, Umemura T & Itak- ura C (1987) Ser...

Ngày tải lên: 16/03/2014, 05:20

... fragments as inhibitors of furin will undoubtedly pave the way for the devel- opment of therapeutically useful furin inhibitors and for the mechanistic studies of the regulation of furin activity in ... assayed for their inhibitory activity. Among them, the major fractions P2, P3 and P4 have a strong inhibitory activity against furin. The homogeneity of the three fractions was...

Ngày tải lên: 16/03/2014, 13:20

... absence of Lpe10p. The F ⁄ YGMN motif is essential for the Mg 2+ transport activity of Lpe10p The conserved F ⁄ YGMN motif in TM1 of CorA-like proteins cannot be varied without loss of Mg 2+ uptake [4,18]. ... lpe10D cells restored DW close to WT levels, meaning that the loss of Fig. 1. Growth phenotypes of yeast strains with deleted MRS2 and ⁄ or LPE10. Serial d...

Ngày tải lên: 29/03/2014, 21:20

... Science Laboratory 99 Image Gauge program (Fuji Photo Film). Chemiluminescence of the band linearly correlated with the amount of recombinant S100A9 (25 to 200 ng per lane). For quantitation of S100A9 ... gradient of acetonitrile from 28 to 40% (v/v) in 0.05% (v/v) trifluoroacetic acid, evaporated, dissolved in phosphate-buffered saline with 1 m M calcium chloride and stored at – 2...

Ngày tải lên: 30/03/2014, 13:20

... growth factor receptors has been reported for several systems, one of which is the modulation of insulin signaling by avb3 integrin. Plasminogen activator inhibitor type-1 (PAI-1), competes with avb3 ... Inhibition of insulin/VN induced PKB phosphorylation by PAI-1. NIH3T3 cells were incubated overnight with 100 n M echistatin (Ec) or decorsin (Dc), or for 4 h with 30 n M...

Ngày tải lên: 08/03/2014, 08:20

... investigated whether stimulation of PAP I activity is correlated with the affinity of Hfq for the 3¢ end of RNA. We used an RNA fragment corresponding to the 3¢ end of the rpsO transcript, which ... were monitored by counting out the radioactivity. Labelling of the 5¢ end was performed with [ 32 P ]ATP[ a ˜ P] and T4 poly- nucleotide kinase, labeled RNAs were separated from...

Ngày tải lên: 19/02/2014, 16:20