... digested with twoenzyme pairs (BamHI ⁄ SalIorXhoI ⁄ SalI), each pair cutting at bothsides of the linearization breakpoint. Southern detection was per-formed with a probe prepared by nick translation ... characterization of the origin of DNA replication of porcine circovirus. J Virol 71,2562–2566.34 Goryo M, Suwa T, Matsumoto S, Umemura T & Itak-ura C (1987) Serial propagation and purification of chicken ... of the linearization breakpoint(Fig. 5). BamHI ⁄ SalIorXhoI ⁄ SalI double-digestions of the input linear construct yielded three restrictionfragments of approximately 2100, 740 and 890 bp with the...