Báo cáo khoa học: Degradation of chitosans with chitinase B from Serratia marcescens Production of chito-oligosaccharides and insight into enzyme processivity docx

... 538–549 ª 2005 FEBS 549 Degradation < /b> of < /b> chitosans < /b> with < /b> chitinase < /b> B from Serratia marcescens Production of < /b> chito-oligosaccharides and insight into enzyme processivity Audun Sørbotten 1 , Svein ... favourable substrates become scarce. Degradation < /b> of < /b> chitosans < /b> with < /b> varying F A Three high molecular mass...

Ngày tải lên: 07/03/2014, 16:20

... cut at 3 and 4 of < /b> 15 possible x-Tyr cleavage sites, respectively. x-Phe bonds were found to be hydrolyzed by MMP-7 (2 of < /b> 16) and MMP-12 (4 of < /b> 16) but not by MMP-9. The cleavage behavior of < /b> MMP-7, ... 2010 FEBS (2 of < /b> 157 possible x-Ala bonds) but are cleaved to a small extent by MMP-9 (12 of < /b> 157) and even more by MMP-12 (24 of < /b> 157). The de...

Ngày tải lên: 16/02/2014, 14:20

... at r b ¼ 0.02. At r b ¼ 0.04 or 0.06, the relative intensity of < /b> the R 53 band reached about 90% of < /b> the value observed with < /b> unmodified DNA (Fig. 6B) . Such behavior reflected inhibition of < /b> p53 binding ... untreated p2 1b. The behavior of < /b> the PGM4 site was similar to that of < /b> p2 1b, showing practically no inhibition of < /b> p53–PGM4 binding for r b...

Ngày tải lên: 19/02/2014, 05:20

... mole- cules of < /b> OAB also bind, and that binding of < /b> OAB at the substrate-binding site is noncompetitive with < /b> Fig. 6. Inhibition of < /b> L 2 by OAB. (A) Double reciprocal plots of < /b> inhibi- tion by 0 l M ... (nm) Absorbance A 450 nm 500 600 Fig. 2. Characterization of < /b> flavin cofactor. (A) UV–visible absorption spectrum between 300 and 600 nm of < /b> enz...

Ngày tải lên: 07/03/2014, 05:20

... project- ing from the binding pocket (Fig. 3). A potential inter- action between the DA nitrogen and the backbone oxygen of < /b> Leu294 was outweighed by loss of < /b> rotational entropy of < /b> the DA side chain. Table ... conformations, hydrogen bonds formed between the positively charged nitrogen atoms of < /b> NMTIQ and MPTP and the backbone oxygen of < /b> Ser324. The dis-...

Ngày tải lên: 07/03/2014, 05:20

... were both very efficient at decreasing SREBF1 transcript abundance, with < /b> reductions of < /b> 81%, 66% and 69% for SREBF1 ⁄ siRNA1 and reductions of < /b> 79%, 78% and 71% for SREBF1 ⁄ siRNA2 in 14 3B, A549 and ... could be a consequence of < /b> large standard deviations, lack of < /b> standard deviations, and ⁄ or insufficient number of < /b> replicates. Nevertheless, the...

Ngày tải lên: 07/03/2014, 05:20

... short course in bacterial genetics: A laboratory manual and handbook for Escherichia coli and related bacteria. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N Y. 17. Cristobal, S. , de ... translation of < /b> 100TorA/P2TAG13 and 100TorA/ P2TAG24 as f or (B ), carried out in the p resence o r a bsence of < /b> IMVs. Samples with < /b> IMVs were extracted with < /b> sodi...

Ngày tải lên: 07/03/2014, 16:20

... as sets of < /b> attributes. The context probability of < /b> a tag is the product of < /b> the probabilities of < /b> its attributes. The probability of < /b> an attribute given the previous tags is estimated with < /b> a decision ... list of < /b> hand written disambigua- tion rules are used to reduce the number of < /b> tags to one (Klein and Simmons, 1963; Green and Rubi...

Ngày tải lên: 08/03/2014, 21:20

... comparison of < /b> erythrochelin and foroxymithine. Fig. S18. Schematic overview of < /b> Nrps3. Table S1. Bioinformatic overview of < /b> etc gene cluster. Table S2. (A) Comparison of < /b> A 1 and A 4 . (B) Structures of < /b> ... the problem of < /b> a low rate of < /b> biosynthesis and unknown biological activity, we describe a genome mining approach rely- ing on bioinform...

Ngày tải lên: 16/02/2014, 09:20

... DNA-cleaving and RNA-cleaving activities, we found that a number of < /b> nucleotides within the catalytic core and substrate-binding arm of < /b> PL DNAzymes were not highly conserved and could be substituted by ... that bind to both enzyme and substrate. J Mol Biol 310, 723–734. 4 Wang DY, Lai BHY & Sen D (2002) A general strategy for effector-mediated control of < /b> RNA-...

Ngày tải lên: 16/02/2014, 15:20