Báo cáo khoa học: Competition between neighboring topogenic signals during membrane protein insertion into the ER pptx

... 2004) doi:10.1111/j.1432-1033.2004.04394.x To better define the mechanism of membrane protein insertion into the membrane of the endoplasmic reticulum, we measured the kinetics of translocation across microsomal membranes of the N-terminal ... Competition between neighboring topogenic signals during membrane protein insertion into the ER Magnus Monne...

Ngày tải lên: 07/03/2014, 16:20

... FEBS up into the cells was less than the amount adsorbed to the cell surface. The amounts of the other rhodamine dyes were somewhat higher, but still < 3.5 times the amounts adsorbed to the cell ... and the cells were incubated further. At various time points, cell samples were withdrawn and the amount of dye associ- ated with the cells was determined after their sepa...

Ngày tải lên: 18/02/2014, 13:20

... structures without discernible peripheral staining. These punctate structures were fur- ther found to colocalize with the FM4-64 staining endosomes (Fig. 3B). Most interestingly, the C-ter- minal half ... in free sterols and  75% increase in sterol esters [32] and erg3D mutants cannot synthesize ergosterol [33]. Con- sistent with these reports, we observed a 38% increase in total ergoste...

Ngày tải lên: 07/03/2014, 21:20

... determined the structural and functional properties of the protein protein inter- action between v-KIND and MAP2. We defined the binding core regions for the v-KIND–MAP2 interac- tion and showed that the ... (Fig. S2B). Taken together, these results reveal the specific protein protein interaction between the v-KIND KIND2 domain and the middle CD region of MAP2. To narrow...

Ngày tải lên: 14/02/2014, 19:20

... were incubated with 100 l M PLP next to each other in solution. Therefore, heterodimerization would have been possible, and the aim was to either detect it or rule out its occurrence under the ... form hybrid dimers? Secondly, is there a role of interactions between protein and oxyanion during the apofiholo c onversion of CcStP? Complementation of phosphorylase apo-protomers in sol...

Ngày tải lên: 19/02/2014, 16:20

... involved in the interaction as the binding of 125 I-labelled DS was reduced to isogenic derivatives of the AP1 strain lacking either of these surface proteins (Fig. 2). The significance of the M1 protein ... establish the role of M proteins for the GAG interaction we again first focused on the AP1 system. AP1 expresses two members of the M protein family; protein M1 and...

Ngày tải lên: 20/02/2014, 11:20

... formal properties from the Static Gra~mmar, but with more formal defi- nitions of the properties. In the passage from the Static Grammar to the STCG, the form as well as some other characteristics ... tree. The rule then states that the string of terms ~l, ,ctn corresponds (") to the tree B, while the entry cORRESPONDENCE gives the substring-subtree correspond...

Ngày tải lên: 22/02/2014, 10:20

... derivatives are ideal osmolytes, for they neither perturb the denaturation equilibrium nor affect the functional activity under native conditions. However, they have the ability to protect proteins ... monophosphate were also obtained from Sigma. These and other chemicals, which were of analytical grade, were used without further purification. Dialysis and the determination of the co...

Ngày tải lên: 07/03/2014, 00:20

... speculation that proteins might modulate the pK a of the histidine via the hydrogen bond between the His–Asp pair and so ‘fine tune’ the reactivity of AdoCbl. Mutations of either residue result ... coenzyme protein interactions have previously been provided to support the steric effect to explain the cobalt–carbon cleavage mechanism. The binding energy for AdoCbl comes from...

Ngày tải lên: 07/03/2014, 04:20

... that the N-terminus of CcCac1L preferentially interacts with CcLim15. Co-immunoprecipitation experiments showed that these proteins also interact in the crude extract of meiotic cells. Furthermore, ... as a novel interacting partner of CcLim15. Furthermore, it was shown that CcCac1L interacts with CcPCNA during the meiotic prophase. Several DNA synthesis events take place during th...

Ngày tải lên: 07/03/2014, 05:20