Báo cáo khoa học: Structural and functional analysis of the interaction of the AAA-peroxins Pex1p and Pex6p pptx

... of the atoms. The eigenvalues reflect the magnitude of the fluctuation along the respective EVs. Structural superpositions and other conformational analyses were performed using lsq- man [56] and ... the coordinated loop dimension (EV1) and the His156-out dimension (EV2). The tra- jectory reaches the EV1 value of the open sulfate_1* structure (maximum 1.73) an...

Ngày tải lên: 23/03/2014, 07:20

... connectivities between the two subunits of the putative dimer because of extensive overlap in the homonuclear NMR spectra and to the weakness of the NOE signal for molecules of this size. We have therefore used ... in space, and the energy of the system is minimized with respect to the position and orientation of the other mono- mer, along with selected bon...

Ngày tải lên: 23/03/2014, 12:20

... assigned their corresponding binding sites and elucidated the importance of ATP-binding and -hydrolysis of Pex1p and Pex6p for their interaction. We show that the interaction of Pex1p and Pex6p ... for the Pex1p Pex6p interaction and their functional role in peroxisome biogenesis. Results The contribution of Pex1p and Pex6p to peroxisomal bioge...

Ngày tải lên: 07/03/2014, 16:20

... USP7/ HAUSP, and the length of the polyQ region influences the strength of the interaction [163]. Unfortunately, the central part of the Josephin domain is difficult to model because of low sequence ... the stability of t he dimer. The first c luster includes F70/V336 and I72/Q338 ( both in b5 s trand) of D 3 and F 27/Y289 (b2 strand), L67/M324, V70/I327 and...

Ngày tải lên: 30/03/2014, 15:20

... hypoxanthine and guanine is only 0.26% and 0.09%, respectively, of the activity of HPRT, the role of the PRTFDC1 in purine metabolism remains unclear and it is uncertain whether it has the capacity to ... II. The hood domain is mainly built up by residues from the C-terminus and consists of a two-stranded anti-parallel b-sheet composed of b2 and b9 and an a-h...

Ngày tải lên: 15/02/2014, 01:20

... indicate the starting and ending residues of the secondary structural elements. (C) The domain-swapped dimer of St SurE. The A and B subunits are shown in green and blue, respectively. The darker and ... the majority of the residues. There were no disordered regions in the map. The quality of refinement of the two crystal forms is shown in Table 1. Cis p...

Ngày tải lên: 18/02/2014, 14:20

... to the side chain of Asp70, and one from the backbone N of Gly63, and the phosphate group forming hydrogen bonds from O1 to Arg55, from O2 to the backbone N and the side chain of Thr138, and ... Thr197 and Glu204 (Fig. 3A,C), and a hydrophobic interaction between Thr131 and Phe133 (Fig. 3D). The central channel of the hexamer is made up of two layers o...

Ngày tải lên: 18/02/2014, 16:20

... removal of the CUB domains and thereby activation of the growth factor domains. PDGF-C and -D contain both the CUB and growth factor domains when they are secreted and pro- teolytic cleavage is therefore ... lymphoid cell infiltrates of the lung and fibro- sis in the liver [110]. Conclusions The novel members, PDGF-C and -D, of the PDGF subfamily of the cys...

Ngày tải lên: 19/02/2014, 07:20

... F, which protrudes from the core of the monomer and runs anti-parallel to the same strand of a second monomer, allowing for the formation of the dimer. The surface of interaction between monomers ... glutamate and aspar- tate residues confined to the portion of the molecule involving a-helix A, the nearby loop, the first and last turns of helices E and...

Ngày tải lên: 06/03/2014, 00:21

... interaction between strands b2 and b6 of neighboring monomers. The p26 modifications examined in this study are not located in either of these strands and they are not con- sidered further. As the basic p26 ... result of the residue’s location at the edge of the dimer–dimer interface. The maximum oligomer size obtained with p26 R114A was  500 kDa, closer to the mas...

Ngày tải lên: 07/03/2014, 12:20